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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
18/10/2010 |
Data da última atualização: |
19/10/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ANDRADE, E. C.; SANTOS, K. C.; MEISSNER, P. E. |
Afiliação: |
EDUARDO CHUMBINHO DE ANDRADE, CNPMF; Keila Cidreira Santos, FAMAM; PAULO ERNESTO MEISSNER FILHO, CNPMF. |
Título: |
Development of degenerated primers to detect different viruses associated with mealybug wilt of pineapple. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Pineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S. |
Idioma: |
Inglês |
Notas: |
Disponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011.
Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010. |
Conteúdo: |
Pineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results show that degenerated primers provided a reliable alternative to plant material certification with about 30-40% on cost reduction and short period of indexing. MenosPineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results s... Mostrar Tudo |
Palavras-Chave: |
Pineapple; Plant disease. |
Thesagro: |
Abacaxi; Doença de Planta. |
Thesaurus Nal: |
Ananas comosus var. comosus. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02561nam a2200205 a 4500 001 1864522 005 2023-10-19 008 2010 bl uuuu u00u1 u #d 100 1 $aANDRADE, E. C. 245 $aDevelopment of degenerated primers to detect different viruses associated with mealybug wilt of pineapple.$h[electronic resource] 260 $aPineapple News, n. 17, p. 8, Aug. 2010. Addendum to Newsletter Pineapple Working Group of the I.S.H.S.$c2010 500 $aDisponível em: < http://www.ishs-horticulture.org/workinggroups/pineapple/PineNews17_addendum.pdf> Acesso em: 16 fev. 2011. Edição de Abstracts of the 7th International Pineapple Symposium, Johor Baru, Malaysia, 2010. 520 $aPineapple (Ananas comosus var. comosus) is propagated vegetatively which facilitates the production of planting material but leads to dissemination of viruses. Mealybug wilt of pineapple (MWP) is a devastating disease of pineapple worldwide. The disease is characterized by severe leaf tip dieback, wilting of the leaves and can lead the plant to die. The MWP is associated with three Ampelovirus species, Pineapple mealybug wilt associated virus-1 (PMWaV-1), PMWaV-2, and PMWaV-3. Besides to the direct damage on plant production, there is great difficulty to select plantlets for planting because some infected plants exhibits no symptoms. The virus can be detected by RT PCR using specific primers; however for phytosanitary purposed, it's interesting to screen plant material for all viruses simultaneously in order to save time and cost. With this objective, degenerate primers were designed and tested to detect PMWaV-1, -2, -3 in pineapple hybrids of Embrapa breeding program. Using the degenerate primers in a RT PCR, it was possible to amplify a fragment of the expected size only in infected samples. Also, the reverse degenerated primer was combined with each of the forward specific primers in order to identify the PMWaV species present in infected plant. These two approaches led to detection of PMWaVs in 20/27 asymptomatic hybrids, with higher prevalence of PMWaV-1 (12 plants), PMWaV-2 (10 plants) and PMWaV-3 (7 plants). A total of 12 plants present mixed infections. The results show that degenerated primers provided a reliable alternative to plant material certification with about 30-40% on cost reduction and short period of indexing. 650 $aAnanas comosus var. comosus 650 $aAbacaxi 650 $aDoença de Planta 653 $aPineapple 653 $aPlant disease 700 1 $aSANTOS, K. C. 700 1 $aMEISSNER, P. E.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
03/12/2018 |
Data da última atualização: |
23/06/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BERTIOLI, S. C. de M. L.; GODOY, I. J.; SANTOS, J. F.; DOYLE, J. J.; GUIMARAES, P. M.; ABERNATHY, B. L.; JACKSON, S. A.; MORETZSOHN, M. de C.; BERTIOLI, D. J. |
Afiliação: |
SORAYA CRISTINA DE M LEAL BERTIOLI, Cenargen; IGNÁCIO J. GODOY, CAMPINAS AGRONOMICAL INSTITUTE; JOÃO F. SANTOS, JOÃO F. SANTOS; JEFF J. DOYLE, CORNELL UNIVERSITY, USA; PATRICIA MESSEMBERG GUIMARAES, Cenargen; BRIAN L. ABERNATHY, UNIVERSITY OF GEORGIA, USA; SCOTT A. JACKSON, UNIVERSITY OF GEORGIA, USA; MARCIO DE CARVALHO MORETZSOHN, Cenargen; DAVID J. BERTIOLI, UNIVERSITY OF GEORGIA, USA. |
Título: |
Segmental allopolyploidy in action: Increasing diversity through polyploid hybridization and homoeologous recombination. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
American Journal of Botany, v. 105, n. 6, p. 1053-1066, 2018. |
DOI: |
10.1002/ajb2.1112 |
Idioma: |
Inglês |
Notas: |
Na publicação: Soraya C. M. Leal-Bertioli; Márcio C. Moretzsohn. |
Palavras-Chave: |
Lineage recombination; Pre-breeding; Segmental allotetraploid; Tetr. |
Thesaurus NAL: |
Arachis; Fabaceae; Introgression; Peanuts; Polyploidy. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/187581/1/Leal-Bertioli-et-al-2018-American-Journal-of-Botany.pdf
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Marc: |
LEADER 01058naa a2200337 a 4500 001 2100586 005 2022-06-23 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1002/ajb2.1112$2DOI 100 1 $aBERTIOLI, S. C. de M. L. 245 $aSegmental allopolyploidy in action$bIncreasing diversity through polyploid hybridization and homoeologous recombination.$h[electronic resource] 260 $c2018 500 $aNa publicação: Soraya C. M. Leal-Bertioli; Márcio C. Moretzsohn. 650 $aArachis 650 $aFabaceae 650 $aIntrogression 650 $aPeanuts 650 $aPolyploidy 653 $aLineage recombination 653 $aPre-breeding 653 $aSegmental allotetraploid 653 $aTetr 700 1 $aGODOY, I. J. 700 1 $aSANTOS, J. F. 700 1 $aDOYLE, J. J. 700 1 $aGUIMARAES, P. M. 700 1 $aABERNATHY, B. L. 700 1 $aJACKSON, S. A. 700 1 $aMORETZSOHN, M. de C. 700 1 $aBERTIOLI, D. J. 773 $tAmerican Journal of Botany$gv. 105, n. 6, p. 1053-1066, 2018.
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