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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amapá. Para informações adicionais entre em contato com cpafap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amapá. |
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Data corrente: |
11/09/2014 |
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Data da última atualização: |
06/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; FREITAS-SILVA, O.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G. |
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Afiliação: |
GLAUCIA E. O. MIDORIKAWA, UNB; MARIA DE LOURDES MENDES DE SOUZA, CTAA; OTNIEL FREITAS SILVA, CTAA; JUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP; LUIS I. B. KANZAKI, UNB; ROGERIO E. HANADA, INPA; RENATA M. L. C. MESQUITA, INPA; RIVADALVE COELHO GONCALVES, CPAF-AC; VIRGINIA DE SOUZA ALVARES, CPAF-AC; DANIELA MATIAS DE C BITTENCOURT, CPAA; ROBERTO N. G. MILLER, UNB. |
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Título: |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v. 14, n. 138, 2014. |
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ISSN: |
1471-2180 |
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DOI: |
10.1186/1471-2180-14-138 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. MenosBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius... Mostrar Tudo |
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Palavras-Chave: |
Castanha-do-brasil; Pragas e doenças. |
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Thesagro: |
Aflatoxina. |
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Thesaurus NAL: |
Aspergillus. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02858naa a2200313 a 4500
001 1994761
005 2022-10-06
008 2014 bl uuuu u00u1 u #d
022 $a1471-2180
024 7 $a10.1186/1471-2180-14-138$2DOI
100 1 $aMIDORIKAWA, G. E. O.
245 $aCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.$h[electronic resource]
260 $c2014
520 $aBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species.
650 $aAspergillus
650 $aAflatoxina
653 $aCastanha-do-brasil
653 $aPragas e doenças
700 1 $aSOUZA, M. de L. M. de
700 1 $aFREITAS-SILVA, O.
700 1 $aDIAS, J. do S. A.
700 1 $aKANZAKI, L. I. B.
700 1 $aHANADA, R. E.
700 1 $aMESQUITA, R. M. L. C.
700 1 $aGONCALVES, R. C.
700 1 $aALVARES, V. de S.
700 1 $aBITTENCOURT, D. M. de C.
700 1 $aMILLER, R. N. G.
773 $tBMC Microbiology$gv. 14, n. 138, 2014.
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