| |
|
|
| Registros recuperados : 554 | |
| 81. |  | PEIXOTO, J. R.; FERRAZ, F. M.; SANTOS, L. C.; ANGELIS, B. de; JULIATTI, F. C. Selecao de genotipos de batata-doce resistentes ao nematoide das galhas (Meloidogyne spp.). Fitopatologia Brasileira, Brasilia, v.23, n.1, p.51-53, mar. 1998.| Biblioteca(s): Embrapa Hortaliças. |
|   |
| 82. | | HAMAWAKI, L. E.; ANDERSEN, O.; HAMAWAKI, O. T.; PEIXOTO, J. R.; MELO, B. de. Control de malas hierbas en el cultivo de la pina cv. Smooth cayenne Curitiba, PR: IAPAR/SBF, 1996 p.430 In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 14. REUNIAO INTERAMERICANA DE HORTICULTURA TROPICAL, 42, 1996, Curitiba, PR.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 83. | | CAMILLO, J.; SCHERWINSKI PEREIRA, J. E.; VIEIRA, R. F.; PEIXOTO, J. R. Conservação in vitro de algodão-do-campo [Cochlospermum regium (Mart. ex scrank) Pilger] - Cochlospermaceae. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p. 318| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 88. | | PEIXOTO, J. R.; MATHIAS FILHO, L.; SILVA, C. M.; OLIVEIRA, C. M.; CECILIO FILHO, A. B. Producao de genotipos de tomateiro tipo salada no periodo de inverno em Araguari. Horticultura Brasileira, Brasilia, v.19, n.2, p.148-150, jul. 2001.| Biblioteca(s): Embrapa Hortaliças. |
|  |
| 89. | | MARTINS, I.; MELLO, S. C. M.; ÁVILA, Z. R.; PÁDUA, R. R.; PEIXOTO, J. R. Potencial de isolados de Trichoderma SPP. para o controle de Colletotrichum gloeosporioides patogênico ao maracujazeiro. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 138.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 91. | | SHIBATA, E. T.; VIEIRA, J. V.; PEIXOTO, J. R.; REIS, A. Prevalência e incidência de patógenos associados com a queima das folhas da cenoura. Horticultura Brasileira, Brasília, DF, v. 25, n. 1, p. s30-s31, ago. 2007. Suplemento. Resumo 156. Trabalho apresentado no 47. Congresso Brasileiro de Olericultura, 4. Simpósio Brasileiro sobre Cucurbitáceas, 2007. Porto Seguro.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 92. | | FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BRAGA, M. F.; PEIXOTO, J. R. Pré-melhoramento do maracujá. In: LOPES, M. A.; FÁVERO, A. P.; FERREIRA, M. A. J. da F.; FALEIRO, F. G.; FOLLE, S. M.; GUIMARÃES, E. P. (Ed.). Pré-melhoramento de plantas: estado da arte e experiências de sucesso. Brasília, DF: Embrapa Informação tecnológica: Embrapa Recursos Genéticos e Biotecnologia; Planaltina, DF: Embrapa Cerrados, 2011. p. 549-570| Biblioteca(s): Embrapa Cerrados. |
| |
| 94. | | JUNQUEIRA, N. T. V.; BRAGA, M. F.; FALEIRO, F. G.; PEIXOTO, J. R.; BERNACCI, L. C. Potencial de espécies silvestres de maracujazeiro como fonte de resistência a doenças. In: FALEIRO, F. G.; JUNQUEIRA, N. T. V.; BRAGA, M. F. (Ed.). Maracujá: germoplasma e melhoramento genético. Planaltina, DF: Embrapa Cerrados, 2005. p. 81-108.| Biblioteca(s): Embrapa Cerrados. |
| |
| 96. | | ABREU, I. M. de O.; JUNQUEIRA, A. M. R.; PEIXOTO, J. R.; OLIVEIRA, S. A. de. Qualidade microbiológica e produtividade de alface sob adubação química e orgânica. Ciência e Tecnologia de Alimentos, Campinas, v. 30, n. 1, Suplemento, p. 108-118, maio 2010.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 99. | | PEIXOTO, J. R.; CAMPOS, A. V. S.; FALEIRO, F. G.; VILELA, M.; PIRES, M. de C. Reação de genótipos de maracujazeiro azedo à bacteriose em condições de campo. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 24., 2016, São Luis. Fruticultura: fruteiras nativas e sustentabilidade. São Luis, MA: SBF, 2016.| Biblioteca(s): Embrapa Cerrados. |
| |
| 100. | | KOSOSKI, R. M.; PEIXOTO, J. R.; JUNQUEIRA, N. T. V.; UESUGI, C. H.; MELO, B. de. Reação de genótipos de maracujazeiro-azedo a Xanthomonas campestris pv. passiflorae, em casa de vegetação. Bioscience Journal, Uberlândia, v. 24, n. 1, p. 60-66, 2008.| Biblioteca(s): Embrapa Cerrados. |
| |
| Registros recuperados : 554 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
