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| Registros recuperados : 400 | |
| 7. |  | SOUZA, D. S. L.; BARBOSA, A. E. A. D.; GROSSI-de-SÁ, M.; CAVALCANTE, W. S.; BARROS, E. V. S. A.; GROSSI-de-SÁ, M. F. Identificação de potenciais genes de resistência a fitonematóides presentes no genoma do café. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 67.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | SOUZA, D. S. L.; GROSSI-DE-SÁ, M.; ROMANO, E.; TEIXEIRA, F. M.; PIRES, N.; ROCHA, T. L.; BARBOSA, A. E. A. D.; GROSSI-DE-SÁ, M. F. Análise funcional de genes envolvidos com fitoparasitismo de Meloidogyne incognita por silenciamento e superexpressão em plantas transgênicas de genes específicos de glândulas esofagianas. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 61.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | GROSSI-DE-SÁ, M.; SOUZA, D. S. L.; ROMANO, E.; TEIXEIRA, F. M.; ARAÚJO, B.; ROCHA, T. L.; BARBOSA, A. E. A. D.; GROSSI-DE-SÁ, M. F. Análise funcional de genes envolvidos com fitoparasitismo em Meloidogyne incognita por soaking RNAi. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 62.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | MULINARI, F.; STANISÇUASKI, F.; CARLINI, C. R.; GROSSI-de-SÁ, M. F. Jaburetox-2ec: peptídeo recombinante derivado de urease de canavalia ensiformis com atividade inseticida. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 9., 2004, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2004. p. 70.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | FRAGOSO, R. R.; BATISTA, J. A. N.; OLIVEIRA-NETO, O. B.; GROSSI-de-SÁ, M. F. Isolamento e caracterização de cDNAs codificadores de proteases serina e aspartil de nematóide formador de galhas Meloidogyne incógnita. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 35.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | LOURENÇO, I. T.; FRAGOSO, R. R.; SOUZA JÚNIOR, A. J. D.; GROSSI-DE-SÁ, M. F. Isolamento de genes e construção de vetores para uso no silenciamento gênico de Meloidogyne incognita. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 041. p. 81.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | LOURENÇO, I. T.; FRAGOSO, R. R.; SOUZA JÚNIOR, A. J. D.; GROSSI-DE-SÁ, M. F. Isolamento de genes e construção de vetores para uso no silenciamento gênico de Meloidogyne incognita. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008.| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | COSTA, P. M.; FREIRE, E. V. S. A.; GUIMARÃES, L. M.; GROSSI-de-SÁ, M. F. Análise da expressão do gene GUS em diferentes eventos de transformação de Coffea arabica cv. catuaí vermelho. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 39.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | VASCONCELOS, E. A. R.; SILVA, T. S.; ROCHA, T. L.; GROSSI DE SÁ, M. F. 2D-E reference maps of celullar and secreted proteins from Bacillus thuringiensis (S811) during growth and sporulation. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | ÁTILA-SANTOS, D.; MULINARI, F.; CHRISPEELS, M. J.; GROSSI-DE-SÁ, M. F. Alpha-amilase de Acanthoscelides obtectus: clonagem e expressão heteróloga em Pichia pastoris. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 57.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | ARBOLEDA, V. J. W.; JIMÉNEZ, A. V.; SILVA, M. S.; GROSSI DE SÁ, M. F. Caracterização parcial de enzimas proteolíticas intestinais de larvas de broca gigante da cana, Castnia licus (Drury). In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 43-48. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 400 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
04/09/2020 |
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Data da última atualização: |
27/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NORIEGA, D. D.; ARRAES, F. B. M.; ANTONINO, J. D.; MACEDO, L. L. P.; FONSECA, F. C. A.; TOGAWA, R. C.; GRYNBERG, P.; SILVA, M. C. M.; NEGRISOLI JUNIOR, A. S.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. |
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Afiliação: |
DANIEL D. NORIEGA, UNB; FABRICIO B. M. ARRAES, UFRGS; JOSÉ DIJAIR ANTONINO, UFRPE; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; FERNANDO C. A. FONSECA, UNB; ROBERTO COITI TOGAWA, Cenargen; PRISCILA GRYNBERG, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ALDOMARIO SANTO NEGRISOLI JUNIOR, CPATC; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Comparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
PLoS ONE, v. 15, n. 8, e0235575, 2020. |
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DOI: |
