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| Registros recuperados : 95 | |
| 81. |  | LIMA, D. de; GONÇALVES, R. C.; CAMPOS, L. J. M.; BARRETO, H. G.; SILVEIRA, M. A. da; ERASMO, E. A. L.; SAGIO, S. A. Physiological and agronomic responses to the application of post-emergence herbicides on sweet potato cultivar selected for its potential as biofuel feedstock. Australian Journal of Crop Science, v. 16, n. 3, p. 349-356, 2022.| Biblioteca(s): Embrapa Soja. |
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| 82. | | TRINDADE, L. de S.; MIDORIKAWA, G. E. O.; MESQUITA, R. M. L. C.; BITTENCOURT, D. de C. M.; GONCALVES, R. C.; MILLER, R. N. G. Molecular identification of member species of Aspergillus section flavi contaminating brazilnut material from Acre and Amazonas. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. 1 CD-ROM. Suplemento. Edição do 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus. Resumo 158.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 83. | | TRINDADE, L. de S.; MINDORIKAWA, G. E. O.; MESQUITA, R. M. L. C.; BITTENCOURT, D. de C. M.; GONCALVES, R. C.; MILLER, R. N. G. Molecular identification of member species of Aspergillus section flavi contaminating brazilnut material from Acre and Amazonas. Tropical Plant Pathology, v. 37, ago 2012. Suplemento. p. 830. Edição dos resumos apresentados no 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus.| Biblioteca(s): Embrapa Acre. |
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| 84. | | FERRAZ, H. G. M.; BADEL, J. L.; GUIMARÃES, L. M. da S.; REIS, B. P.; TÓTOLA, M. R.; GONCALVES, R. C.; ALFENAS, A. C. Xanthomonas axonopodis pv. eucalyptorum pv. nov. causing bacterial leaf blight on eucalypt in Brazil. Plant Pathology, v. 34, n. 4, p. 269-285, 2018.| Biblioteca(s): Embrapa Acre. |
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| 85. | | PANTOJA, K. F. C.; BOARI, A. de J.; MARCHI, B. R. De; REZENDE, J. A. M.; KITAJIMA, E. W.; GONCALVES, R. C.; ASSIS, G. M. L. de; BLAWID, R.; KRAUSE-SAKATE, R. Arachis virus Y, a new potyvirid from Brazilian forage peanut (Arachis pintoi). Archives of Virology, v. 165, n. 10, p. 2349-2353, Oct. 2020.| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Oriental. |
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| 86. | | VALDETARO, D. C. O. F.; HARRINGTON, T. C.; OLIVEIRA, L. S. S.; GUIMARÃES, L. M. S.; MCNEW, D. L.; PIMENTA, L. V. A.; GONCALVES, R. C.; SCHURT, D. A.; ALFENAS, A. C. A host specialized form of Ceratocystis fimbriata causes seed and seedling blight on native Carapa guianensis (andiroba) in Amazonian rainforests. Fungal Biology, v. 123, n. 2, p. 170-182, Feb. 2019.| Biblioteca(s): Embrapa Acre. |
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| 87. | | VALDETARO, D. C. O. F.; HARRINGTON, T. C.; OLIVEIRA, L. S. S.; GUIMARÃES, L. M. S.; MCNEW, D. L.; PIMENTA, L. V. A.; GONCALVES, R. C.; SCHURT, D. A.; ALFENAS, A. C. A host specialized form of Ceratocystis fimbriata causes seed and seedling blight on native Carapa guianensis (andiroba) in Amazonian rainforests. Fungal Biology, v. 123, n. 2, p. 170-182, Feb. 2019.| Biblioteca(s): Embrapa Roraima. |
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| 88. | | RIBEIRO, M. do S.; GONCALVES, R. C.; ALVARES, V. de S.; DIAS, J. do S. A.; ABREU, L. F.; SCHURT, D. A.; PRIMO, H. E. de L.; FREITAS-SILVA, O. Fungos e micotoxinas: estratégias de controle. In: WADT, L. H. de O.; MAROCCOLO, J. F.; GUEDES, M. C.; SILVA, K. E. da (ed.). Castanha-da-amazônia: estudos sobre a espécie e sua cadeia de valor. Brasília, DF: Embrapa, 2023. cap. 1, p. 17-35. V. 2: Pós-coleta e tecnologia de alimentos. ODS 2, ODS 3, ODS 8, ODS 11, ODS 12, ODS 13, ODS 17.| Biblioteca(s): Embrapa Acre; Embrapa Agroindústria de Alimentos; Embrapa Amapá; Embrapa Amazônia Oriental; Embrapa Roraima. |
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| 89. | | GONCALVES, R. C.; ALVARES, V. de S.; CARTAXO, C. B. da C.; WADT, L. H. de O.; SOUZA, J. M. L. de; LIMA, A. C. de; COSTA, D. A. da; GIACOMELLI, M.; MAGALHÃES, K. da S.; MADRUGA, A. L. S. Secador estacionário a ar aquecido forçado artificialmente: inovação tecnológica na secagem de sementes de castanheira-da-amazônia (Bertholletia excelsa). Rio Branco, AC: Embrapa Acre, 2010. 4 p. (Embrapa Acre. Comunicado técnico, 174).| Biblioteca(s): Embrapa Acre. |
