Data/hora: 19/06/2024 15:10:38

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 26/12/2018
Data da última atualização: | 24/01/2023
Autoria: | OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A.
Afiliação: | LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
Título: | Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.
Ano de publicação: | 2018
Fonte/Imprenta: | Indian Journal of Science and Technology, v. 11, n. 25, 2018.
Páginas: | 9 p.
DOI: | 10.17485/ijst%2F2018%2Fv11i25%2F128251
Idioma: | Inglês
Conteúdo: | Abstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.
Palavras-Chave: | Embryonic Development; Epigenetic Modification; Nuclear Transfer.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/189328/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Effect.pdf
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (24404-1) - UPC - AP - DD -  -  -  - DIS