Data/hora: 10/06/2024 07:57:46

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 03/09/2021
Data da última atualização: | 03/09/2021
Autoria: | PACHECO, T. F.; MACHADO, B. R. C.; MORAIS JÚNIOR, W. G. de; ALMEIDA, J. R. M. de; GONCALVES, S. B.
Afiliação: | THALYTA FRAGA PACHECO, CNPAE; BRENO R. C. MACHADO; WILSON G. DE MORAIS JÚNIOR, Instituto Politécnico do Porto; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; SILVIA BELEM GONCALVES, CNPAE.
Título: | Enhanced tolerance of Spathaspora passalidarum to sugarcane bagasse hydrolysate for ethanol production from xylose.
Complemento do título: | .
Ano de publicação: | 2021
Fonte/Imprenta: | Applied Biochemistry and Biotechnology, v. 193, n. 7, p. 2182-2197, July 2021.
Descrição Física: | PDF: il.
DOI: | https://doi.org/10.1007/s12010-021-03544-6
Idioma: | Inglês
Conteúdo: | Abstract: During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released. These compounds inhibit yeast metabolism, reducing fermentation yields and productivity. This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate. Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors. Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate. At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate. A kinetic model was developed to describe the inhibition of fermentation by substrate and product. The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L. Ethanol concentration that stops cell growth was 30.1 g/L. There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.
Palavras-Chave: | Spathaspora passalidarum.
Thesagro: | Biomassa; Cana de Açúcar; Hidrolise.
Thesaurus Nal: | Biomass; Evolutionary adaptation; Hydrolysates; Lignocellulose; Sugarcane; Xylose.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3932-1) - UPC - AP - DD -  -  -  - DIS