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| 2. |  | MENDES, B. V.; DANTAS, C. M. G.; MACEDO, C. R. de; MATTHEWS, H. R.; BARBOSA, L.; CUNHA, J. N. da; BRANDÃO, J. N. Cartilha do criador de cutia. Mossoró: Escola Superior de Agricultura de Mossoró, 1988. 15 p. (ESAM. Coleção Mossoroense, Série B, 570).| Biblioteca(s): Embrapa Meio-Norte. |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/11/2010 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
GOMES, K. A.; ALMEIDA, T. C.; GESTEIRA, A. da S.; LÔBO, I. P.; GUIMARÃES, A. C. R.; MIRANDA, A. B. de; VAN SLUYS, M. A.; CRUZ, R. S. da; CASCARDO, J. C. M.; CARELS, N. |
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Afiliação: |
Kleber A. Gomes, UESC; Tiago C. Almeida, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Ivon Pinheiro Lobo, UESC; Ana Carolina R. Guimarães, FIOCRUZ; Antonio B. de Miranda, FIOCRUZ; Marie-Anne Van Sluys, USP; Rosenira Serpa da Cruz, UESC; Júlio Cézar Mattos Cascardo, UESC; Nicolas Carels, UESC. |
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Título: |
ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Genomics Insights, v. 3, p. 29?56, 2010. |
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ISSN: |
1178-6310 |
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DOI: |
10.4137/GEI.S4340 |
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Idioma: |
Inglês |
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Notas: |
Diponível em:.Acesso em: 03 nov. 2010. |
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Conteúdo: |
We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas. |
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Palavras-Chave: |
Biofuel; Melhoramento genetico vegatal; Pinhão manso; Terpenes. |
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Thesagro: |
Biocombustível; Jatropha Curcas. |
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Thesaurus NAL: |
alkaloids; fatty acids; genomics. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23536/1/f-2328-GEI-ESTs-from-Seeds-to-Assist-the-Selective-Breeding-of-Jatropha-curcas-L.pdf-31711.pdf
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Marc: |
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100 1 $aGOMES, K. A.
245 $aESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds.$h[electronic resource]
260 $c2010
500 $aDiponível em:<http://www.la-press.com/ests-from-seeds-to-assist-the-selective-breeding-of-jatropha-curcas-l--article-a2328>.Acesso em: 03 nov. 2010.
520 $aWe report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas.
650 $aalkaloids
650 $afatty acids
650 $agenomics
650 $aBiocombustível
650 $aJatropha Curcas
653 $aBiofuel
653 $aMelhoramento genetico vegatal
653 $aPinhão manso
653 $aTerpenes
700 1 $aALMEIDA, T. C.
700 1 $aGESTEIRA, A. da S.
700 1 $aLÔBO, I. P.
700 1 $aGUIMARÃES, A. C. R.
700 1 $aMIRANDA, A. B. de
700 1 $aVAN SLUYS, M. A.
700 1 $aCRUZ, R. S. da
700 1 $aCASCARDO, J. C. M.
700 1 $aCARELS, N.
773 $tGenomics Insights$gv. 3, p. 29?56, 2010.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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