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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/11/2017 |
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Data da última atualização: |
24/11/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
DENARDIN, I. C.; LAZZAROTTO, M.; LAZZAROTTO, S. R. da S.; MAGALHAES, W. L. E.; QUEIROZ, D. L. de. |
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Afiliação: |
Isabella Cristina Denardin, UFPR; MARCELO LAZZAROTTO, CNPF; Simone Rosa da Silveira Lazzarotto, Pós-graduanda da UEPG; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; DALVA LUIZ DE QUEIROZ, CNPF. |
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Título: |
Caracterização térmica de óleos essenciais de eucalipto visando material genético resistente ao ataque de Glycaspis brimblecombei. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE ANÁLISE TÉRMICA, 8., 2017, Ponta Grossa. Livro de resumos. [Ponta Grossa: UEPG, 2017]. |
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Páginas: |
p. 125-127. |
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Idioma: |
Português |
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Conteúdo: |
O ataque de Glycaspis brimblecombei a plantações de eucaliptos podem provocar grandes perdas de produção. Estudo anterior apresenta relação entre as características térmicas de óleos essenciais de folhas de eucaliptos com a preferência do ataque desta praga. Com o objetivo de identificar potenciais materiais genéticos resistentes e susceptíveis ao ataque do psilídeo foram extraídos por hidrodestilação óleos essenciais de folhas de 5 espécies de eucalipto. Foram realizadas análises termogravimétricas destes óleos essenciais. Observou-se que os óleos essenciais apresentaram características térmicas diferentes. Avaliando os resultados pode-se selecionar óleo essencial de Eucalyptus benthamii como material genético potencialmente resistente ao ataque de Glycaspis brimblecombei. Para a espécie E. dunni são necessários de mais estudos. Os resultados das análises térmicas tem grande potencial em auxiliar o melhoramento genético de materiais vegetais. |
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Palavras-Chave: |
Análise térmica; Psilídeo; Resistência a pragas. |
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Thesagro: |
Eucalipto; Melhoramento Genético Vegetal; Óleo essencial. |
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Thesaurus Nal: |
Eucalyptus; Thermal analysis. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167454/1/2017-MarceloL-SAT-CarcterizacaoGlycaspis.pdf
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Marc: |
LEADER 01887nam a2200265 a 4500
001 2080688
005 2017-11-24
008 2017 bl uuuu u00u1 u #d
100 1 $aDENARDIN, I. C.
245 $aCaracterização térmica de óleos essenciais de eucalipto visando material genético resistente ao ataque de Glycaspis brimblecombei.$h[electronic resource]
260 $aIn: SIMPÓSIO DE ANÁLISE TÉRMICA, 8., 2017, Ponta Grossa. Livro de resumos. [Ponta Grossa: UEPG$c2017
300 $ap. 125-127.
520 $aO ataque de Glycaspis brimblecombei a plantações de eucaliptos podem provocar grandes perdas de produção. Estudo anterior apresenta relação entre as características térmicas de óleos essenciais de folhas de eucaliptos com a preferência do ataque desta praga. Com o objetivo de identificar potenciais materiais genéticos resistentes e susceptíveis ao ataque do psilídeo foram extraídos por hidrodestilação óleos essenciais de folhas de 5 espécies de eucalipto. Foram realizadas análises termogravimétricas destes óleos essenciais. Observou-se que os óleos essenciais apresentaram características térmicas diferentes. Avaliando os resultados pode-se selecionar óleo essencial de Eucalyptus benthamii como material genético potencialmente resistente ao ataque de Glycaspis brimblecombei. Para a espécie E. dunni são necessários de mais estudos. Os resultados das análises térmicas tem grande potencial em auxiliar o melhoramento genético de materiais vegetais.
650 $aEucalyptus
650 $aThermal analysis
650 $aEucalipto
650 $aMelhoramento Genético Vegetal
650 $aÓleo essencial
653 $aAnálise térmica
653 $aPsilídeo
653 $aResistência a pragas
700 1 $aLAZZAROTTO, M.
700 1 $aLAZZAROTTO, S. R. da S.
