|
|
Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
30/11/2020 |
Data da última atualização: |
01/12/2020 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
BIANCHINI, F.; BIANCHINI, P. C.; BARRETO, R. M. F.; CUNHA, P. A. F. da. |
Afiliação: |
FABRICIO BIANCHINI, CPATSA; PAOLA CORTEZ BIANCHINI, CPATSA; Rebeca Mascarenhas Fonseca Barreto; Paulo Anchieta Florentino da Cunha. |
Título: |
Manejo da agrobiodiversidade em sistema agrícola tradicional fundo de pasto: comunidade de Ouricuri, Uauá-BA. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
In: SILVA, N. C. de A.; COSTA, F. M.; VIDAL, R.; VEASEY, E. A. (Org.). Milhos das terras baixas América do Sul e conservação da agrobiodiversidade no Brasil e no Uruguai. Ponta Grossa: Atena. 2020. |
Páginas: |
cap. 14, p. 195-223. |
Idioma: |
Português |
Conteúdo: |
Este capítulo aborda os sistemas tradicionais de uso, manejo e conservação da biodiversidade e da agrobiodiversidade, partindo de um processo de autodemarcação realizado por meio do mapeamento agroecológico, realizado no ano de 2018 com a Comunidade Tradicional Fundo de Pasto Ouricuri, de Uauá/BA2. A sistematização e discussão dos conhecimentos da comunidade, a torna co-autora deste capítulo. Apresentamos nos resultados a leitura que a comunidade faz do seu território. |
Palavras-Chave: |
Agroecologia; Desenvolvimento territorial; Fundo de pasto; Ouricuri PE; Sistema agricola. |
Thesagro: |
Biodiversidade; Caatinga; Desenvolvimento Rural. |
Thesaurus Nal: |
Biodiversity; Rural communities; Rural development. |
Categoria do assunto: |
B Sociologia Rural |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218478/1/Manejo-da-agrobiodiversidade-em-sistema-agricola-tradicional-fundo-de-pasto-2020.pdf
|
Marc: |
LEADER 01538naa a2200301 a 4500 001 2127348 005 2020-12-01 008 2020 bl uuuu u00u1 u #d 100 1 $aBIANCHINI, F. 245 $aManejo da agrobiodiversidade em sistema agrícola tradicional fundo de pasto$bcomunidade de Ouricuri, Uauá-BA.$h[electronic resource] 260 $c2020 300 $acap. 14, p. 195-223. 520 $aEste capítulo aborda os sistemas tradicionais de uso, manejo e conservação da biodiversidade e da agrobiodiversidade, partindo de um processo de autodemarcação realizado por meio do mapeamento agroecológico, realizado no ano de 2018 com a Comunidade Tradicional Fundo de Pasto Ouricuri, de Uauá/BA2. A sistematização e discussão dos conhecimentos da comunidade, a torna co-autora deste capítulo. Apresentamos nos resultados a leitura que a comunidade faz do seu território. 650 $aBiodiversity 650 $aRural communities 650 $aRural development 650 $aBiodiversidade 650 $aCaatinga 650 $aDesenvolvimento Rural 653 $aAgroecologia 653 $aDesenvolvimento territorial 653 $aFundo de pasto 653 $aOuricuri PE 653 $aSistema agricola 700 1 $aBIANCHINI, P. C. 700 1 $aBARRETO, R. M. F. 700 1 $aCUNHA, P. A. F. da 773 $tIn: SILVA, N. C. de A.; COSTA, F. M.; VIDAL, R.; VEASEY, E. A. (Org.). Milhos das terras baixas América do Sul e conservação da agrobiodiversidade no Brasil e no Uruguai. Ponta Grossa: Atena. 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
29/05/2023 |
Data da última atualização: |
23/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MOREIRA, V. J. V.; PINHEIRO, D. H.; LOURENCO, I. T.; BASSO, M. F.; LISEI-DE-SA, M. E.; SILVA, M. C. M. da; DANCHIN, E. G. J.; GUIMARAES, P. M.; GRYNBERG, P.; BRASILEIRO, A. C. M.; MACEDO, L. L. P. de; MORGANTE, C. V.; ENGLER, J. de A.; SA, M. F. G. de. |
Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, Biotechnology Center, PPGBCM, UFRGS; DANIELE HELOÍSA PINHEIRO, National Institute of Science and Technology; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology; MARIA E. LISEI-DE-SA, National Institute of Science and Technology; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ETIENNE G. J. DANCHIN, National Institute of Science and Technology; PATRICIA MESSEMBERG GUIMARAES, Cenargen; PRISCILA GRYNBERG, Cenargen; ANA CRISTINA MIRANDA BRASILEIRO, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Journal of Pest Science, v. 97, p. 411-427, 2024. |
DOI: |
https://doi.org/10.1007/s10340-023-01623-7 |
Idioma: |
Inglês |
Conteúdo: |
Meloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. MenosMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disor... Mostrar Tudo |
Palavras-Chave: |
DsRNA; Host-induced gene silencing; Interações planta-nematóide; Nematoide das galhas; Plant-nematode interactions; Root-knot nematode. |
Thesagro: |
Meloidogyne Incognita; Nematóide; Parasito de Planta; Planta. |
Thesaurus NAL: |
Nematode biology; Nematode control; Plant parasitic nematodes. |
Categoria do assunto: |
-- O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/264479/1/In-planta-RNAi-targeting-Meloidogyne-incognita-Minc16803-gene-perturbs-nematode-parasitism-and-reduces-plant-susceptibility.pdf
|
Marc: |
LEADER 03269naa a2200445 a 4500 001 2154064 005 2024-05-23 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s10340-023-01623-7$2DOI 100 1 $aMOREIRA, V. J. V. 245 $aIn planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility.$h[electronic resource] 260 $c2024 520 $aMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. 650 $aNematode biology 650 $aNematode control 650 $aPlant parasitic nematodes 650 $aMeloidogyne Incognita 650 $aNematóide 650 $aParasito de Planta 650 $aPlanta 653 $aDsRNA 653 $aHost-induced gene silencing 653 $aInterações planta-nematóide 653 $aNematoide das galhas 653 $aPlant-nematode interactions 653 $aRoot-knot nematode 700 1 $aPINHEIRO, D. H. 700 1 $aLOURENCO, I. T. 700 1 $aBASSO, M. F. 700 1 $aLISEI-DE-SA, M. E. 700 1 $aSILVA, M. C. M. da 700 1 $aDANCHIN, E. G. J. 700 1 $aGUIMARAES, P. M. 700 1 $aGRYNBERG, P. 700 1 $aBRASILEIRO, A. C. M. 700 1 $aMACEDO, L. L. P. de 700 1 $aMORGANTE, C. V. 700 1 $aENGLER, J. de A. 700 1 $aSA, M. F. G. de 773 $tJournal of Pest Science$gv. 97, p. 411-427, 2024.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|