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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
26/07/2004 |
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Data da última atualização: |
18/06/2021 |
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Autoria: |
ALENCAR, M. M. de; PACKER, I. U.; RAZOOK, A. G.; FIGUEIREDO, L. A.; BARBOSA, P. F.; CORRÊA, L. de A. |
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Título: |
Análises de características produtivas em diferentes sistemas de cruzamento entre raças bovinas de corte. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 41., 2004, Campo Grande, MS. Anais... Campo Grande: SBZ, 2004. |
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Páginas: |
4f. |
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Descrição Física: |
1CD-ROM. |
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Idioma: |
Português |
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Thesagro: |
Cruzamento Animal; Gado de Corte. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/15171/1/PROCIMMA2004.00002.pdf
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Marc: |
LEADER 00692nam a2200193 a 4500
001 1045576
005 2021-06-18
008 2004 bl uuuu u00u1 u #d
100 1 $aALENCAR, M. M. de
245 $aAnálises de características produtivas em diferentes sistemas de cruzamento entre raças bovinas de corte.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 41., 2004, Campo Grande, MS. Anais... Campo Grande: SBZ$c2004
300 $a4f.$c1CD-ROM.
650 $aCruzamento Animal
650 $aGado de Corte
700 1 $aPACKER, I. U.
700 1 $aRAZOOK, A. G.
700 1 $aFIGUEIREDO, L. A.
700 1 $aBARBOSA, P. F.
700 1 $aCORRÊA, L. de A.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
14/01/2011 |
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Data da última atualização: |
17/04/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VASCONCELOS, E. A. R.; SANTANA, C. G.; GODOY, C. V.; SILVA, M. S.; MOREIRA, L. R. S.; OLIVEIRA-NETO, OSMUNDO B.; PRICE, D.; FITCHES, E.; FILHO, E. X. F.; MEHTA, A.; GATEHOUSE, J. A.; GROSSI-DE-SA, M. F. |
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Afiliação: |
ÉRICO A. R. VASCONCELOS, UNB; CELSO G. SANTANA; CLAUDIA VIEIRA GODOY, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO X. F. FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control. |
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Palavras-Chave: |
Asian rust. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/31109/1/cbb.errata.cvg.pdf
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Marc: |
LEADER 02303nam a2200253 a 4500
001 1873147
005 2018-04-17
008 2010 bl uuuu u00u1 u #d
100 1 $aVASCONCELOS, E. A. R.
245 $aA new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
260 $aIn: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. [Programa e resumos]: errata. Brasília, DF: SBBiotec$c2010
520 $aAsian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. In order to find a biochemical candidate to such enterprise, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (ß/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay using purified protein showed that CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at sub-molar equivalence (12:1 enzyme:inhibitor ratio). Additionally, CaclXIP at 1.5 ?g/?L inhibited by 45% the germination of spores of Phakopsora pachyrhizi. These results show that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes of it an eligible candidate gene for Asian rust control.
653 $aAsian rust
700 1 $aSANTANA, C. G.
700 1 $aGODOY, C. V.
700 1 $aSILVA, M. S.
700 1 $aMOREIRA, L. R. S.
700 1 $aOLIVEIRA-NETO, OSMUNDO B.
700 1 $aPRICE, D.
700 1 $aFITCHES, E.
700 1 $aFILHO, E. X. F.
700 1 $aMEHTA, A.
700 1 $aGATEHOUSE, J. A.
700 1 $aGROSSI-DE-SA, M. F.
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Embrapa Soja (CNPSO) |
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