|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
05/09/1994 |
Data da última atualização: |
05/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARAUJO, R. S.; ROBLETO, E. A.; HANDELSMAN, J. |
Afiliação: |
RICARDO SILVA ARAUJO, CNPAF; EDUARDO A. ROBLETO; JO HANDELSMAN. |
Título: |
A hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 60, n. 5, p. 1430-1436, May 1994. |
DOI: |
https://doi.org/10.1128/aem.60.5.1430-1436.1994 |
Idioma: |
Inglês |
Conteúdo: |
We isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003. MenosWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surfa... Mostrar Tudo |
Palavras-Chave: |
Hydrophobic; Mutant; PTCA. |
Thesagro: |
Bactéria; Mutante; Nodulação; Rhizobium. |
Thesaurus Nal: |
nodulation; Rhizobium etli; rhizosphere. |
Categoria do assunto: |
B Sociologia Rural |
Marc: |
LEADER 02428naa a2200277 a 4500 001 1196158 005 2021-10-05 008 1994 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1128/aem.60.5.1430-1436.1994$2DOI 100 1 $aARAUJO, R. S. 245 $aA hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere.$h[electronic resource] 260 $c1994 520 $aWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003. 650 $anodulation 650 $aRhizobium etli 650 $arhizosphere 650 $aBactéria 650 $aMutante 650 $aNodulação 650 $aRhizobium 653 $aHydrophobic 653 $aMutant 653 $aPTCA 700 1 $aROBLETO, E. A. 700 1 $aHANDELSMAN, J. 773 $tApplied and Environmental Microbiology$gv. 60, n. 5, p. 1430-1436, May 1994.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 4 | |
2. | | GONÇALVES, I. G. de; ECHEVARRIA, F. A. M.; FERNANDEZ MUÑOZ, F. Uso do cobre no controle da verminose gastrintestinal em ovinos. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 12.; SEMINÁRIO DE PARASITOSES NA CLÍNICA DE PEQUENOS ANIMAIS, 1.; SEMINÁRIO DE COCCÍDIOS E COCCIDIOSES, 1; SEMINÁRIO DE MANEJO INTEGRADO DA RESISTÊNCIA, 1.; CURSO DE GEOPROCESSAMENTO E SEU USO EM ESTUDOS EPIDEMIOLÓGICOS, 2002, Rio de Janeiro. Anais... Rio de Janeiro: UFRRJ: Colégio Brasileiro de Parasitologia Veterinária, 2002. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sul. |
| |
3. | | BRICARELLO, P. A.; GENNARI, S. M.; OLIVEIRA-SEQUEIRA, T. C. G.; VAZ, C. M. S. L.; GONÇALVES, I. G. de; ECHEVARRIA, F. A. M. Response of Corriedale and Crioula Lanada sheep to artificial primary infection with Haemonchus contortus. Veterinary Research Communications, v. 26, n. 6, p. 447-457, Aug. 2002.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Pecuária Sul. |
| |
4. | | BRICARELLO, P. A.; GENNARI, S. M.; OLIVEIRA-SEQUEIRA, T. C. G.; VAZ, C. M. S. L.; GONÇALVES, I. G. de; ECHEVARRIA, F. A. M. Worm burden and immunological responses in Corriedale and Crioula Lanada sheep following natural infection with Haemonchus contortus. Small Ruminant Research, v. 51, n. 1, p. 75-83, Jan. 2004.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Pecuária Sul. |
| |
Registros recuperados : 4 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|