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| 90. | | CARTAXO, C. B. da C.; GONCALVES, R. C.; ALVARES, V. de S.; SOUZA, J. M. L. de; GUEDES, M. C.; SCHURT, D. A.; PRIMO, H. E. de L.; FREITAS-SILVA, O.; BOTELHO, S. de C. C.; NOGUEIRA, R. M. Tecnologias de pré-secagem e armazenamento. In: WADT, L. H. de O.; MAROCCOLO, J. F.; GUEDES, M. C.; SILVA, K. E. da (ed.). Castanha-da-amazônia: estudos sobre a espécie e sua cadeia de valor. Brasília, DF: Embrapa, 2023. cap. 2, p. 37-66. V. 2: Pós-coleta e tecnologia de alimentos. ODS 2, ODS 3, ODS 8, ODS 11, ODS 12, ODS 13, ODS 17.| Biblioteca(s): Embrapa Acre; Embrapa Agroindústria de Alimentos; Embrapa Amapá; Embrapa Roraima. |
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| 91. | | AMARAL, E. F. do; BARDALES, N. G.; PEREIRA, J. B. M.; GONÇALVES, R. C.; BERGO, C. L.; ARAÚJO, E. A. de; AMARAL, E. F. de; MELO, A. W. F. de. Zoneamento pedoclimático para a seringueira e o café no município de Capixaba. Rio Branco, AC: Embrapa Acre, 2019. 1 folder.| Biblioteca(s): Embrapa Acre. |
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| 92. | | MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; FREITAS-SILVA, O.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G. Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. BMC Microbiology, v. 14, n. 138, 2014.| Biblioteca(s): Embrapa Amapá. |
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| 93. | | MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G. Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. BMC Microbiology, v.14, n. 138, 2014.| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Ocidental. |
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| 94. | | MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. S.; BITTENCOURT, D. M. C.; MILLER, R. N. G. Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. BMC Microbiology, v. 14, n. 138, 2014.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 95. | | KATO, O. R.; FREITAS, A. C. R. de; FERREIRA, J. H.; LEMOS, W. P.; GONÇALVES, R. C.; RODRIGUES FILHO, J. A.; AZEVEDO, C. M. B. C. de; VASCONCELOS, M. A.; MOURÃO, M.; MATOS, L. M. S. de; GATO, R. F.; SHIMIZU, M.; ROFFE, A. Recuperação de área degradada através do preparo de área sem queima e sistemas agroflorestais. In: CONFERÊNCIA DO SUBPROGRAMA DE CIÊNCIA E TECNOLOGIA - SPC&T FASE II/PPG7, 2008, Belém, PA. Anais... Brasília, DF: CNPq, 2009. p. 494-499. Disponível também em CD-ROM (CD 00173).| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Oriental. |
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| Registros recuperados : 95 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre; Embrapa Amazônia Ocidental. |
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Data corrente: |
02/06/2014 |
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Data da última atualização: |
06/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MIDORIKAWA, G. E. O.; SOUZA, M. de L. M. de; SILVA, O. F.; DIAS, J. do S. A.; KANZAKI, L. I. B.; HANADA, R. E.; MESQUITA, R. M. L. C.; GONCALVES, R. C.; ALVARES, V. de S.; BITTENCOURT, D. M. de C.; MILLER, R. N. G. |
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Afiliação: |
Glaucia E. O. Midorikawa, Universidade de Brasília (UnB); MARIA DE LOURDES MENDES DE SOUZA, CTAA; OTNIEL FREITAS SILVA, CTAA; JUREMA DO SOCORRO AZEVEDO DIAS, CPAF-AP; Luis I. B. Kanzaki, Universidade de Brasília (UnB); Rogerio E. Hanada, Instituto Nacional de Pesquisas da Amazônia (Inpa); Renata M. L. C. Mesquita, Instituto Nacional de Pesquisas da Amazônia (Inpa); RIVADALVE COELHO GONCALVES, CPAF-AC; VIRGINIA DE SOUZA ALVARES, CPAF-Acre; DANIELA MATIAS DE C BITTENCOURT, CPAA; Robert N. G. Miller, Universidade de Brasília (UnB). |
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Título: |
Characterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Microbiology, v.14, n. 138, 2014. |
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ISSN: |
1471-2180 |
