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| Registros recuperados : 32 | |
| 10. |  | GRAMACHO, K. P.; RISTERUCCI, A. M.; LANAUD, C.; BAHIA, C.; LOPES, U. V.; GESTEIRA, A. da S.; ARAÚJO, M. R. Development of microsatelllte markers for the genetic analysis of Crinipellis perniciosa. Agrotrópica, v. 17, p61 - 64,dez. 2005.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | GOMES, L. I. S.; SILVA, C. A.; LIMA, H. E. de; NEVES, E. D.; GRAMACHO, K. P. Genetic variability of Moniliophthora perniciosa isolates from cupuassu in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. Expofito. Ouro Preto: UFV, 2013.| Biblioteca(s): Embrapa Roraima. |
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| 12. | | GRAMACHO, K. P.; REIS, M. R. A.; PIRES, A. B. L.; LOPES, U. V.; GESTEIRA, A. da S.; RAMADA, M. H. S. Analise in silico de EST-SSRs como ferramenta para estudar a biologia de Moniliophthora perniciosa. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 48.; CONGRESSO BRASILEIRO DE PATOLOGIA PÓS-COLHEITA, 2., 2015, São Pedro. Fitopatologia de precisão: fronteiras da ciência: anais. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | PUNGARTNIK, C.; SILVA, A. C. da; MELO, S. A. de; GRAMACHO, K. P.; CASCARDO, J. C. de M.; BRENDEL, M.; MICHELI, F.; GESTEIRA, A. da S. High-affinity copper transport and Snq2 export permease of Saccharomyces cerevisiae modulate cytotoxicity of PR-10 from Theobroma cacao. Molecular Plant-Microbe Interactions, St. Paul, v. 22, n. 1, p. 39-51, jan. 2009. DOI: 10.1094/MPMI - 22 - 1 - 0039| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | LOPES, M. A.; HORA JUNIOR, B. T.; DIAS, C. V.; SANTOS, G. C.; GRAMACHO, K. P.; CASCARDO, J. C. M.; GESTEIRA, A. da S.; MICHELI, F. Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae). Genetics and Molecualr Reseach, Ribeirão Preto, v. 9, n. 3, p. 1279-1297, 2010.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | RAMADA, M. H. S.; BRAND, G. D.; ABRÃO, F. Y.; OLIVEIRA, M.; CARDOZO FILHO, J. L.; GALBIERI, R.; GRAMACHO, K. P.; PRATES, M. V.; BLOCH JUNIOR, C. Encrypted antimicrobial peptides from plant proteins. Scientific Reports, v. 7, n. 1, 2017. Article 13263.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | NASCIMENTO, A. dos S.; ALVES, R. M.; SILVA, C. R. de S.; ALBUQUERQUE, P. S. B. de; LEMOS, L. S. L.; GRAMACHO, K. P.; CLÉMENT, D. Transferibilidade e mapeamento de microssatélites entre Theobroma cacao L. e Theobroma grandiflorum (Willd. ex. Spreng.) Schum. Agrotrópica, v. 31, n. 2, p. 85-102, 2019.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | SILVA, S. D. V. M.; LUZ, E. D. M. N.; ALMEIDA, O. C. de; GRAMACHO, K. P. G.; BEZERRA, J. L. Redescrição da sintomatologia causada por Crinipellis perniciosa em cacaueiro. Especial Agrotrópica, Ilhéus, v. 14, n. 1, p. 1-28, abr. 2003. p. 1-28.| Biblioteca(s): Embrapa Rondônia. |
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| 19. | | PEREIRA, J. F.; ALMEIDA, A. P. M. M.; COTA, J.; PAMPHILE, J. A.; SILVA, G. F. da; ARAUJO, E. F. de; GRAMACHO, K. P.; BROMMONSCHENKEL, S. H.; PEREIRA, G. A. G.; QUEIROZ, M. V. de. Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus. Microbiology, Reading, v. 159, p. 112-125, Jan. 2013.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Trigo. |
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| 20. | | LIMA, L. S.; GRAMACHO, K. P.; PIRES, J. L.; CLEMENT, D.; LOPES, U. V.; CARELS, N.; GESTEIRA, A. da S.; GAIOTTO, F. A.; CASCARDO, J. C. de MATTOS; MICHELI, F. Development, characterization, validation, and mapping of SSRs derived from Theobroma cacao L.?Moniliophthora perniciosa interaction ESTs. Tree Genetics & Genomes, Heidelberg, mar. 2010. On line first: 10 march 2010.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 32 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
21/07/2010 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LIMA, L. S.; GRAMACHO, K. P.; PIRES, J. L.; CLEMENT, D.; LOPES, U. V.; CARELS, N.; GESTEIRA, A. da S.; GAIOTTO, F. A.; CASCARDO, J. C. de MATTOS; MICHELI, F. |
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Afiliação: |
Lívia Santos Lima, CEPLAC/CEPEC/SEFIT; Karina Peres Gramacho, CEPLAC/CEPEC/SEFIT; José Luis Pires, CEPLAC/CEPEC/SEFIT; Didier Clement, CIRAD//UMR DAP; Uilson Vanderlei Lopes, CEPLC/CEPEC/SEFIT; Nicolas Carels, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Fernanda Amato Gaiotto, UESC; Júlio Cézar de Mattos Cascardo, UESC; Fabienne Micheli, CIRAD, UMR DAP. |
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Título: |
Development, characterization, validation, and mapping of SSRs derived from Theobroma cacao L.?Moniliophthora perniciosa interaction ESTs. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Tree Genetics & Genomes, Heidelberg, mar. 2010. |
