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| Registros recuperados : 34 | |
| 2. |  | PADILHA, C. V. da S.; BIASOTO, A. C. T.; CORRÊA, L. C.; LIMA, M. dos S. Phenolic compounds and antioxidant activity of commercial tropical red wines (Vitis vinifera) from the São Francisco Valley, Brazil. IN: INTERNATIONAL SYMPOSIUM ON TROPICAL WINES, 5., 2016, Petrolina, PE. Book of abstracts...Petrolina, PE: Embrapa Semiárido, p. 58, 19 a 21 outubro, 2016.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | PADILHA, C. V. da S.; BIASOTO, A. C. T.; CORREA, L. C.; LIMA, M. dos S.; PEREIRA, G. E. Phenolic compounds and antioxidant activity of commercial tropical red wines (Vitis vinifera) from the São Francisco Valley, Brazil. In: INTERNATIONAL SYMPOSIUM ON TROPICAL WINES, 5., 2016, Petrolina. Book of abstracts... Petrolina: Embrapa Semiárido, 2016. p. 58.| Biblioteca(s): Embrapa Semiárido. |
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| 10. | | COELHO, B. E. S.; LIMA, M. dos S.; RYBKA, A. C. P.; SOUZA, F. de F.; FREITAS, S. T. de. Coloração da polpa de acerola obtida sob diferentes formas de processamento. In: CONGRESSO INTERNACIONAL INTERDISCIPLINAR EM EXTENSÃO RURAL E DESENVOLVIMENTO, 2., 2019, Juazeiro, BA. Territorialidades, mudanças climáticas, agroecossistemas e premissas para o desenvolvimento sustentável. Juazeiro, BA: UNIVASF, 2019.| Biblioteca(s): Embrapa Semiárido. |
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| 14. | | FERREIRA, I. C.; SILVA, V. P. da; VILVERT, J. C.; SOUZA, F. de F.; FREITAS, S. T. de; LIMA, M. dos S. Brazilian varieties of acerola (Malpighia emarginata DC.) produced under tropical semi- arid conditions: Bioactive phenolic compounds, sugars, organic acids, and antioxidant capacity. Journal of Food Biochemistry, v. 45, n. 8, aug. 2021.| Biblioteca(s): Embrapa Semiárido. |
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| 15. | | LIMA, M. dos S.; DUTRA, M. da C. P.; TOALDO, I. M.; CORREA, L. C.; PEREIRA, G. E.; OLIVEIRA, D. de; BORDIGNON-LUIZ, M. T.; NINOW, J. L. Phenolic Compounds, Organic Acids and Antioxidant Activity of Grape Juices Produced in Industrial Scale by Different Processes of Maceration. Food Chemistry, v. 188, p. 384-392, Dec. 2015.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 16. | | DA SILVA, M. J. R.; PADILHA, C. V. da S.; LIMA, M. dos S.; PEREIRA, G. E.; VENTURINI FILHO, W. G.; MOURA, M. F.; TECCHIO, M. A. Grape juices produced from new hybrid varieties grown on Brazilianrootstocks?Bioactive compounds, organic acids and antioxidant capacity. Food Chemistry, v. 289, p. 714-722, 2019.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 18. | | SOUZA, J. F. de; NASCIMENTO, A. M. de S.; LINHARES, M. do S. S.; DUTRA, M. da C. P.; LIMA, M. dos S.; PEREIRA, G. E. Evolution of Phenolic Compound Profiles and Antioxidant Activity of Syrah Red and Sparkling Moscatel Wines Stored in Bottles of Different Colors. Beverages, v. 4, n. 89, p. 1-13, 2018.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 19. | | COELHO, E. M.; PADILHA, C. V. da S.; MISKINIS, G. A.; SÁ, A. G. B. de; PEREIRA, G. E.; AZEVEDO, L. C. de; LIMA, M. dos S. Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil. Journal of Food Composition and Analysis, v. 66, p. 160-167, 2018.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 20. | | SILVA, G. G.; OLIVEIRA, J. B. de; SANTOS, S. de F.; LIMA, M. dos S.; BIASOTO, A. C. T.; PEREIRA, G. E. Sensory evaluation of grape juices elaborated by different extraction methods in the São Francisco Valley, Brazil. IN: INTERNATIONAL SYMPOSIUM ON TROPICAL WINES, 5., 2016, Petrolina. Book of abstracts... Petrolina: Embrapa Semiárido, 2016. p. 38.| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 34 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/05/2022 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AMARAL, T. F.; GRAZIA, J. G. V. de; MARTINHAO, L A. G.; DE COL, F.; SIQUEIRA, L. G. B.; VIANA, J. H. M.; HANSEN, P. J. |
