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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/02/2009 |
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Data da última atualização: |
06/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, E. J.; VIEIRA, M. L. C.; GARCIA, A. A. F.; MUNHOZ, C. F.; MARGARIDO, G. R. A.; CONSOLI, L.; MATTA, F. P.; MORAES, M. C.; ZUCCHI, M. I.; FUNGARO, M. H. P. |
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Afiliação: |
Eder Jorge de Oliveira, CNPMF; Maria Lucia C. Vieira, ESALQ/USP; Antônio Augusto F. Garcia, ESALQ/USP; Carla F. Munhoz, ESALQ/USP; Gabriel R. A. Margarido, ESALQ/USP; Luciano Consoli, ESALQ/USP; Frederico P. Matta, ESALQ/USP; Michel C. Moraes, ESALQ/USP; Maria I. Zucchi, IAC; Maria Helena P. Fungaro, UEL. |
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Título: |
An integrated molecular map yellow passion fruit based on simultaneous maximum-likelihood estimation of linkage and linkage phases. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Journal of the American Society Horticultural Science, Mount Vernon, v. 133, n. 1, p. 35-41, jan. 2008. |
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Descrição Física: |
il. |
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ISSN: |
0003-1062 |
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Idioma: |
Inglês |
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Conteúdo: |
The development of genetic maps for auto-incompatible species, such as the yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg.) is restricted due to the unfeasibility of obtaining traditional mapping populations based on inbred lines. For this reason, yellow passion fruit linkage maps were generally constructed using a strategy known as two-way pseudo-testcross, based on monoparental dominant markers segregating in a 1:1 fashion. Due to the lack of information from these markers in one of the parents, two individual (parental) maps were obtained. However, integration of these maps is essential, and biparental markers can be used for such an operation. The objective of our study was to construct an integrated molecular map for a full-sib population of yellow passion fruit combining different loci configuration generated from amplified fragment length polymorphisms (AFLPs) and microsatellite markers and using a novel approach based on simultaneous maximum-likelihood estimation of linkage and linkage phases, specially designed for outcrossing species. Of the total number of loci, 76%, 21%, 0.7%, and 2.3% did segregate in 1:1, 3:1, 1:2:1, and 1:1:1:1 ratios, respectively. Ten linkage groups (LGs) were established with a logarithm of the odds (LOD) score 5.0 assuming a recombination fraction 0.35. On average, 24 markers were assigned per LG, representing a total map length of 1687 cM, with a marker density of 6.9 cM. No markers were placed as accessories on the map as was done with previously constructed individual maps. MenosThe development of genetic maps for auto-incompatible species, such as the yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg.) is restricted due to the unfeasibility of obtaining traditional mapping populations based on inbred lines. For this reason, yellow passion fruit linkage maps were generally constructed using a strategy known as two-way pseudo-testcross, based on monoparental dominant markers segregating in a 1:1 fashion. Due to the lack of information from these markers in one of the parents, two individual (parental) maps were obtained. However, integration of these maps is essential, and biparental markers can be used for such an operation. The objective of our study was to construct an integrated molecular map for a full-sib population of yellow passion fruit combining different loci configuration generated from amplified fragment length polymorphisms (AFLPs) and microsatellite markers and using a novel approach based on simultaneous maximum-likelihood estimation of linkage and linkage phases, specially designed for outcrossing species. Of the total number of loci, 76%, 21%, 0.7%, and 2.3% did segregate in 1:1, 3:1, 1:2:1, and 1:1:1:1 ratios, respectively. Ten linkage groups (LGs) were established with a logarithm of the odds (LOD) score 5.0 assuming a recombination fraction 0.35. On average, 24 markers were assigned per LG, representing a total map length of 1687 cM, with a marker density of 6.9 cM. No markers were placed as accessories on the map as... Mostrar Tudo |
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Palavras-Chave: |
AFLP; flavicarpa; Linkage map integration; Microsatellite; Multipoint distance; Outcrossing specie; Passiflora edulis f. |
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Thesagro: |
Maracujá; Marcador Molecular. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02652naa a2200361 a 4500
001 1655525
005 2023-07-06
008 2008 bl uuuu u00u1 u #d
022 $a0003-1062
100 1 $aOLIVEIRA, E. J.
245 $aAn integrated molecular map yellow passion fruit based on simultaneous maximum-likelihood estimation of linkage and linkage phases.$h[electronic resource]
260 $c2008
300 $cil.
