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| Registros recuperados : 81 | |
| 21. |  | PAULA, D. P.; TOGAWA, R. C.; COSTA, M. M. C.; GRYNBERG, P.; MARTINS, N. F.; ANDOW, D. A. Identification and expression profile of odorant-binding proteins in Halyomorpha halys (Hemiptera: Pentatomidae). Insect Molecular Biology, v. 25, n. 5, p. 580-594, 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 23. | | CLIFFORD, V. de A.; COSTA, R. M. de J.; TOGAWA, R. C.; QUEIROZ, P. R. M.; GRYNBERG, P. Bacillus amyloliquefaciens group in silico metabolomic characterization and identification of species-specific genomic features eligible for HIGH-resolution QRT-PCR primer design. In: BRAZILIAN SYMPOSIUM ON BIOINFOMATICS (BSB 2023), 16., 2023, Curitiba, PR. [Proceedings...]. Porto Alegre, RS: Sociedade Brasileira de Computação, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 26. | | ARAÚJO, A. C. de; BRASILEIRO, A. C. M.; GUIMARAES, P. M.; GRYNBERG, P.; TOGAWA, R. C.; MOTA, A. Differential gene expression analysis of Arabidopsis thaliana overexpressing expansin AdEXLB8 from Arachis duranensis. In: BRAZILIAN SYMPOSIUM ON BIOINFOMATICS (BSB 2023), 16., 2023, Curitiba, PR. [Proceedings...]. Porto Alegre, RS: Sociedade Brasileira de Computação, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 28. | | GRYNBERG, P.; GUIMARÃES, L. A.; COSTA, M. M. C.; TOGAWA, R. C.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Comprehensive profiling and characterization of Arachis stenosperma (peanut) and Meloidogyne arenaria (plantroot nematode) smallRNAs identified during the course of the infection. In: INTERNATIONAL CONFERENCE OF THE AB3C, 12., 2016, Belo Horizonte. [Proceedings...] [S.l.]: AB3C, 2016. X-meeting 2016.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 29. | | GRYNBERG, P.; GUIMARÃES, L. A.; COSTA, M. M. do C.; TOGAWA, R. C.; BRASILEIRO, A. C. M.; GUIMARAES, P. M. Comprehensive profiling and characterization of Arachis stenosperma (peanut) and Meloidogyne arenaria (plant-root nematode) small-RNAs identified during the course of the infection. In: INTERNATIONAL CONFERENCE OF THE AB3C, 13., 2017, São Pedro. Proceedings... [S.l.]: AB3C, 2017. p. 170| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 30. | | COSTA, R. M. de J.; PURCENA, T.; DANTAS, A. F.; TOGAWA, R. C.; GIMENES, M. A.; GRYNBERG, P. In silico identification and evaluation of tissue expression of possible long lived bean mRNAs through comparative genomic analysis. In: BRAZILIAN SYMPOSIUM ON BIOINFOMATICS (BSB 2023), 16., 2023, Curitiba, PR. [Proceedings...]. Porto Alegre, RS: Sociedade Brasileira de Computação, 2023. Na publicação: Marcos Gimenes.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 31. | | SALGADO, F. F.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Prediction and annotation of miRNAs and the preponderant role of transcription factors in the response of Elaeis guineensis Jacq to abiotic stresses of drought and salinity. In: BRAZILIAN SYMPOSIUM ON BIOINFOMATICS (BSB 2023), 16., 2023, Curitiba, PR. [Proceedings...]. Porto Alegre, RS: Sociedade Brasileira de Computação, 2023.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 32. | | MOURÃO, M. M.; BITAR, M. L.; LOBO, F. P.; GRYNBERG, P.; BORONI, M.; WAISBERG, M.; MACEDO, A. M.; MACHADO, C. R.; FRANCO, G. R. Revealing new perspectives on the regulation of gene expression by the trans-splicing mechanism in Shistosoma mansoni. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. Não paginado. X-MEETING 2011.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 33. | | BEVITORI, R.; TOGAWA, R. C.; COSTA, M. M.; GRYNBERG, P.; GUIMARÃES, L. S. M.; ABREU, A. G. de; MELLO, R.; GROSSI DE SÁ, M. F. Transcriptome profiling of twonear-isogenic rice lines infected by Magnaporthe oryzae using RNA-SEQ. In: CONFERENCIA INTERNACIONAL DO ARROZ PARA AMÉRICA LATINA E CARIBE, 12., 2015, Porto Alegre. Horizonte para a competitividade: anais. Porto Alegre: IRGA, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 34. | | BEVITORI, R.; TOGAWA, R. C.; COSTA, M. M.; GRYNBERG, P.; GUIMARÃES, L. S. M.; ABREU, A. G. de; MELLO, R.; GROSSI DE SÁ, M. F. Transcriptome profiling of twonear-isogenic rice lines infected by Magnaporthe oryzae using RNA-SEQ. In: CONFERENCIA INTERNACIONAL DO ARROZ PARA AMÉRICA LATINA E CARIBE, 12., 2015, Porto Alegre. Horizonte para a competitividade: anais. Porto Alegre: IRGA, 2015.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 35. | | BERÇOT, M. R.; QUEIROZ, P. R. M.; GRYNBERG, P.; TOGAWA, R. C.; MARTINS, E. S.; ROCHA, G. T.; MONNERAT, R. G. Distribution and genetic diversity of genes from Brazilian Bacillus thuringiensis strains toxic to agricultural insect pests revealed by real-time PCR. Microbial Ecology, v. 86, 2515-2526, 2023. Na publicação: Roberto Togawa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 36. | | ALMEIDA, R. F.; SANTOS, I. R.; MEIRA, F. S.; GRYNBERG, P.; LOPES, R.; CUNHA, R. N. V. da; FRANCO, O. L.; SCHERWINSKI-PEREIRA, J. E.; MEHTA, A. Differential protein profiles in interspecific hybrids between Elaeis oleifera and E. guineensis with contrasting responses to somatic embryogenesis competence acquisition. Plant Cell, Tissue and Organ Culture, v. 137, p. 11-21, 2019.