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DOI: |
10.1186/1471-2180-14-138 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species. MenosBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius ... Mostrar Tudo |
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Palavras-Chave: |
Castanha do brasil; Castanha-do-brasil; Enfermedades y desórdenes de las plantas; Identificación de patógenos; Nuez del Brasil; Pragas e doenças. |
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Thesagro: |
Aflatoxina; Aspergillus flavus; Bertholletia excelsa; Castanha do pará; Doença de planta; Fungo; Identificação. |
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Thesaurus NAL: |
Amazonia; Aspergillus; Brazil nuts; Fungi; Pathogen identification; Plant diseases and disorders. |
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Categoria do assunto: |
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K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102994/1/castanha-Robert.pdf
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Marc: |
LEADER 03376naa a2200493 a 4500
001 1987473
005 2021-07-06
008 2014 bl uuuu u00u1 u #d
022 $a1471-2180
024 7 $a10.1186/1471-2180-14-138$2DOI
100 1 $aMIDORIKAWA, G. E. O.
245 $aCharacterization of Aspergillus species on Brazil nut from the Brazilian Amazonian region and development of a PCR assay for identification at the genus level.$h[electronic resource]
260 $c2014
520 $aBackground: Brazil nut is a protein-rich extractivist tree crop in the Amazon region. Fungal contamination of shells and kernel material frequently includes the presence of aflatoxigenic Aspergillus species from the section Flavi. Aflatoxins are polyketide secondary metabolites, which are hepatotoxic carcinogens in mammals. The objectives of this study were to identify Aspergillus species occurring on Brazil nut grown in different states in the Brazilian Amazon region and develop a specific PCR method for collective identification of member species of the genus Aspergillus. Results:Polyphasic identification of 137 Aspergillus strains isolated from Brazil nut shell material from cooperatives across the Brazilian Amazon states of Acre, Amapá and Amazonas revealed five species, with Aspergillus section Flavi species A. nomius and A. flavus the most abundant. PCR primers ASP_GEN_MTSSU_F1 and ASP_GEN_MTSSU_R1 were designed for the genus Aspergillus, targeting a portion of the mitochondrial small subunit ribosomal RNA gene. Primer specificity was validated through both electronic PCR against target gene sequences at Genbank and in PCR reactions against DNA from Aspergillus species and other fungal genera common on Brazil nut. Collective differentiation of the observed section Flavi species A. flavus, A. nomius and A. tamarii from other Aspergillus species was possible on the basis of RFLP polymorphism. Conclusions:Given the abundance of Aspergillus section Flavi species A. nomius and A. flavus observed on Brazil nut, and associated risk of mycotoxin accumulation, simple identification methods for such mycotoxigenic species are of importance for Hazard Analysis Critical Control Point system implementation. The assay for the genus Aspergillus represents progress towards specific PCR identification and detection of mycotoxigenic species.
650 $aAmazonia
650 $aAspergillus
650 $aBrazil nuts
650 $aFungi
650 $aPathogen identification
650 $aPlant diseases and disorders
650 $aAflatoxina
650 $aAspergillus flavus
650 $aBertholletia excelsa
650 $aCastanha do pará
650 $aDoença de planta
650 $aFungo
650 $aIdentificação
653 $aCastanha do brasil
653 $aCastanha-do-brasil
653 $aEnfermedades y desórdenes de las plantas
653 $aIdentificación de patógenos
653 $aNuez del Brasil
653 $aPragas e doenças
700 1 $aSOUZA, M. de L. M. de
700 1 $aSILVA, O. F.
700 1 $aDIAS, J. do S. A.
700 1 $aKANZAKI, L. I. B.
700 1 $aHANADA, R. E.
700 1 $aMESQUITA, R. M. L. C.
700 1 $aGONCALVES, R. C.
700 1 $aALVARES, V. de S.
700 1 $aBITTENCOURT, D. M. de C.
700 1 $aMILLER, R. N. G.
773 $tBMC Microbiology$gv.14, n. 138, 2014.
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Embrapa Acre (CPAF-AC) |
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