| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Solos. |
|
Data corrente: |
13/12/2019 |
|
Data da última atualização: |
17/12/2019 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Autoria: |
TABOSA, J. N.; BARROS, A. H. C.; SILVA, F. G. da; BRITO, A. R. de M. B.; SIMÕES, A. L.; MESQUITA, F. L. T. de; NASCIMENTO, M. M. A. do; SILVA FILHO, J. G. da; FRANÇA, J. G. E. de; SILVA, A. B. da; FERRAZ, I.; CARVALHO, E. X. de; CORDEIRO, A. L.; SIMPLÍCIO, J. B. |
|
Afiliação: |
JOSÉ NILDO TABOSA, IPA-PE; ALEXANDRE HUGO CEZAR BARROS, CNPS; FERNANDO GOMES DA SILVA, SEAGRI/DIPAP-AL; ANA RITA DE MORAES BRANDÃO BRITO, IPA-PE; ALUIZIO LOW SIMÕES, IPA-PE; FERNANDO LUCAS TORRES DE MESQUITA, IPA-PE; MARTA MARIA AMÂNCIO DO NASCIMENTO, IPA-PE; JOSÉ GOMES DA SILVA FILHO, COHIDRO-SE; JOSÉ GERALDO EUGÊNIO DE FRANÇA, IPA-PE; ADEMAR BARROS DA SILVA, CNPS; IVAN FERRAZ, IPA-PE; ERIC XAVIER DE CARVALHO, IPA-PE; ANTÔNIO LUIZ CORDEIRO, IFPE; JOSIMAR BENTO SIMPLÍCIO, UFRPE. |
|
Título: |
Importância do melhoramento genético de diferentes tipos de sorgo para as mesorregiões do Agreste, Sertão e afins do Semiárido Brasileiro. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
In: XIMENES, L. F.; SILVA, M. S. L. da; BRITO, L. T. de L. (Ed). Tecnologias de convivência com o Semiárido brasileiro. Fortaleza: Banco do Nordeste do Brasil, 2019. cap. 4, p. 515-569. |
|
Idioma: |
Português |
|
Conteúdo: |
Objetiva-se nesse capítulo explicitar sobre a potencialidade dos diferentes tipos de sorgo obtidos e desenvolvidos para a região do semiárido brasileiro, a partir do programa de melhoramento de sorgo do IPA. Além disso, realizar uma descrição sobre o uso e a aplicabilidade desses diferentes materiais de sorgo granífero, forrageiro, herbáceo e silageiro, enfatizando sua adaptação frente às principais adversidades climáticas ocorrentes na região, no âmbito das cadeias produtivas da bovino, caprino e ovinocultura. Por fim, avaliar também o potencial de cultivares de sorgo sacarino desenvolvidos para a região no contexto da produção da bioenergia. |
|
Thesagro: |
Melhoramento Genético Vegetal; Sorghum Bicolor; Sorgo. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206991/1/Importancia-do-melhoramento-genetico-de-diferentes-tipos-de-sorgo-para-as-mesorregioes-2019.pdf
|
|
Marc: |
LEADER 01794naa a2200313 a 4500
001 2116836
005 2019-12-17
008 2019 bl uuuu u00u1 u #d
100 1 $aTABOSA, J. N.
245 $aImportância do melhoramento genético de diferentes tipos de sorgo para as mesorregiões do Agreste, Sertão e afins do Semiárido Brasileiro.$h[electronic resource]
260 $c2019
520 $aObjetiva-se nesse capítulo explicitar sobre a potencialidade dos diferentes tipos de sorgo obtidos e desenvolvidos para a região do semiárido brasileiro, a partir do programa de melhoramento de sorgo do IPA. Além disso, realizar uma descrição sobre o uso e a aplicabilidade desses diferentes materiais de sorgo granífero, forrageiro, herbáceo e silageiro, enfatizando sua adaptação frente às principais adversidades climáticas ocorrentes na região, no âmbito das cadeias produtivas da bovino, caprino e ovinocultura. Por fim, avaliar também o potencial de cultivares de sorgo sacarino desenvolvidos para a região no contexto da produção da bioenergia.