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Afiliação: |
THIAGO F. AMARAL, University of Florida; JOAO GABRIEL VIANA DE GRAZIA, FIVX Apoyar Biotech Ltda; LUANY ALVES GALVAO MARTINHAO, FIVX Apoyar Biotech Ltda; FELIPE DE COL, FIVX Apoyar Biotech Ltda; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; JOAO HENRIQUE MOREIRA VIANA, Cenargen; PETER J. HANSEN, University of Florida. |
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Título: |
Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Scientific Reports, v. 12, 7503, 2022. |
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DOI: |
https://doi.org/10.1038/s41598-022-11447-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Procedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer. MenosProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro w... Mostrar Tudo |
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Palavras-Chave: |
Fertilização; Produção in vitro. |
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Thesagro: |
Bovino; Embrião; Reprodução Animal. |
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Thesaurus NAL: |
Animal reproduction; Embryo (animal); In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142749/1/Actions-of-CSF2.pdf
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Marc: |
LEADER 02687naa a2200301 a 4500
001 2142749
005 2022-05-09
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-022-11447-7$2DOI
100 1 $aAMARAL, T. F.
245 $aActions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium.$h[electronic resource]
260 $c2022
520 $aProcedures for in vitro embryo production in cattle have not been optimized. In the current experiment, we utilized a 3 × 3 factorial design to test whether the proportion of embryos becoming blastocysts in culture and the pregnancy rate after embryo transfer are afected by type of serum in the medium [no serum; 3% (v/v) KnockOut Serum Replacement (SR); 3% (v/v) fetal bovine serum (FBS)] and addition of specifc embryokines [vehicle; 10 ng/mL colony stimulating factor 2 (CSF2); 100 ng/mL dickkopf related protein 1 (DKK1)] at day 5 of culture. Embryos were produced using abattoir-derived ovaries and Y-sorted semen from two Angus sires. The percent of putative zygotes and cleaved embryos becoming blastocysts was improved by SR and FBS. Pregnancy rate at day 30 was determined for 1426 Nelore recipients and calving rate for 266 recipients. In the absence of CSF2 or DKK1, pregnancy rates were lower for embryos cultured with SR or FBS. CSF2 and DKK1 reduced pregnancy rate for embryos cultured without serum but had no detrimental efect in the SR or FBS groups. Indeed, CSF2 blocked the negative efect of FBS on pregnancy rate. Data on birth weights were available for 67 bull calves. There were no efects of treatment. The sire used to produce embryos had signifcant and large efects on development to the blastocyst stage, pregnancy rate at day 30, calving rate and pregnancy loss between day 30 and calving. Results indicate that (1) SR and FBS can improve embryonic development in vitro while also compromising competence of embryos to survive after transfer, (2) actions of CSF2 and DKK1 depend upon other characteristics of the embryo production system, and (3) sire can have a large efect on embryonic development before and after transfer.
650 $aAnimal reproduction
650 $aEmbryo (animal)
650 $aIn vitro fertilization
650 $aBovino
650 $aEmbrião
650 $aReprodução Animal
653 $aFertilização
653 $aProdução in vitro
700 1 $aGRAZIA, J. G. V. de
700 1 $aMARTINHAO, L A. G.
700 1 $aDE COL, F.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aVIANA, J. H. M.
700 1 $aHANSEN, P. J.
773 $tScientific Reports$gv. 12, 7503, 2022.
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Embrapa Gado de Leite (CNPGL) |
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