520 $aThe development of genetic maps for auto-incompatible species, such as the yellow passion fruit (Passiflora edulis Sims f. flavicarpa Deg.) is restricted due to the unfeasibility of obtaining traditional mapping populations based on inbred lines. For this reason, yellow passion fruit linkage maps were generally constructed using a strategy known as two-way pseudo-testcross, based on monoparental dominant markers segregating in a 1:1 fashion. Due to the lack of information from these markers in one of the parents, two individual (parental) maps were obtained. However, integration of these maps is essential, and biparental markers can be used for such an operation. The objective of our study was to construct an integrated molecular map for a full-sib population of yellow passion fruit combining different loci configuration generated from amplified fragment length polymorphisms (AFLPs) and microsatellite markers and using a novel approach based on simultaneous maximum-likelihood estimation of linkage and linkage phases, specially designed for outcrossing species. Of the total number of loci, 76%, 21%, 0.7%, and 2.3% did segregate in 1:1, 3:1, 1:2:1, and 1:1:1:1 ratios, respectively. Ten linkage groups (LGs) were established with a logarithm of the odds (LOD) score 5.0 assuming a recombination fraction 0.35. On average, 24 markers were assigned per LG, representing a total map length of 1687 cM, with a marker density of 6.9 cM. No markers were placed as accessories on the map as was done with previously constructed individual maps.
650 $aMaracujá
650 $aMarcador Molecular
653 $aAFLP
653 $aflavicarpa
653 $aLinkage map integration
653 $aMicrosatellite
653 $aMultipoint distance
653 $aOutcrossing specie
653 $aPassiflora edulis f
700 1 $aVIEIRA, M. L. C.
700 1 $aGARCIA, A. A. F.
700 1 $aMUNHOZ, C. F.
700 1 $aMARGARIDO, G. R. A.
700 1 $aCONSOLI, L.
700 1 $aMATTA, F. P.
700 1 $aMORAES, M. C.
700 1 $aZUCCHI, M. I.
700 1 $aFUNGARO, M. H. P.
773 $tJournal of the American Society Horticultural Science, Mount Vernon$gv. 133, n. 1, p. 35-41, jan. 2008.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
03/03/2015 |
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Data da última atualização: |
10/04/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LADINIG, A.; LUNNEY, J. K.; SOUZA, C. J. H.; ASHLEY, C.; PLASTOW, G.; HARDING, J. C. S. |
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Afiliação: |
ANDREA LADINIG, UNIVERSITY OF SASKATCHEWAN, CANADA; JOAN K. LUNNEY, USDA; CARLOS JOSE HOFF DE SOUZA, CNPASA; CAROLYN ASHLEY, UNIVERSITY OF SASKATCHEWAN, CANADA; GRAHAM PLASTOW, UNIVERSITY OF ALBERTA, CANADA; JOHN C. S. HARDING, UNIVERSITY OF SASKATCHEWAN, CANADA. |
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Título: |
Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Veterinary Research, v. 45, 2014. |
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DOI: |
10.1186/s13567-014-0113-8 |
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Idioma: |
Inglês |
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Conteúdo: |
In spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFNα in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFNα was the best indicator of viral load and severity of reproductive PRRSv infection. MenosIn spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimul... Mostrar Tudo |
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Thesagro: |
Porco; Reprodução animal; Respiração; Suino; Virus. |
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Thesaurus NAL: |
Cytokines; Porcine reproductive and respiratory syndrome virus; Swine. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119182/1/cnpasa.pdf
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Marc: |
LEADER 02748naa a2200289 a 4500
001 2010493
005 2018-04-10
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/s13567-014-0113-8$2DOI
100 1 $aLADINIG, A.
245 $aCytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome.$h[electronic resource]
260 $c2014
520 $aIn spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1β), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFNα) and interferon gamma (IFNγ). Three cytokines (IFNα, CCL2, IFNγ) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFNα were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFNα, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFNα, CCL2, and IFNγ in serum, and IFNγ in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFNα in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFNα was the best indicator of viral load and severity of reproductive PRRSv infection.
650 $aCytokines
650 $aPorcine reproductive and respiratory syndrome virus
650 $aSwine
650 $aPorco
650 $aReprodução animal
650 $aRespiração
650 $aSuino
650 $aVirus
700 1 $aLUNNEY, J. K.
700 1 $aSOUZA, C. J. H.
700 1 $aASHLEY, C.
700 1 $aPLASTOW, G.
700 1 $aHARDING, J. C. S.
773 $tVeterinary Research$gv. 45, 2014.
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Embrapa Pesca e Aquicultura (CNPASA) |
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