Data/hora: 02/06/2024 01:32:06

Biblioteca(s): 	Embrapa Uva e Vinho.	
Data corrente: 	10/01/2019	
Data da última atualização: 	09/12/2019	
Autoria: 	POLESI, L. G.; FRAGA, H. P. F. de; VIEIRA, L. do N.; HERINGER, A. S.; ORNELLAS, T. S.; SANTOS, H. P. dos; GUERRA, M. P.; PESCADOR, R.	
Afiliação: 	Luiza Giacomolli Polesi, Graduate Program in Plant Genetic Resources, Laboratory of Plant Developmental Physiology and Genetics, Federal University of Santa Catarina, Florianópolis, SC 88034-001, Brazil; Hugo Pacheco de Freitas Fraga, Graduate Program in Botany, Laboratory of Plant Micropropagation, Federal University of Paraná, Curitiba, PR 81531-980, Brazil; Leila do Nascimento Vieira, Graduate Program in Plant Genetic Resources, Laboratory of Plant Developmental Physiology and Genetics, Federal University of Santa Catarina, Florianópolis, SC 88034-001, Brazil; Angelo Schuabb Heringer, Graduate Program in Plant Genetic Resources, Laboratory of Plant Developmental Physiology and Genetics, Federal University of Santa Catarina, Florianópolis, SC 88034-001, Brazil; Thiago Sanches Ornellas, Graduate Program in Plant Genetic Resources, Laboratory of Plant Developmental Physiology and Genetics, Federal University of Santa Catarina, Florianópolis, SC 88034-001, Brazil; HENRIQUE PESSOA DOS SANTOS, CNPUV; Miguel Pedro Guerra, Tree and Timber Institute, National Research Council of Italy, Via Madonna del Piano 10, Sesto Fiorentino, 50019 Florence, Italy; Rosete Pescador, Graduate Program in Plant Genetic Resources, Laboratory of Plant Developmental Physiology and Genetics, Federal University of Santa Catarina, Florianópolis, SC 88034-001, Brazil.	
Título: 	Chloroplast ultrastructure and hormone endogenous levels are differently affected under light and dark conditions during in vitro culture of Guadua chacoensis (Rojas) Londoño & P. M. Peterson.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	Acta Physiologiae Plantarum , v. 41, n. 10, p. 1-12, Jan. 2019.	
DOI: 	https://doi.org/10.1007/s11738-018-2804-7	
Idioma: 	Inglês	
Conteúdo: 	Guadua chacoensis (Poaceae) is a woody bamboo native from the Atlantic forest biome. Morphogenetic and physiological studies are scarce in bamboos, and tissue culture-based biotechnologies tools can be used to investigate ultrastructure and physiological processes as well as to mass-propagate specific genotypes. This study evaluated the effect of light and dark conditions on chloroplast biogenesis as well as in the endogenous levels of zeatin (Z), abscisic acid (ABA), gibberellic acid (GA4), and jasmonic acid (JA) during in vitro culture of G. chacoensis. An increase was observed, followed by a decrease in starch content in response to light treatment, and in contrast, in darkness, an accumulation of starch which is associated to amyloplast formation at day 30 was observed. No etioplast formation was observed even in the dark and this was associated with the presence of fully developed chloroplast at the beginning of the experiment. Z levels quantified showed distinct behavior, as in light, no difference in the levels was observed, except at day 10, and in darkness, the levels increased along the evaluation time. ABA, GA4, and JA biosynthesis increased along the time of evaluation in light condition. In contrast, in darkness, the levels remained unaltered, excepted for an increase in JA levels at day 10, suggesting a strong relationship between light and these phytohormone levels. Possible reasons why light’s presence and absence cause changes in chloroplast ultrastructure and hormone endogenous levels in in vitro cultivated culms of G. chacoensis are discussed. Keywords Bamboos · Micropropagation · Transmission electron microscopy · Plant physiology · Phytohormone	
Palavras-Chave: 	Atlantic forest biome; Guadua chacoensis (Poaceae); Phytohormone; Woody bamboo native.	
Thesaurus Nal: 	Bamboos; Micropropagation; Plant physiology; Transmission electron microscopy.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/193649/1/Polesi2019-Article-ChloroplastUltrastructureAndHo.pdf	
Registro original: 	Embrapa Uva e Vinho (CNPUV)	
Exemplares: 	CNPUV - (18109-1) - UPC - AP - DD -  -  -  - DIS