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 37. | | CRAVEIRO, S. R.; SANTOS, L. A. V. M.; TOGAWA, R. C.; INGLIS, P. W.; GRYNBERG, P.; RIBEIRO, Z. M. A.; RIBEIRO, B. M.; CASTRO, M. E. B. Complete genome sequences of six Chrysodeixis includens Nucleopolyhedrovirus isolates from Brazil and Guatemala. Genome Announcements, v. 4, n. 6, 2016. e01192-16.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 39. | | VIDAL, L. de A.; GRYNBERG, P.; PETITOT, A.-S.; MOTA, A. P. Z.; TOGAWA, R. C.; FERNANDEZ, D.; FREIRE, E. V. S. A. Validação de sequências candidatas de silenciamento gênico de Meloidogyne Incógnita. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 40. | | SALGADO, F. F.; VIEIRA, L. R.; SILVA, V, N. B.; LEAO, A. P.; GRYNBERG, P.; COSTA, M. M. do C.; TOGAWA, R. C.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Prediction and identification of miRNAS in Elaeis Guinensis Jacq and analysis of their expression in oil palm plants under salinity drought stress. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. p. 275| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 81 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Uva e Vinho. |
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Data corrente: |
14/03/2024 |
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Data da última atualização: |
14/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
FAJARDO, T. V. M.; GRYNBERG, P.; TOGAWA, R. C.; SILVA, J. M. F.; SILVA, F. N. da; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; PRISCILA GRYNBERG, Cenargen; ROBERTO COITI TOGAWA, Cenargen; JOÃO MARCOS FAGUNDES SILVA, INSTITUTE FOR INTEGRATIVE SYSTEMS BIOLOGY; FABIO NASCIMENTO DA SILVA, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; OSMAR NICKEL, CNPUV. |
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Título: |
Analyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Tropical Plant Pathology, [online], March 2024. |
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DOI: |
https://doi.org/10.1007/s40858-024-00647-3 |
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Idioma: |
Inglês |
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Conteúdo: |
The long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses. MenosThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps... Mostrar Tudo |
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Palavras-Chave: |
High throughput sequencing; Vector. |
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Thesaurus NAL: |
Ampelovirus; Closterovirus; Vitivirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162846/1/s40858-024-00647-3.pdf
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Marc: |
LEADER 02312naa a2200253 a 4500
001 2162846
005 2024-03-14
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-024-00647-3$2DOI
100 1 $aFAJARDO, T. V. M.
245 $aAnalyzes of mealybug (Pseudococcus longispinus) virome reveal grapevine viruses diversity.$h[electronic resource]
260 $c2024
520 $aThe long-tailed mealybug, Pseudococcus longispinus, is an important insect pest in grapevine growing areas in several countries, including Brazil. Metagenomic analysis of nucleic acids extracted from insect vectors makes it possible to study the diversity of insect viruses in addition to plant pathogenic viruses. In this study, insects (Ps. longispinus) were collected, and pooled throughout a plot of virus disease symptomatic vines, cultivated in growing beds, and analyzed by high throughput sequencing (HTS). The complete genome of grapevine leafroll-associated virus 2 and 3 (GLRaV-2 and -3) and a partial sequence of grapevine virus A (GVA) with two complete ORFs (coat protein and RNA-binding protein) were assembled from mealybug extracts and exhibited high nucleotide identities, up to 99%, with previously characterized homologous Brazilian isolates from grapevines. This information was validated by the detection of these viruses in the original symptomatic vines (N=76), from where mealybugs were collected, equivalent to an incidence of 34.2%, 89.5% and 36.8% for GLRaV-2, GLRaV-3 and GVA, respectively. Although one of these viruses is not transmitted by mealybugs (GLRaV-2), prospective of plant viruses infecting grapevine plants by analyzing the metagenome of insects could represent a relevant alternative to improve monitoring of viral diseases aiming at the management and control of viral diseases in vineyards or cultivation fields. This work is the first analysis of the Ps. longispinus virome in Brazil focusing on grapevine viruses.
650 $aAmpelovirus
650 $aClosterovirus
650 $aVitivirus
653 $aHigh throughput sequencing
653 $aVector
700 1 $aGRYNBERG, P.
700 1 $aTOGAWA, R. C.
700 1 $aSILVA, J. M. F.
700 1 $aSILVA, F. N. da
700 1 $aNICKEL, O.
773 $tTropical Plant Pathology, [online], March 2024.
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Embrapa Uva e Vinho (CNPUV) |
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