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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/11/2002 |
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Data da última atualização: |
06/11/2002 |
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Autoria: |
ZHANG, J.; MASSMANN, G. A.; MIRABILE, C. P.; FIGUEROA, J. P. |
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Título: |
Nonpregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Biology of Reproduction, v. 60, n. 5, p. 1198-1203, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to characterize the effect of estrogen on lhe expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through lhe jugular with 100 ug/day of estradiol-17Betha for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assar, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's testo The three distinct uterine compartments studied contained lhe mRNA and protein for lhe neuronal (type I NOS) and lhe endothelial (type 111 NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type 111 NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type 111 NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the uterus serves more than one physiological role. In myometrium it mar be a uterorelaxant and regulate glucose utilization, and in endometrium and myometrium it mar regulate blood flow. MenosThe aim of this study was to characterize the effect of estrogen on lhe expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through lhe jugular with 100 ug/day of estradiol-17Betha for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assar, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's testo The three distinct uterine compartments studied contained lhe mRNA and protein for lhe neuronal (type I NOS) and lhe endothelial (type 111 NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type 111 NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type 111 NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the ute... Mostrar Tudo |
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Thesagro: |
Endocrinologia; Estrógeno; Ovino; Prenhez; Reprodução Animal; Útero. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02338naa a2200229 a 4500
001 1528831
005 2002-11-06
008 1999 bl uuuu u00u1 u #d
100 1 $aZHANG, J.
245 $aNonpregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen.
260 $c1999
520 $aThe aim of this study was to characterize the effect of estrogen on lhe expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through lhe jugular with 100 ug/day of estradiol-17Betha for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assar, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's testo The three distinct uterine compartments studied contained lhe mRNA and protein for lhe neuronal (type I NOS) and lhe endothelial (type 111 NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type 111 NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type 111 NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the uterus serves more than one physiological role. In myometrium it mar be a uterorelaxant and regulate glucose utilization, and in endometrium and myometrium it mar regulate blood flow.
650 $aEndocrinologia
650 $aEstrógeno
650 $aOvino
650 $aPrenhez
650 $aReprodução Animal
650 $aÚtero
700 1 $aMASSMANN, G. A.
700 1 $aMIRABILE, C. P.
700 1 $aFIGUEROA, J. P.
773 $tBiology of Reproduction$gv. 60, n. 5, p. 1198-1203, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
20/12/2012 |
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Data da última atualização: |
09/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SOUZA, G. N. de; MARINHO, L. L.; SILVA, M. R.; GUERRA, U. C.; BRUNO, A. F. |
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Afiliação: |
GUILHERME NUNES DE SOUZA, CNPGL; LILIAN LOPES MARINHO, UFJF; MARCIO ROBERTO SILVA, CNPGL; UESLEI CAMPOS GUERRA, UFV; ANDREA FREGUGLIA BRUNO, UFV. |
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Título: |
Effects of storage temperature and milk sample age on the somatic cell count of goat milk. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Revista do Instituto de Laticinios Cândido Tostes, v. 67, n. 385, p. 38-41, 2012. |
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DOI: |
http://dx.doi.org/10.5935/2238-6416.20120021 |
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Idioma: |
Inglês |
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Conteúdo: |
A temperatura e o tempo de armazenamento de amostras de leite de cabra têm sido identificados como possíveis fatores que influenciam na contagem de células somáticas (CCS). Com objetivo de estabelecer o efeito da temperatura e do tempo de armazenamento de amostras de leite de cabra, foram realizadas em 320 alíquotas de amostras de leite de 20 cabras a CCS utilizando o equipamento Somacount 300 (Bentley Instruments). Foi adicionado o leite foram 5°, 10°, 20° e 30°C e analisadas com 1, 3, 5 e 7 dias após a coleta. A temperatura e tempo de armazenamento não influenciaram significativamente a CCS (P>0,05). Os resultados de CCS em amostras de leite armazenadas a 10°, 20° e 30°C foram sensivelmente menor em relação as amostras armazenadas a 5°C. Considerando uma variação menor de 9%, os resultados da CCS em condições experimentais podem ser aceitos para monitoramento da qualidade do leite e saúde da glândula mamária em rebanhos caprinos leiteiros. |
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Palavras-Chave: |
Amostras de leite; Contagem de células somáticas; Temperatura e tempo de armazenamento. |
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Thesagro: |
Leite de Cabra. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01753naa a2200229 a 4500
001 1943258
005 2024-02-09
008 2012 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.5935/2238-6416.20120021$2DOI
100 1 $aSOUZA, G. N. de
245 $aEffects of storage temperature and milk sample age on the somatic cell count of goat milk.$h[electronic resource]
260 $c2012
520 $aA temperatura e o tempo de armazenamento de amostras de leite de cabra têm sido identificados como possíveis fatores que influenciam na contagem de células somáticas (CCS). Com objetivo de estabelecer o efeito da temperatura e do tempo de armazenamento de amostras de leite de cabra, foram realizadas em 320 alíquotas de amostras de leite de 20 cabras a CCS utilizando o equipamento Somacount 300 (Bentley Instruments). Foi adicionado o leite foram 5°, 10°, 20° e 30°C e analisadas com 1, 3, 5 e 7 dias após a coleta. A temperatura e tempo de armazenamento não influenciaram significativamente a CCS (P>0,05). Os resultados de CCS em amostras de leite armazenadas a 10°, 20° e 30°C foram sensivelmente menor em relação as amostras armazenadas a 5°C. Considerando uma variação menor de 9%, os resultados da CCS em condições experimentais podem ser aceitos para monitoramento da qualidade do leite e saúde da glândula mamária em rebanhos caprinos leiteiros.
650 $aLeite de Cabra
653 $aAmostras de leite
653 $aContagem de células somáticas
653 $aTemperatura e tempo de armazenamento
700 1 $aMARINHO, L. L.
700 1 $aSILVA, M. R.
700 1 $aGUERRA, U. C.
700 1 $aBRUNO, A. F.
773 $tRevista do Instituto de Laticinios Cândido Tostes$gv. 67, n. 385, p. 38-41, 2012.
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Embrapa Gado de Leite (CNPGL) |
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