https://doi.org/10.1371/journal.pone.0235575 |
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Idioma: |
Inglês |
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Conteúdo: |
The sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence, igestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses in hosts and herbivorous insects, the success of phytophagous insects relies on their ability to overcome challenges such as the response to plant defences and the intake of nutrients. In this study, we identified metabolic pathways and specific genes involved in these processes. Thus, our data strongly contribute to the knowledge advancement of insect transcripts, which can be a source of target genes for pest management MenosThe sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence, igestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses... Mostrar Tudo |
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Palavras-Chave: |
Controle biotecnológico; Controle de praga; D saccharalis; Genetic Aspects; Manejo da praga; Physiological Aspects. |
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Thesagro: |
Biotecnologia; Cana de Açúcar; Diatraea Saccharalis; Dieta; Doença; Praga. |
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Thesaurus NAL: |
Biotechnology; Genomics; Pest control; Pest management; Sugarcane; Transcription factors. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215768/1/journal.pone.0235575.pdf
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Marc: |
LEADER 03260naa a2200469 a 4500
001 2128710
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008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0235575$2DOI
100 1 $aNORIEGA, D. D.
245 $aComparative gut transcriptome analysis of Diatraea saccharalis in response to the dietary source.$h[electronic resource]
260 $c2020
520 $aThe sugarcane borer (Diatraea saccharalis, Fabricius, 1794) is a devastating pest that causes millions of dollars of losses each year to sugarcane producers by reducing sugar and ethanol yields. The control of this pest is difficult due to its endophytic behavior and rapid development. Pest management through biotechnological approaches has emerged in recent years as an alternative to currently applied methods. Genetic information about the target pests is often required to perform biotechnology-based management. The genomic and transcriptomic data for D. saccharalis are very limited. Herein, we report a tissue-specific transcriptome of D. saccharalis larvae and a differential expression analysis highlighting the physiological characteristics of this pest in response to two different diets: sugarcane and an artificial diet. Sequencing was performed on the Illumina HiSeq 2000 platform, and a de novo assembly was generated. A total of 27,626 protein-coding unigenes were identified, among which 1,934 sequences were differentially expressed between treatments. Processes such as defence, igestion, detoxification, signaling, and transport were highly represented among the differentially expressed genes (DEGs). Furthermore, seven aminopeptidase genes were identified as candidates to encode receptors of Cry proteins, which are toxins of Bacillus thuringiensis used to control lepidopteran pests. Since plantinsect interactions have produced a considerable number of adaptive responses in hosts and herbivorous insects, the success of phytophagous insects relies on their ability to overcome challenges such as the response to plant defences and the intake of nutrients. In this study, we identified metabolic pathways and specific genes involved in these processes. Thus, our data strongly contribute to the knowledge advancement of insect transcripts, which can be a source of target genes for pest management
650 $aBiotechnology
650 $aGenomics
650 $aPest control
650 $aPest management
650 $aSugarcane
650 $aTranscription factors
650 $aBiotecnologia
650 $aCana de Açúcar
650 $aDiatraea Saccharalis
650 $aDieta
650 $aDoença
650 $aPraga
653 $aControle biotecnológico
653 $aControle de praga
653 $aD saccharalis
653 $aGenetic Aspects
653 $aManejo da praga
653 $aPhysiological Aspects
700 1 $aARRAES, F. B. M.
700 1 $aANTONINO, J. D.
700 1 $aMACEDO, L. L. P.
700 1 $aFONSECA, F. C. A.
700 1 $aTOGAWA, R. C.
700 1 $aGRYNBERG, P.
700 1 $aSILVA, M. C. M.
700 1 $aNEGRISOLI JUNIOR, A. S.
700 1 $aMORGANTE, C. V.
700 1 $aGROSSI-DE-SA, M. F.
773 $tPLoS ONE$gv. 15, n. 8, e0235575, 2020.
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Embrapa Tabuleiros Costeiros (CPATC) |
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