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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
13/12/1999 |
Data da última atualização: |
13/12/1999 |
Autoria: |
AVILA, A. C.; BEEK, M. A.; DUSI, A. N. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Purificacao e sorologia para o virus de vira-cabeca do tomateiro (TSMV). |
Ano de publicação: |
1988 |
Fonte/Imprenta: |
Fitopatologia Brasileira, Brasilia, v.13, n.2, p.127, jul. 1988. |
Idioma: |
Português |
Notas: |
Resumo. |
Palavras-Chave: |
Purification; Sorologia; TSVW; Vira-cabeca. |
Thesagro: |
Purificação; Vírus. |
Thesaurus Nal: |
Nicotiana rustica; serology. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00638naa a2200241 a 4500 001 1766332 005 1999-12-13 008 1988 bl uuuu u00u1 u #d 100 1 $aAVILA, A. C. 245 $aPurificacao e sorologia para o virus de vira-cabeca do tomateiro (TSMV). 260 $c1988 500 $aResumo. 650 $aNicotiana rustica 650 $aserology 650 $aPurificação 650 $aVírus 653 $aPurification 653 $aSorologia 653 $aTSVW 653 $aVira-cabeca 700 1 $aBEEK, M. A. 700 1 $aDUSI, A. N. 773 $tFitopatologia Brasileira, Brasilia$gv.13, n.2, p.127, jul. 1988.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
27/10/2010 |
Data da última atualização: |
04/06/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
GIARETTA, D. R.; BOGO, A.; COELHO, C. M. M.; GUIDOLIN, A. F.; DANTAS, A. C. de M.; GOMES, E. A. |
Afiliação: |
Diorvania Ribeiro Giaretta, UDESC; Amauri Bogo, UDESC; Cleide Maria Medeiros Coelho, UDESC; Altamir Frederico Guidolin, UDESC; Adriana Cibele de Mesquita Dantas, UFSC; ELIANE APARECIDA GOMES, CNPMS. |
Título: |
ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple glomerella leaf spot. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Ciência Rural, Santa Maria, v. 40, n. 4, p. 806-812, abr. 2010. |
DOI: |
10.1590/S0103-84782010005000051 |
Idioma: |
Inglês |
Conteúdo: |
Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp were obtained. The sequencing of this region allowed the generation of a phylogenetic tree, regardless of their hosts, and 5 isolated groups were obtained. From the "in silico" comparison, it was possible to verify a variation from 93 to 100% of similarity between the sequences studied and the Genbank data base. The causal agent of GLS is nearly related (clustered) to isolates of pineapple guava and to the citrus isolates used as control. |
Palavras-Chave: |
Árvore filogenética; Filogenia molecular; Goiabeira serrana. |
Thesaurus NAL: |
Citrus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30974/1/its-rdna.pdf
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Marc: |
LEADER 01947naa a2200241 a 4500 001 1865333 005 2018-06-04 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1590/S0103-84782010005000051$2DOI 100 1 $aGIARETTA, D. R. 245 $aITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple glomerella leaf spot.$h[electronic resource] 260 $c2010 520 $aSeveral diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp were obtained. The sequencing of this region allowed the generation of a phylogenetic tree, regardless of their hosts, and 5 isolated groups were obtained. From the "in silico" comparison, it was possible to verify a variation from 93 to 100% of similarity between the sequences studied and the Genbank data base. The causal agent of GLS is nearly related (clustered) to isolates of pineapple guava and to the citrus isolates used as control. 650 $aCitrus 653 $aÁrvore filogenética 653 $aFilogenia molecular 653 $aGoiabeira serrana 700 1 $aBOGO, A. 700 1 $aCOELHO, C. M. M. 700 1 $aGUIDOLIN, A. F. 700 1 $aDANTAS, A. C. de M. 700 1 $aGOMES, E. A. 773 $tCiência Rural, Santa Maria$gv. 40, n. 4, p. 806-812, abr. 2010.
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Embrapa Milho e Sorgo (CNPMS) |
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