650 $aMelhoramento Genético Vegetal
650 $aSorghum Bicolor
650 $aSorgo
700 1 $aBARROS, A. H. C.
700 1 $aSILVA, F. G. da
700 1 $aBRITO, A. R. de M. B.
700 1 $aSIMÕES, A. L.
700 1 $aMESQUITA, F. L. T. de
700 1 $aNASCIMENTO, M. M. A. do
700 1 $aSILVA FILHO, J. G. da
700 1 $aFRANÇA, J. G. E. de
700 1 $aSILVA, A. B. da
700 1 $aFERRAZ, I.
700 1 $aCARVALHO, E. X. de
700 1 $aCORDEIRO, A. L.
700 1 $aSIMPLÍCIO, J. B.
773 $tIn: XIMENES, L. F.; SILVA, M. S. L. da; BRITO, L. T. de L. (Ed). Tecnologias de convivência com o Semiárido brasileiro. Fortaleza: Banco do Nordeste do Brasil, 2019. cap. 4, p. 515-569.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Solos (CNPS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
24/03/2009 |
|
Data da última atualização: |
16/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Nacional - A |
|
Autoria: |
MARCELINO, F. C.; GUIMARÃES, M. F. M.; BARROS, E. G. |
|
Afiliação: |
Francismar Correa Marcelino, Embrapa Soja; Marta Fonseca Martins Guimaraes, Embrapa Gado de Leite; Everaldo Gonçalves de Barros, Bioagro / UFV. |
|
Título: |
Detection and quantification of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
Ciência e Tecnologia de Alimentos, Campinas, v. 28, p. 38-45, 2008. |
|
DOI: |
https://doi.org/10.1590/S0101-20612008000500007 |
|
Idioma: |
Inglês |
|
Notas: |
Supl. |
|
Conteúdo: |
The increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit varied from 1.0 to 500 ng, depending on the GM soybean content in the sample. The precision, performance, and linearity were relatively high indicating that the method used for analysis was satisfactory. MenosThe increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit vari... Mostrar Tudo |
|
Palavras-Chave: |
GMO; PCR quantitative; Sausage; Transgenic residues. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596463/1/Detection-and-quantification-of-Roundup-Ready-soybean.pdf
|
|
Marc: |
LEADER 02405naa a2200217 a 4500
001 1596463
005 2024-02-16
008 2008 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0101-20612008000500007$2DOI
100 1 $aMARCELINO, F. C.
245 $aDetection and quantification of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR.$h[electronic resource]
260 $c2008
500 $aSupl.
520 $aThe increasing presence of products derived from genetically modified (GM) plants in human and animal diets has led to the development of detection methods to distinguish biotechnology-derived foods from conventional ones. The conventional and real-time PCR have been used, respectively, to detect and quantify GM residues in highly processed foods. DNA extraction is a critical step during the analysis process. Some factors such as DNA degradation, matrix effects, and the presence of PCR inhibitors imply that a detection or quantification limit, established for a given method, is restricted to a matrix used during validation and cannot be projected to any other matrix outside the scope of the method. In Brazil, sausage samples were the main class of processed products in which Roundup Ready® (RR) soybean residues were detected. Thus, the validation of methodologies for the detection and quantification of those residues is absolutely necessary. Sausage samples were submitted to two different methods of DNA extraction: modified Wizard and the CTAB method. The yield and quality were compared for both methods. DNA samples were analyzed by conventional and real-time PCR for the detection and quantification of Roundup Ready® soybean in the samples. At least 200 ng of total sausage DNA was necessary for a reliable quantification. Reactions containing DNA amounts below this value led to large variations on the expected GM percentage value. In conventional PCR, the detection limit varied from 1.0 to 500 ng, depending on the GM soybean content in the sample. The precision, performance, and linearity were relatively high indicating that the method used for analysis was satisfactory.
653 $aGMO
653 $aPCR quantitative
653 $aSausage
653 $aTransgenic residues
700 1 $aGUIMARÃES, M. F. M.
700 1 $aBARROS, E. G.
773 $tCiência e Tecnologia de Alimentos, Campinas$gv. 28, p. 38-45, 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
