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| Registros recuperados : 383 | |
| 9. |  | NAKASU, E. Y. T.; FIRMINO, A. A. P.; DIAS, S. C.; GROSSI-de-SÁ, M. F. Identificação de receptores do bicudo do algodoeiro (Anthonomus grandis) para toxinas cry. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 71.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | PALÁCIO, T. C.; BARROS, E. V. S. A.; GROSSI-de-SÁ, M. F.; TEIXEIRA, J. B. Indução de calos embriogênicos em explantes foliares de Coffea canephora submetidos a diferentes tratamentos com 2,4-Diclorofenoxiacético (2,4-D). In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 49.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | SILVA, T.; VASCONCELOS, É. A. R.; ROCHA, T. L.; GROSSI-de-SÁ, M. F. Atividade quitinásica em extratos protéicos de Bacillus thuringiensis tóxicos para o bicudo do algodoeiro (Anthonomus grandis). In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 53.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | ARBOLEDA, V. J. W.; JIMÉNEZ, A. V.; SILVA, M. S.; GROSSI DE SÁ, M. F. Caracterização parcial de enzimas proteolíticas intestinais de larvas de broca gigante da cana, Castnia licus (Drury). In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 43-48. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | ÁTILA-SANTOS, D.; MULINARI, F.; CHRISPEELS, M. J.; GROSSI-DE-SÁ, M. F. Alpha-amilase de Acanthoscelides obtectus: clonagem e expressão heteróloga em Pichia pastoris. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 57.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | VASCONCELOS, E. A. R.; SILVA, T. S.; ROCHA, T. L.; GROSSI DE SÁ, M. F. 2D-E reference maps of celullar and secreted proteins from Bacillus thuringiensis (S811) during growth and sporulation. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
01/09/2020 |
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Data da última atualização: |
19/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. |
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Afiliação: |
THUANNE PIRES RIBEIRO, UNB; MARCOS FERNANDO BASSO; MAYARA HOLANDA DE CARVALHO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; DAGNA MARIA LAURINDO DA SILVA; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OSMUNDO BRILANTE DE OLIVEIRA-NETO; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; BRUNA MENDES DINIZ TRIPODE, CNPA; TATIANNE PIZA FERRARI ABREU-JARDIM; JOSE EDNILSON MIRANDA, CNPA; MARCIO ALVES-FERREIRA, UFRJ; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 3, p. 27-41, 2020. |
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DOI: |
https://doi.org/10.1016/j.biori.2019.12.003 |
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Idioma: |
Inglês |
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Conteúdo: |
The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW. MenosThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events ... Mostrar Tudo |
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Palavras-Chave: |
Bacillus; Bicudo do algodão; Entomotoxin; Herança estável; Insect pest; Management; Praga de inseto; Resistance; Resistência gestão; Stable inheritance; Thuringiensis. |
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Thesagro: |
Algodão; Inseto; Praga; Praga de Planta. |
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Thesaurus NAL: |
Cotton; Insect pests; Resistance management. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216823/1/Stability-and-tissue-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215669/1/1-s2.0-S2452072119301352-main.pdf
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Marc: |
LEADER 03317naa a2200529 a 4500
001 2125667
005 2020-11-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.biori.2019.12.003$2DOI
100 1 $aRIBEIRO, T. P.
245 $aStability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil.$h[electronic resource]
260 $c2020
520 $aThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW.
650 $aCotton
650 $aInsect pests
650 $aResistance management
650 $aAlgodão
650 $aInseto
650 $aPraga
650 $aPraga de Planta
653 $aBacillus
653 $aBicudo do algodão
653 $aEntomotoxin
653 $aHerança estável
653 $aInsect pest
653 $aManagement
653 $aPraga de inseto
653 $aResistance
653 $aResistência gestão
653 $aStable inheritance
653 $aThuringiensis
700 1 $aBASSO, M. F.
700 1 $aCARVALHO, M. H. de
700 1 $aMACEDO, L. L. P. de
700 1 $aSILVA, D. M. L. da
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aOLIVEIRA-NETO, O. B. de
700 1 $aCAMPOS-PINTO, E. R. de
700 1 $aLUCENA, W. A.
700 1 $aSILVA, M. C. M. da
700 1 $aTRIPODE, B. M. D.
700 1 $aABREU-JARDIM, T. P. F.
700 1 $aMIRANDA, J. E.
700 1 $aALVES-FERREIRA, M.
700 1 $aMORGANTE, C. V.
700 1 $aGROSSI-DE-SA, M. F.
773 $tBiotechnology Research and Innovation$gv. 3, p. 27-41, 2020.
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Embrapa Semiárido (CPATSA) |
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