|
Data corrente: |
20/04/2016 |
|
Data da última atualização: |
21/07/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 2 |
|
Autoria: |
LACERDA, A. F.; SARTO, R. P. del; SILVA, M. S.; VASCONCELOS, E. A. R. de; COELHO, R. R.; SANTOS, V. O. dos; GODOY, C. V.; SEIXAS, C. D. S.; SILVA, M. C. M. da; GROSSI-DE-SA, M. F. |
|
Afiliação: |
ARIANE FERREIRA LACERDA, Universidade Federal do Rio Grande do Norte; RAFAEL PERSEGHINI DEL SARTO, Centro Universitário do Distrito Federal; MARILIA SANTOS SILVA, Cenargen; ERICO AUGUSTO ROSAS DE VASCONCELOS; ROBERTA RAMOS COELHO; VANESSA OLINTO DOS SANTOS, Universidade Federal do Rio Grande do Norte; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARIA CRISTINA MATTAR DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
|
Título: |
The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
3 Biotech, v. 6, n. 1, p. 59, Feb. 2016. |
|
ISSN: |
2190-5738 |
|
DOI: |
10.1007/s13205-015-0320-7 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Plant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases. MenosPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian ru... Mostrar Tudo |
|
Thesagro: |
Algodão; Doença de planta; Fungo; Soja. |
|
Thesaurus NAL: |
Cotton; Fungi; Plant diseases and disorders; Soybeans. |
|
Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142476/1/the-recombinant-pea....pdf
|
|
Marc: |
LEADER 02860naa a2200349 a 4500
001 2043768
005 2017-07-21
008 2016 bl uuuu u00u1 u #d
022 $a2190-5738
024 7 $a10.1007/s13205-015-0320-7$2DOI
100 1 $aLACERDA, A. F.
245 $aThe recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium, Colletotrichum and Phakopsora.$h[electronic resource]
260 $c2016
520 $aPlant defensins are antifungal peptides produced by the innate immune system plants developed to circumvent fungal infection. The defensin Drr230a, originally isolated from pea, has been previously shown to be active against various entomopathogenic and phytopathogenic fungi. In the present study, the activity of a yeast-expressed recombinant Drr230a protein (rDrr230a) was tested against impacting soybean and cotton fungi. First, the gene was subcloned into the yeast expression vector pPICZαA and expressed in Pichia pastoris. Resulting rDrr230a exhibited in vitro activity against fungal growth and spore germination of Fusarium tucumaniae, which causes soybean sudden death syndrome, and against Colletotrichum gossypii var. cephalosporioides, which causes cotton ramulosis. The rDrr230a IC50 corresponding to inhibition of fungal growth of F. tucumaniae and C. gossypii var. cephalosporioides was 7.67 and 0.84 µM, respectively, demonstrating moderate activity against F. tucumaniae and high potency against C. gossypii var. cephalosporioides. Additionally, rDrr230a at 25 ng/µl (3.83 µM) resulted in 100 % inhibition of spore germination of both fungi, demonstrating that rDrr230a affects fungal development since spore germination. Moreover, rDrr230a at 3 µg/µl (460.12 µM) inhibited 100 % of in vitro spore germination of the obligatory biotrophic fungus Phakopsora pachyrhizi, which causes Asian soybean rust. Interestingly, rDrr230a substantially decreased the severity of Asian rust, as demonstrated by in planta assay. To our knowledge, this is the first report of a plant defensin active against an obligatory biotrophic phytopathogenic fungus. Results revealed the potential of rDrr230a as a candidate to be used in plant genetic engineering to control relevant cotton and soybean fungal diseases.
650 $aCotton
650 $aFungi
650 $aPlant diseases and disorders
650 $aSoybeans
650 $aAlgodão
650 $aDoença de planta
650 $aFungo
650 $aSoja
700 1 $aSARTO, R. P. del
700 1 $aSILVA, M. S.
700 1 $aVASCONCELOS, E. A. R. de
700 1 $aCOELHO, R. R.
700 1 $aSANTOS, V. O. dos
700 1 $aGODOY, C. V.
700 1 $aSEIXAS, C. D. S.
700 1 $aSILVA, M. C. M. da
700 1 $aGROSSI-DE-SA, M. F.
773 $t3 Biotech$gv. 6, n. 1, p. 59, Feb. 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