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ISBN: |
1614-2950 (Online) |
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DOI: |
10.1007/s11295-010-0282-1 |
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Idioma: |
Inglês |
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Notas: |
On line first: 10 march 2010. |
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Conteúdo: |
In this study, we report results of the detection and analysis of SSR markers derived of cacao?Moniliophthora perniciosa expressed sequence tags (ESTs) in relation to cacao resistance to witches? broom disease (WBD), and we compare the polymorphism of those ESTs (EST-simple sequence repeat (SSR)) with classical neutral SSR markers. A total of 3,487 ESTs was used in this investigation. SSRs were identified in 430 sequences: 277 from the resistant genotype TSH 1188 and 153 from the susceptible one Catongo, totalizing 505 EST-SSRs with three types of motifs: dinucleotides (72.1%), trinucleotides (27.3%), and tetranucleotides (0.6%). EST-SSRs were classified into 16 main categories; most of the EST-SSRs belonged to ?Unknown function? and ?No homology? categories (45.82%). A high frequency of SSRs was found in the 5?UTR and in the ORF (about 27%) and a low frequency was observed in the 3?UTR (about 8%). Forty-nine ESTSSR primers were designed and evaluated in 21 cacao accessions, 12 revealed polymorphism, having 47 alleles in total, with an average of 3.92 alleles per locus. On the other hand, the 11 genomic SSR markers revealed a total of 47 alleles, with an average of 5.22 alleles per locus. The association of EST-SSR with the genomic SSR enhanced the analysis of genetic distance among the genotypes. Among the 12 polymorphic EST-SSR markers, two were mapped on the F2 Sca 6×ICS 1 population reference for WBD resistance. |
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Palavras-Chave: |
Genetic diversity; Microsatellite; Resistance; Theobroma cacao L; Witches' broom disease. |
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Thesagro: |
Cacau; Doença de Planta; Marcador Molecular; Vassoura de Bruxa. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/858299/1/Development-characterization-validation-and-mapping.pdf
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|
Marc: |
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024 7 $a10.1007/s11295-010-0282-1$2DOI
100 1 $aLIMA, L. S.
245 $aDevelopment, characterization, validation, and mapping of SSRs derived from Theobroma cacao L.?Moniliophthora perniciosa interaction ESTs.$h[electronic resource]
260 $c2010
500 $aOn line first: 10 march 2010.
520 $aIn this study, we report results of the detection and analysis of SSR markers derived of cacao?Moniliophthora perniciosa expressed sequence tags (ESTs) in relation to cacao resistance to witches? broom disease (WBD), and we compare the polymorphism of those ESTs (EST-simple sequence repeat (SSR)) with classical neutral SSR markers. A total of 3,487 ESTs was used in this investigation. SSRs were identified in 430 sequences: 277 from the resistant genotype TSH 1188 and 153 from the susceptible one Catongo, totalizing 505 EST-SSRs with three types of motifs: dinucleotides (72.1%), trinucleotides (27.3%), and tetranucleotides (0.6%). EST-SSRs were classified into 16 main categories; most of the EST-SSRs belonged to ?Unknown function? and ?No homology? categories (45.82%). A high frequency of SSRs was found in the 5?UTR and in the ORF (about 27%) and a low frequency was observed in the 3?UTR (about 8%). Forty-nine ESTSSR primers were designed and evaluated in 21 cacao accessions, 12 revealed polymorphism, having 47 alleles in total, with an average of 3.92 alleles per locus. On the other hand, the 11 genomic SSR markers revealed a total of 47 alleles, with an average of 5.22 alleles per locus. The association of EST-SSR with the genomic SSR enhanced the analysis of genetic distance among the genotypes. Among the 12 polymorphic EST-SSR markers, two were mapped on the F2 Sca 6×ICS 1 population reference for WBD resistance.
650 $aCacau
650 $aDoença de Planta
650 $aMarcador Molecular
650 $aVassoura de Bruxa
653 $aGenetic diversity
653 $aMicrosatellite
653 $aResistance
653 $aTheobroma cacao L
653 $aWitches' broom disease
700 1 $aGRAMACHO, K. P.
700 1 $aPIRES, J. L.
700 1 $aCLEMENT, D.
700 1 $aLOPES, U. V.
700 1 $aCARELS, N.
700 1 $aGESTEIRA, A. da S.
700 1 $aGAIOTTO, F. A.
700 1 $aCASCARDO, J. C. de MATTOS
700 1 $aMICHELI, F.
773 $tTree Genetics & Genomes, Heidelberg, mar. 2010.
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