Data/hora: 10/06/2024 07:36:22

Biblioteca(s): 	Embrapa Mandioca e Fruticultura; Embrapa Meio Ambiente.	
Data corrente: 	07/06/2022	
Data da última atualização: 	08/06/2022	
Autoria: 	AMARAL, M. do; FREITAS, A. C. O.; SANTOS, A. S.; SANTOS, E. C. dos; FERREIRA, M. M.; GESTEIRA, A. da S.; GRAMACHO, K. P.; MARINHO-PRADO, J. S.; PIROVANI, C. P.	
Afiliação: 	MILENA DO AMARAL, UESC; ANA CAMILA OLIVEIRA FREITAS, UESC; ARIANA SILVA SANTOS, UESC; EVERTON CRUZ DOS SANTOS, Instituto do Câncer José Alencar Gomes da Silva; MONALIZA MACÊDO FERREIRA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; KARINA PERES GRAMACHO, CEPEC; JEANNE SCARDINI MARINHO PRADO, CNPMA; CARLOS PRIMINHO PIROVANI, UESC.	
Título: 	TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Scientific Reports, v. 12, n. 1, article 680, 2022.	
DOI: 	https://doi.org/10.1038/s41598-021-04700-y	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Protease inhibitors (PIs) are important biotechnological tools of interest in agriculture. Usually they are the first proteins to be activated in plant-induced resistance against pathogens. Therefore, the aim of this study was to characterize a Theobroma cacao trypsin inhibitor called TcTI. The ORF has 740 bp encoding a protein with 219 amino acids, molecular weight of approximately 23 kDa. rTcTI was expressed in the soluble fraction of Escherichia coli strain Rosetta [DE3]. The purified His-Tag rTcTI showed inhibitory activity against commercial porcine trypsin. The kinetic model demonstrated that rTcTI is a competitive inhibitor, with a Ki value of 4.08 × 10-7 mol L-1. The thermostability analysis of rTcTI showed that 100% inhibitory activity was retained up to 60 °C and that at 70-80 °C, inhibitory activity remained above 50%. Circular dichroism analysis indicated that the protein is rich in loop structures and beta-conformations. Furthermore, in vivo assays against Helicoverpa armigera larvae were also performed with rTcTI in 0.1 mg mL-1 spray solutions on leaf surfaces, which reduced larval growth by 70% compared to the control treatment. Trials with cocoa plants infected with Mp showed a greater accumulation of TcTI in resistant varieties of T. cacao, so this regulation may be associated with different isoforms of TcTI. This inhibitor has biochemical characteristics suitable for biotechnological applications as well as in resistance studies of T. cacao and other crops.	
Thesagro: 	Cacau; Inibidor de Enzima; Praga de Planta; Protease; Resistência; Theobroma Cacao.	
Thesaurus Nal: 	Disease resistance; Helicoverpa armigera; Insecticide resistance; Trypsin inhibitors.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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