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| Registros recuperados : 11 | |
| 4. |  | REVERS, L. F.; HENRIQUES, J. A. P.; PASQUALI, G.; REVERS, L. F. Construção de bibliotecas representando genes estádio-específicos durante o desenvolvimento inicial do fruto da cultivar de uva sem semente Thompson Seedless. Brazilian Journal of Plant Physiology, Campinas, v. 19, 2007. Suplemento. Edição dos resumos do XI Congresso Brasileiro de Fisiologia Vegetal, Gramado, 2007. O resumo também foi publicado no: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 55....| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | CARDONE, J. M.; REVERS, L. F.; MACHADO, R. M.; BONATTO, D.; BRENDEL, M.; HENRIQUES, J. A. P. Psoralen-sensitive mutant pso9-1 of Saccharomyces cerevisiae contains a mutant allele of the DNA damage checkpoint gene MEC3. DNA Repair, Amsterdam, v. 5, n. 2, p. 163-171, 2006.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. 179 510-519| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2010. p. 206. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 11. | | MUNARI, F. M.; REVERS, L. F.; CARDONE, J. M.; IMMICH, B. F.; MOURA, D. J.; GUECHEVA, T. N.; BONATTO, D.; LAURINO, J. P.; SAFFI, J.; BRENDEL, M.; HENRIQUES, J. A. P. Sak1 kinase interacts with Pso2 nuclease in response to DNA damage induced by interstrand crosslink-inducing agents in Saccharomyces cerevisiae. Journal of Photochemistry and Photobiology B: Biology, v. 130, p. 241-253, 2014. DOI 10.1016/j.jphotobiol.2013.11.024| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 11 | |
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Registro Completo
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
14/01/2011 |
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Data da última atualização: |
20/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
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Autoria: |
COSTENARO-DA-SILVA, D.; PASSAIA, G.; HENRIQUES, J. A. P.; MARGIS, R.; PASQUALI, G.; REVERS, L. F. |
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Afiliação: |
DANIELLE COSTENARO-DA-SILVA, UFRGS; GISELE PASSAIA, CNPUV (bolsista); JOÃO A. P. HENRIQUES, UFRGS; ROGÉRIO MARGIS, UFRGS; GIANCARLO PASQUALI, UFRGS; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Plant Science, Limerick, v. 179, n. 5, p. 510-519, nov. 2010. |
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Volume: |
179 |
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Páginas: |
510-519 |
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Idioma: |
Inglês |
|
Conteúdo: |
Sultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA. |
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Palavras-Chave: |
Desenvolvimento da baga; Expressão gênica; Identificação genética; PCR em tempo real; RDA; Uva de mesa; Uva sem semente. |
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Thesagro: |
Biologia molecular; Genética; Uva; Viticultura. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25458/1/PlantScience-Revers-2010.pdf
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Marc: |
LEADER 02505naa a2200337 a 4500
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100 1 $aCOSTENARO-DA-SILVA, D.
245 $aIdentification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine.$h[electronic resource]
260 $c2010
300 $a510-519 179
490 $v179
520 $aSultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA.
650 $aBiologia molecular
650 $aGenética
650 $aUva
650 $aViticultura
653 $aDesenvolvimento da baga
653 $aExpressão gênica
653 $aIdentificação genética
653 $aPCR em tempo real
653 $aRDA
653 $aUva de mesa
653 $aUva sem semente
700 1 $aPASSAIA, G.
700 1 $aHENRIQUES, J. A. P.
700 1 $aMARGIS, R.
700 1 $aPASQUALI, G.
700 1 $aREVERS, L. F.
773 $tPlant Science, Limerick$gv. 179, n. 5, p. 510-519, nov. 2010.
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Embrapa Uva e Vinho (CNPUV) |
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