| |
|
|
| Registros recuperados : 175 | |
| 161. |  | MUNK, M.; ZANETTE, R. de S. S.; CAMARGO, L. S. de A.; SOUZA, N. L. G. D. de; ALMEIDA, C. G. de; GERN, J. C.; GUIMARAES, A. S.; LADEIRA, L. O.; OLIVEIRA, L. F. C. de; BRANDAO, H. de M. Using carbon nanotubes to deliver genes to hard-to-transfect mammalian primary fibroblast cells. Biomedical Physics & Engineering Express, v. 3, n. 4, 045002, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
|    |
| 162. | | GUIMARAES, A. S.; GERN, J. C.; LANGE, C. C.; ANDRADE, P. V. D.; MENDONCA, L. C.; ARAUJO, R. S.; MOSQUEIRA, V. C. F.; BRITO, M. A. V. P. e; BRANDAO, H. de M. In vivo test of a nanostructured formulation for control of bovine mastitis. In: Simpósio Embrapa LabEx EUA de Sanidade Animal, 2., 2012, Brasília. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012 p. 25| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 163. | | WELLER, M. M. D. C. A.; FONSECA, I.; SBARDELLA, A. P.; PINTO, I. S. B.; VICCINI, L. F.; BRANDAO, H. de M.; GERN, J. C.; CARVALHO, W. A.; GUIMARÃES, A. S.; BRITO, M. A. V. P.; MUNARI, D. P.; SILVA, M. V. G. B.; MARTINS, M. F. Isolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae. Journal of Dairy Research, v. 86, n. 3, p. 307-314, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 164. | | CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 165. | | GUIMARAES, A. S.; LANGE, C. C.; O'CONNOR, A. M.; CASTANHEIRA, R. G.; HERRMANN, G. P.; VARGAS, A. C.; LIBARDONI, F.; MOSQUEIRA, V. C. F.; SOUZA, G. N. de; MENDONCA, L. C.; BRITO, M. A. V. P. e; GERN, J. C.; BRANDAO, H. de M. Cloxacillin nanostructures formulation for control of clinical bovine keratoconjunctivitis. In: Simpósio Embrapa LabEx EUA de Sanidade Animal, 2., 2012, Brasília. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012 p. 62| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 166. | | GERN, J. C.; GUIMARAES, A. S.; BASSOLI, A. E. G.; HERRMANN, G. P.; VARGAS, A. C.; LIBARDONI, F.; MARTINI, C. L.; BRITO, M. A. V. P. e; LANGE, C. C.; SOUZA, G. N. de; SANTOS, F. F.; SILVA, S. R. da; SILVA, M. A. S.; MOSQUIERA, V. C. F.; BRANDAO, H. de M. Avaliação in vitro de cloxacilina nanoencapsulada contra diferentes isolados de moraxella. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 485-487| Biblioteca(s): Embrapa Gado de Leite. |
|  |
| 167. | | GERN, J. C.; GUIMARAES, A. S.; BASSOLI, A. E.; HERMANN, G. P.; VARGAS, A. C. DE; LIBARDONI, F.; MARTINI, C.; BRITO, M.; LANGE, C. C.; SOUZA, G. N. de; SANTOS, F. F. DOS; SILVA, S. DA; SILVA, M. A. S.; MOSQUEIRA, V. C. F.; BRANDAO, H. de M. Avaliação in vitro de cloxacilina nanoencapsulada contra diferentes isolados de moraxella. Engormix, 20 dez. 2013.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 168. | | ANTUNES, C. A.; GUIMARAES, A. S.; SILVA, W. M.; CASTRO, T. L. DE P.; RIBEIRO, D.; DORELLA, F. A.; SILVA, R. DE F.; GALA-GARCIA, A.; CARVALHO, R. D. DE O.; MORAES, P.; ROCHA, F. S.; MAGALHÃES, A.; ALMEIDA, S. S. DE; EL AOUAR FILHO, R. A.; GOUVEIA, A. M. G.; MIYOSHI, A.; AZEVEDO, V.; SEYFFERT, N. Avaliação de um teste intradérmico de hipersensibilidade com antígenos secretados de Corynebacterium pseudotuberculosis para o diagnóstico subclínico da linfadenite caseosa em caprinos. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 169. | | DORNELES, E. M. S.; SANTANA, J. A.; RIBEIRO, D.; DORELLA, F. A.; GUIMARAES, A. S.; MOAWAD, M. S.; SELIM, S. A.; GARALDI, A. L. M.; MIYOSHI, A.; RIBEIRO, M. G.; GOUVEIA, A. M. G.; AZEVEDO, V.; HEINEMANN, M. B.; LAGE, A. P. Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. Plos One, v. 9, n. 6, e98758, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 170. | | GUIMARAES, A. S.; LANGE, C. C.; O'CONNOR, A. M.; RAPOSO, N. R. B.; SILVA, S. R.; BRANDI, R. R.; HERRMANN, G. P.; VARGAS, A. C.; LIBARDONI, F.; BRITO, M. A. V. P. e; SOUZA, G. N. de; BRANDAO, H. de M. Evaluation of a nanopropolis for control of clinical bovine keratoconjunctivitis. In: Simpósio Embrapa LabEx EUA de Sanidade Animal, 2., 2012, Brasília. