Data/hora: 19/06/2024 02:28:17

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 13/05/2016
Data da última atualização: | 13/05/2016
Autoria: | COUTINHO, T. de C.; GUIMARÃES, M. de A.; VIDAL, M. S.
Afiliação: | TACIANA DE CARVALHO COUTINHO, UFRN; UFAM; MARCIA SOARES VIDAL, CNPAB.
Título: | Isolation and characterization of gene sequence expressed in cotton fiber
Título original: | Isolamento e caracerização de sequências genéticas expressas em fibras de algodão
Ano de publicação: | 2016
Fonte/Imprenta: | Revista Ciência Agronômica, Fortaleza v. 47, n. 2, p. 283-289, abr./jun. 2016
ISSN: | 1806-6690
DOI: | http://dx.doi.org/10.5935/1806-6690.20160033
Idioma: | Inglês
Conteúdo: | Cotton fiber are tubular cells which develop from the differentiation of ovule epidermis. In addition to being one of the most important natural fiber of the textile group, cotton fiber afford an excellent experimental system for studying the cell wall. The aim of this work was to isolate and characterise the genes expressed in cotton fiber (Gossypium hirsutum L.) to be used in future work in cotton breeding. Fiber of the cotton cultivar CNPA ITA 90 II were used to extract RNA for the subsequent generation of a cDNA library. Seventeen sequences were obtained, of which 14 were already described in the NCBI database (National Centre for Biotechnology Information), such as those encoding the lipid transfer proteins (LTPs) and arabinogalactans (AGP). However, other cDNAs such as the B05 clone, which displays homology with the glycosyltransferases, have still not been described for this crop. Nevertheless, results showed that several clones obtained in this study are associated with cell wall proteins, wall-modifying enzymes and lipid transfer proteins directly involved in fiber development
Palavras-Chave: | Arabinogalactana; Biblioteca de cDNA; Glicosiltransferase; Gossypium hirsutum L; Proteínas de transferência.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (40220-1) - UPC - AP - PP - 2016.00072 -  - 2016.00072 - DIS