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Registro Completo |
Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Amazônia Oriental. |
Data corrente: |
08/03/2016 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DOMIT, L. A.; BALBINO, L. C.; MARTINEZ, G. B.; GALERANI, P. R. |
Afiliação: |
LINEU ALBERTO DOMIT, CPAMT; LUIZ CARLOS BALBINO, CPAC; GLADYS BEATRIZ MARTINEZ, CPATU; PAULO ROBERTO GALERANI, DE/P&D. |
Título: |
Transferência de tecnologia em ilpf. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Opiniões, Ribeirão Preto, v. 12, n. 40, p. 12-13, jun./ago. 2015 |
ISSN: |
2177-6504 |
Idioma: |
Português |
Conteúdo: |
Os Programas de Transferência de Tecnologia (TT) em integração lavoura-pecuária-floresta (iLPF) necessitam considerar a complexidade desses sistemas para que efetivamente tenham sucesso. A iLPF é um conjunto de práticas e tecnologias, nos âmbitos agrícola, pecuário e florestal, que interagem entre si e de forma diferente, dependendo das regiões e biomas, condições que devem ser ponderadas na transferência de tecnologia dos sistemas. |
Palavras-Chave: |
Ilpf; Integração lavoura-pecuária-floresta. |
Thesagro: |
Transferência de tecnologia. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01041naa a2200205 a 4500 001 2040054 005 2017-05-23 008 2015 bl uuuu u00u1 u #d 022 $a2177-6504 100 1 $aDOMIT, L. A. 245 $aTransferência de tecnologia em ilpf.$h[electronic resource] 260 $c2015 520 $aOs Programas de Transferência de Tecnologia (TT) em integração lavoura-pecuária-floresta (iLPF) necessitam considerar a complexidade desses sistemas para que efetivamente tenham sucesso. A iLPF é um conjunto de práticas e tecnologias, nos âmbitos agrícola, pecuário e florestal, que interagem entre si e de forma diferente, dependendo das regiões e biomas, condições que devem ser ponderadas na transferência de tecnologia dos sistemas. 650 $aTransferência de tecnologia 653 $aIlpf 653 $aIntegração lavoura-pecuária-floresta 700 1 $aBALBINO, L. C. 700 1 $aMARTINEZ, G. B. 700 1 $aGALERANI, P. R. 773 $tOpiniões, Ribeirão Preto$gv. 12, n. 40, p. 12-13, jun./ago. 2015
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Embrapa Agrossilvipastoril (CPAMT) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
16/12/2011 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
Idioma: |
Inglês |
Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
Palavras-Chave: |
ERIC-PCR; RAPD. |
Thesagro: |
Corynebacterium Pseudotuberculosis. |
Thesaurus NAL: |
caseous lymphadenitis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02507naa a2200265 a 4500 001 1910089 005 2024-02-05 008 2011 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI 100 1 $aGUIMARAES, A. S. 245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource] 260 $c2011 520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. 650 $acaseous lymphadenitis 650 $aCorynebacterium Pseudotuberculosis 653 $aERIC-PCR 653 $aRAPD 700 1 $aDORNELES, E. M. S. 700 1 $aANDRADE, G. I. 700 1 $aLAGE, A. P. 700 1 $aMIYOSHI, A. 700 1 $aAZEVEDO, V. 700 1 $aGOUVEIA, A. M. G. 700 1 $aHEINEMANN, M. B. 773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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