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012 p. 60| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 171. | | LIMA, M. O. S.; BRITO, M. A. V. P. e; ALMEIDA, P. A. A.; MESQUITA, B. de S.; LANGE, C. C.; BRANDAO, H. de M.; GUIMARAES, A. S.; GERN, J. C.; SILVA, M. A. S.; MENDONCA, L. C.; QUEIROZ, L. da S.; RIBEIRO, J. B. Ocorrência do gene fnbA que codifica a proteína ligante de fibronectina em Staphylococcus aureus isolados de mastite bovina. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 15., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. (Embrapa Gado de Leite. Documentos, 176.). 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 172. | | DORNELES, E. M. S.; GUIMARÃES, A. S.; GOUVEIA, A. M. G.; COURA, F. M.; CARMO, F. B. do; PAULETTI, R. B.; AZEVEDO, V.; LILENBAUM, W.; VITOR, V. de A.; PINHEIRO, R. R.; FERREIRA, A. C. R.; DASSO, M. G.; LAGE, A. P.; HEINEMANN, M. B. Seroprevalence of Brucella ovis-epididymitis, smooth-Brucella, leptospirosis, toxoplasmosis, and Maedi-Visna in sheep slaughtered in Minas Gerais State, Brazil. Brazilian Journal of Veterinary Research and Animal Science, v. 57, n. 2, e164278, 2020.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 173. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 174. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N. de; LAGE, A. P.; BLAGITZ, M. G.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, v. 36, n. 9, p. 811-818, 2016.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 175. | | BRANDAO, H. de M.; GERN, J. C.; PEREIRA, M. M.; SILVA NETO, A. F. da; LANGE, C. C.; BRITO, M. A. V. P. e; RIBEIRO, J. B.; CARVALHO, W. A.; SOUZA, G. N. de; MENDONCA, L. C.; SILVA, M. V. G. B.; ANDRADE, P. V. D. de; SILVA, M. R.; GUIMARAES, A. S. Terapêutica de Precisão e suas Perspectivas em um País de Pecuária Heterogênea. In: MARTINS, P. do C.; PICCININI, G. A.; KRUG, E. E. B.; MARTINS, C. E.; LOPES, F. C. F. Sustentabilidade ambiental, social e econômica da cadeia produtiva do leite: desafios e perspectivas. Brasília, DF : Embrapa, 2015. p. 311-328| Biblioteca(s): Embrapa Gado de Leite. |
| |
| Registros recuperados : 175 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
29/12/2019 |
|
Data da última atualização: |
06/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
WELLER, M. M. D. C. A.; FONSECA, I.; SBARDELLA, A. P.; PINTO, I. S. B.; VICCINI, L. F.; BRANDAO, H. de M.; GERN, J. C.; CARVALHO, W. A.; GUIMARÃES, A. S.; BRITO, M. A. V. P.; MUNARI, D. P.; SILVA, M. V. G. B.; MARTINS, M. F. |
|
Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
|
Título: |
Isolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Journal of Dairy Research, v. 86, n. 3, p. 307-314, 2019. |
|
DOI: |
https://doi.org/10.1017/s0022029919000451 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection. MenosThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ a... Mostrar Tudo |
|
Palavras-Chave: |
Mammary gland perfused; RNA-Seq. |
|
Thesaurus NAL: |
Dairy cattle; Immune response; Mastitis. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02978naa a2200337 a 4500
001 2117822
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/s0022029919000451$2DOI
100 1 $aWELLER, M. M. D. C. A.
245 $aIsolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae.$h[electronic resource]
260 $c2019
520 $aThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection.
650 $aDairy cattle
650 $aImmune response
650 $aMastitis
653 $aMammary gland perfused
653 $aRNA-Seq
700 1 $aFONSECA, I.
700 1 $aSBARDELLA, A. P.
700 1 $aPINTO, I. S. B.
700 1 $aVICCINI, L. F.
700 1 $aBRANDAO, H. de M.
700 1 $aGERN, J. C.
700 1 $aCARVALHO, W. A.
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aMUNARI, D. P.
700 1 $aSILVA, M. V. G. B.
700 1 $aMARTINS, M. F.
773 $tJournal of Dairy Research$gv. 86, n. 3, p. 307-314, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
