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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
26/12/2017 |
Data da última atualização: |
30/10/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FRANCISCO, R.; BRANCO, R.; SCHWAB, S.; BALDANI, J. I. |
Afiliação: |
ROMEU FRANCISCO, UNIVERSIDADE DE COIMBRA.; RITA BRANCO, UNIVERSIDADE DE COIMBRA; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB. |
Título: |
Impact of plant-associated bacteria biosensors on plant growth in the presence of hexavalent chromium. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 34, n. 12, p. 1-12, 2018. |
ISSN: |
1573-0972 |
DOI: |
https://doi.org/10.1007/s11274-017-2389-0 |
Idioma: |
Inglês |
Conteúdo: |
Cr(VI) is a highly toxic metal produced by anthropogenic activity which may impact the environment, affecting plants and animals. In plants, chromium both as Cr(III) or Cr(VI) can be absorbed by roots, is poorly translocated and affects negatively plant growth. Plants used in phytoremediation need to cope with chromium toxicity. This work aimed to evaluate strains of Ochrobactrum tritici and Nitrospirillum amazonense, resistant and modified in order to become chromate whole-cell biosensors, as plant-protectors enabling plants to withstand contaminated soils. In vitro tests were performed in three rice varieties and one maize variety. Initial evaluations of Cr(VI) toxicity to plants showed that plants had different sensitivities and BRS 6 CHUÍ rice variety was the most resistant. The metal affected plant growth and development, essentially in roots which were totally inhibited in rice varieties at 500 μM. This effect was plant-dependent. Modified N. amazonense proved to protect maize plants independently of the inoculation dose but O. tritici showed plant specificity and some toxicity when inoculated at high numbers, inhibiting rice development but not maize. Inoculants were directly responsible for growth improvements of specific plant varieties at 1.25 ppm Cr(VI), a concentration which corresponds to a weak soil contamination. Improvements were observed relatively to the Cr(VI)-treated controls, but also relative to the untreated controls, i.e., the benefits went beyond a simple neutralization of inhibition brought by Cr(VI) toxicity. MenosCr(VI) is a highly toxic metal produced by anthropogenic activity which may impact the environment, affecting plants and animals. In plants, chromium both as Cr(III) or Cr(VI) can be absorbed by roots, is poorly translocated and affects negatively plant growth. Plants used in phytoremediation need to cope with chromium toxicity. This work aimed to evaluate strains of Ochrobactrum tritici and Nitrospirillum amazonense, resistant and modified in order to become chromate whole-cell biosensors, as plant-protectors enabling plants to withstand contaminated soils. In vitro tests were performed in three rice varieties and one maize variety. Initial evaluations of Cr(VI) toxicity to plants showed that plants had different sensitivities and BRS 6 CHUÍ rice variety was the most resistant. The metal affected plant growth and development, essentially in roots which were totally inhibited in rice varieties at 500 μM. This effect was plant-dependent. Modified N. amazonense proved to protect maize plants independently of the inoculation dose but O. tritici showed plant specificity and some toxicity when inoculated at high numbers, inhibiting rice development but not maize. Inoculants were directly responsible for growth improvements of specific plant varieties at 1.25 ppm Cr(VI), a concentration which corresponds to a weak soil contamination. Improvements were observed relatively to the Cr(VI)-treated controls, but also relative to the untreated controls, i.e., the benefits went beyon... Mostrar Tudo |
Palavras-Chave: |
Chromium PGPB; Itrospirillum amazonence; Maize. |
Thesaurus Nal: |
Ochrobactrum tritici; rice. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02318naa a2200241 a 4500 001 2083597 005 2018-10-30 008 2018 bl uuuu u00u1 u #d 022 $a1573-0972 024 7 $ahttps://doi.org/10.1007/s11274-017-2389-0$2DOI 100 1 $aFRANCISCO, R. 245 $aImpact of plant-associated bacteria biosensors on plant growth in the presence of hexavalent chromium.$h[electronic resource] 260 $c2018 520 $aCr(VI) is a highly toxic metal produced by anthropogenic activity which may impact the environment, affecting plants and animals. In plants, chromium both as Cr(III) or Cr(VI) can be absorbed by roots, is poorly translocated and affects negatively plant growth. Plants used in phytoremediation need to cope with chromium toxicity. This work aimed to evaluate strains of Ochrobactrum tritici and Nitrospirillum amazonense, resistant and modified in order to become chromate whole-cell biosensors, as plant-protectors enabling plants to withstand contaminated soils. In vitro tests were performed in three rice varieties and one maize variety. Initial evaluations of Cr(VI) toxicity to plants showed that plants had different sensitivities and BRS 6 CHUÍ rice variety was the most resistant. The metal affected plant growth and development, essentially in roots which were totally inhibited in rice varieties at 500 μM. This effect was plant-dependent. Modified N. amazonense proved to protect maize plants independently of the inoculation dose but O. tritici showed plant specificity and some toxicity when inoculated at high numbers, inhibiting rice development but not maize. Inoculants were directly responsible for growth improvements of specific plant varieties at 1.25 ppm Cr(VI), a concentration which corresponds to a weak soil contamination. Improvements were observed relatively to the Cr(VI)-treated controls, but also relative to the untreated controls, i.e., the benefits went beyond a simple neutralization of inhibition brought by Cr(VI) toxicity. 650 $aOchrobactrum tritici 650 $arice 653 $aChromium PGPB 653 $aItrospirillum amazonence 653 $aMaize 700 1 $aBRANCO, R. 700 1 $aSCHWAB, S. 700 1 $aBALDANI, J. I. 773 $tWorld Journal of Microbiology and Biotechnology$gv. 34, n. 12, p. 1-12, 2018.
