Data/hora: 08/06/2024 18:27:43

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 11/08/2021
Data da última atualização: | 11/08/2021
Autoria: | GOGONE, I. C. V. P.; FERREIRA, G. H.; GAVA, D.; SCHAEFER, R.; PAULA-LOPES, F. F. de; ROCHA, R. de A.; BARROS, F. R. O. de.
Afiliação: | IZABEL C. V. P. COGONE, UTFPR; GLAUCIA H. FERREIRA, UNIFESP/Diadema; DANIELLE GAVA, CNPSA; REJANE SCHAEFER, CNPSA; FABÍOLA F. DE PAULA-LOPES, UNIFESP/Diadema; RAQUEL DE A. ROCHA, UTFPR; FLAVIA REGINA OLIVEIRA DE BARROS, UTFPR.
Título: | Applicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection.
Ano de publicação: | 2021
Fonte/Imprenta: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 249, n. 119336, 2021.
DOI: | https://doi.org/10.1016/j.saa.2020.119336
Idioma: | Inglês
Conteúdo: | Abstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications.
Palavras-Chave: | Molecular fingerprint; PCV2; PPV; Reproductive failure; Viral diagnostic.
Thesagro: | Diagnostico; Parvovirose; Sanidade Animal; Suíno.
Thesaurus Nal: | Animal diseases; Porcine circovirus-2; Raman spectroscopy; Reproductive success; Swine.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (22187-1) - UPC - AP - DD -  -  -  - DIS