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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BELESINI, A. A.; CARVALHO, F. M. S.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. S.; CARLIN, S. D.; CAZETTA, J. O.; PINHEIRO, D. G.; FERRO, M. I. T. |
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Afiliação: |
FCAV/Unesp; FCAV/Unesp; FCAV/Unesp; POLIANA FERNANDA GIACHETTO, CNPTIA; FCAV/Unesp; Centro Avançado da Pesquisa Tecnológica do Agronegócio de Cana, Ribeirão Preto, SP; FCAV/Unesp; FCAV/Unesp; FCAV/Unesp. |
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Título: |
De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-20, 2017. |
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DOI: |
http://dx.doi.org/10.4238/gmr16028845 |
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Idioma: |
Inglês |
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Notas: |
Article gmr16028845. |
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Conteúdo: |
ABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 days. Both cultivars perceived the lack of water, but the tolerant cultivar responded more slowly than the sensitive cultivar. The latter requires rapid activation of different water-deficit stress response mechanisms for its survival. This rapid activation of metabolic pathways in response to water stress does not appear to be the key mechanism of drought tolerance in sugarcane. There is still much to clarify on the molecular and physiological pattern of plants in response to drought. MenosABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 da... Mostrar Tudo |
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Palavras-Chave: |
Expressão genética. |
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Thesagro: |
Cana de açúcar; Deficiência hídrica. |
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Thesaurus Nal: |
Drought; Gene expression; Saccharum; Sugarcane; Water stress. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169187/1/AP-Denovo-Belesini-gmr.pdf
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Marc: |
LEADER 03036naa a2200349 a 4500
001 2082783
005 2017-12-18
008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr16028845$2DOI
100 1 $aBELESINI, A. A.
245 $aDe novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress.$h[electronic resource]
260 $c2017
500 $aArticle gmr16028845.
520 $aABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 days. Both cultivars perceived the lack of water, but the tolerant cultivar responded more slowly than the sensitive cultivar. The latter requires rapid activation of different water-deficit stress response mechanisms for its survival. This rapid activation of metabolic pathways in response to water stress does not appear to be the key mechanism of drought tolerance in sugarcane. There is still much to clarify on the molecular and physiological pattern of plants in response to drought.
650 $aDrought
650 $aGene expression
650 $aSaccharum
650 $aSugarcane
650 $aWater stress
650 $aCana de açúcar
650 $aDeficiência hídrica
653 $aExpressão genética
700 1 $aCARVALHO, F. M. S.
700 1 $aTELLES, B. R.
700 1 $aCASTRO, G. M. de
700 1 $aGIACHETTO, P. F.
700 1 $aVANTINI, J. S.
700 1 $aCARLIN, S. D.
700 1 $aCAZETTA, J. O.
700 1 $aPINHEIRO, D. G.
700 1 $aFERRO, M. I. T.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 16, n. 2, p. 1-20, 2017.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas; Embrapa Semiárido. |
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Data corrente: |
30/09/2014 |
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Data da última atualização: |
30/09/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MAIA, C. M. B. de F.; GUIOTOKU, M.; RAUEN, C. C. M.; SIGNOR, D. |
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Afiliação: |
CLAUDIA MARIA BRANCO DE F MAIA, CNPF; MARCELA GUIOTOKU, CNPF; C. C. M. Rauen, UFPR; DIANA SIGNOR DEON, CPATSA. |
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Título: |
Biochar from babassu residues: chemical characterization and thermo gravimetric analysis. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: MEETING OF THE INTERNATIONAL HUMIC SUBSTANCES SOCIETY, 17., 2014, Ioannina. Natural organic matter: structure-dynamics innovative applications: book of abstracts. Ioannina: University os Ioannina, 2014. p. 303-304. |
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Descrição Física: |
Disponibilizado online. |
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Idioma: |
Inglês |
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Conteúdo: |
Babassu is Brazilian palm tree considered the largest native oil resource worldwide. In this work we analyse the lignin, extractives, and holocellulose in the babassu fruit residues (endocarp) and the thermo gravimetric behaviour of the in natura residual biomass, its charcoal and its lignin. Thermogravimetric analysis showed that the removal of extractives and lignin compounds affect the thermal stability of the materials. The biochar from babassu residue is thermally stable and it does not shows functionalized groups in their structure. Besides its potential as C sequester was also demonstated fixed Carbon and thermal stability. |
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Palavras-Chave: |
Biocarvão; Palmeira; Pirólise lenta; Resíduo de babaçu; Resíduo vegetal; Slow pyrolysis. |
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Thesagro: |
Babaçu. |
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Thesaurus NAL: |
biochar. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/109314/1/2014-ClaudiaM-MIHSS-Biochar.pdf
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Marc: |
LEADER 01544nam a2200253 a 4500
001 1996192
005 2014-09-30
008 2014 bl uuuu u00u1 u #d
100 1 $aMAIA, C. M. B. de F.
245 $aBiochar from babassu residues$bchemical characterization and thermo gravimetric analysis.$h[electronic resource]
260 $aIn: MEETING OF THE INTERNATIONAL HUMIC SUBSTANCES SOCIETY, 17., 2014, Ioannina. Natural organic matter: structure-dynamics innovative applications: book of abstracts. Ioannina: University os Ioannina, 2014. p. 303-304.$c2014
300 $cDisponibilizado online.
520 $aBabassu is Brazilian palm tree considered the largest native oil resource worldwide. In this work we analyse the lignin, extractives, and holocellulose in the babassu fruit residues (endocarp) and the thermo gravimetric behaviour of the in natura residual biomass, its charcoal and its lignin. Thermogravimetric analysis showed that the removal of extractives and lignin compounds affect the thermal stability of the materials. The biochar from babassu residue is thermally stable and it does not shows functionalized groups in their structure. Besides its potential as C sequester was also demonstated fixed Carbon and thermal stability.
650 $abiochar
650 $aBabaçu
653 $aBiocarvão
653 $aPalmeira
653 $aPirólise lenta
653 $aResíduo de babaçu
653 $aResíduo vegetal
653 $aSlow pyrolysis
700 1 $aGUIOTOKU, M.
700 1 $aRAUEN, C. C. M.
700 1 $aSIGNOR, D.
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Embrapa Florestas (CNPF) |
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