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Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
02/02/2017 |
Data da última atualização: |
16/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ISAZA, R. E. A.; DIAZ-TRUJILLO, C.; DHILLON, B.; AERTS, A.; CARLIER, J.; CRANE, C. F.; JONG, T. V.; VRIES, I.; DIETRICH, R.; FARMER, A. D.; FERREIRA, C. F.; GARCIA, S.; GUZMAN, M.; HAMELIN, R. C.; LINDQUIST, E. A.; MEHRABI, R.; QUIROS, O.; SCHMUTZ, J.; SHAPIRO, H.; REYNOLDS, E.; SCALLIET, G.; SOUZA JUNIOR, M. T.; STERGIOPOULOS, I.; VAN DER LEE, T. A. J.; WIT, P. J. G. M. DE; ZAPATER, M.-F.; ZWIERS, L.-H.; GRIGORIEV, I. V.; GOODWIN, S. B.; KEMA, G. H. J. |
Afiliação: |
RAFAEL E. ARANGO ISAZA; CAUCASELLA DIAZ-TRUJILLO; BRAHAM DHILLON; ANDREA AERTS; JEAN CARLIER; CHARLES F. CRANE; TRISTAN V. DE JONG; INEKE DE VRIES; ROBERT DIETRICH; ANDREW D. FARMER; CLAUDIA FORTES FERREIRA, CNPMF; SUZANA GARCIA; MAURICIO GUZMAN; RICHARD C. HAMELIN; ERIKA A. LINDQUIST; RAHIM MEHRABI; OLMAN QUIROS; JEREMY SCHMUTZ; HARRIS SHAPIRO; ELIZABETH REYNOLDS; GABRIEL SCALLIET; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE; IOANNIS STERGIOPOULOS; THEO A. J. VAN DER LEE; PIERRE J. G. M. DE WIT; MARIE-FRANÇOISE ZAPATER; LUTE-HARM ZWIERS; IGOR V. GRIGORIEV; STEPHEN B. GOODWIN; GERT H. J. KEMA. |
Título: |
Combating a global threat to a clonal crop: banana black sigatoka pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) genomes reveal clues for disease control. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
PLOS Genetics, v. 12, n.10, August, 2016. |
ISSN: |
1553-7404 |
DOI: |
10.1371 |
Idioma: |
Inglês |
Conteúdo: |
Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this pathogen and in improving the efficiency of banana breeding programs. MenosBlack Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosp... Mostrar Tudo |
Palavras-Chave: |
Controle de pragas; Pseudocercospora fijiensis. |
Thesagro: |
Mycosphaerella Fijiensis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03582naa a2200529 a 4500 001 2062516 005 2022-08-16 008 2016 bl uuuu u00u1 u #d 022 $a1553-7404 024 7 $a10.1371$2DOI 100 1 $aISAZA, R. E. A. 245 $aCombating a global threat to a clonal crop$bbanana black sigatoka pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) genomes reveal clues for disease control.$h[electronic resource] 260 $c2016 520 $aBlack Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this pathogen and in improving the efficiency of banana breeding programs. 650 $aMycosphaerella Fijiensis 653 $aControle de pragas 653 $aPseudocercospora fijiensis 700 1 $aDIAZ-TRUJILLO, C. 700 1 $aDHILLON, B. 700 1 $aAERTS, A. 700 1 $aCARLIER, J. 700 1 $aCRANE, C. F. 700 1 $aJONG, T. V. 700 1 $aVRIES, I. 700 1 $aDIETRICH, R. 700 1 $aFARMER, A. D. 700 1 $aFERREIRA, C. F. 700 1 $aGARCIA, S. 700 1 $aGUZMAN, M. 700 1 $aHAMELIN, R. C. 700 1 $aLINDQUIST, E. A. 700 1 $aMEHRABI, R. 700 1 $aQUIROS, O. 700 1 $aSCHMUTZ, J. 700 1 $aSHAPIRO, H. 700 1 $aREYNOLDS, E. 700 1 $aSCALLIET, G. 700 1 $aSOUZA JUNIOR, M. T. 700 1 $aSTERGIOPOULOS, I. 700 1 $aVAN DER LEE, T. A. J. 700 1 $aWIT, P. J. G. M. DE 700 1 $aZAPATER, M.-F. 700 1 $aZWIERS, L.-H. 700 1 $aGRIGORIEV, I. V. 700 1 $aGOODWIN, S. B. 700 1 $aKEMA, G. H. J. 773 $tPLOS Genetics$gv. 12, n.10, August, 2016.
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