700 1 $aMAGALHAES, W. L. E.
700 1 $aQUEIROZ, D. L. de
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Gado de Leite. |
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Data corrente: |
06/12/2011 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GOZZO, V. C.; OKURA, V. K.; FONSECA, I.; MARTINS, M. F.; CARDOSO, F. F.; GIACHETTO, P. F. |
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Afiliação: |
VINÍCIUS CAMPIDELLI GOZZO, Unicamp/CNPTIA; VAGNER KATSUMI OKURA, Unicamp; ISABELA FONSECA, UFV; MARTA FONSECA MARTINS, CNPGL; FERNANDO FLORES CARDOSO, CPPSUL; POLIANA FERNANDA GIACHETTO, CNPTIA. |
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Título: |
Identification of mechanisms involved in mastitis response by means of gene network building. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional, 2011. |
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Páginas: |
Não paginado. |
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Idioma: |
Inglês |
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Notas: |
X-MEETING 2011. |
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Conteúdo: |
Mastitis, an inflammation of the mammary gland, is the most prevalent and costly production disease in dairy herds worldwide. It is caused generally by bacteria and accounts for a significant decrease in milk production and quality. One promising approach to reduce problems caused by mastitis, in addition to sanitary care, is the selection of animals resistant to disease and the incorporation of this trait into the herds. Therefore, studies to better understand the mechanisms involved in animal response to this disease are essential to the proposition of new advances in area. In this context, the aim of this study was to identify groups of genes involved in cow response to mastitis infection, through gene network building from microarray data. Gene expression data from the GeneChipâ Bovine Genome Array (Affymetrix) hybridization with milk somatic cells samples from Holstein-Zebu crossbreed dairy cows, obtained before (B) and 24 hrs after (A) artificial infection with Staphylococcus agalactiae, were analyzed using a network building methodology based on gene co-expression. We used WGCNA (Weighted Gene Co-expression Analysis), a systems biology method for describing the correlation patterns among genes across microarray samples, that can be used for finding clusters (modules) of highly correlated genes to identify modules of co-expressed genes, which may correspond to functionally related genes. By comparing two networks (between contrasting data sets), conserved and non-conserved modules can be identified. This strategy, named differential network analysis, aims to identify genes groups that are both differentially expressed and differentially connected, and changes in connectivity may correspond to large-scale rewiring, in response to environmental changes and/or physiologic perturbations. Two microarray data sets, B (n=5) and A (n=5), were preprocessed using affy and gcrma R/Bioconductor packages. A filter was applied, which resulted in the use of only those transcripts present in all samples. Gene co-expression networks were identified separately for each group (B and A), by the R/WGCNA package. Gene networks were compared between the two groups, and non-conserved modules were uncovered from a correlation test of the connectivity values. Our analysis identified a total of 17 modules of co-expressed genes, three of them, designed by the colors grey (n=35), blue (n=37) and turquoise (n=192), non-conserved between the groups. Using Blast2GO for enrichment analysis, we find the molecular function Protein Binding overrepresented in all three modules. However, in despite of the same molecular function, each one of the modules showed distinct characteristcs. Genes of grey module (BTG3, CD3E, MBD1, CHIC2, PLXNA3, MOCS3, NEIL1, VPS45, BCL2) were related to apoptosis and antigen recognition. Genes of turquoise module were enriched in inflammation mediators, including known mastitis marker genes (FGL1, GJA1, F2RL1, PTPRF, S100A2, TGFB2). The blue one uncovered genes involved in cell division and inflammatory response (CD97, MAD2L1, ZFP106, CDKN2C, LOC514364, NOP14, PCBD1, LOC100139798, AP1S1, EDN1, IL1B, ANXA11). Our study identified some mechanisms (represented by gene modules) that have changed in cows in early response to mastitis infection. Further analysis are being carried out, based on these results, to advance the understanding of animals response to the disease, which can lead to identify the candidate genes that could be used in breeding programs. MenosMastitis, an inflammation of the mammary gland, is the most prevalent and costly production disease in dairy herds worldwide. It is caused generally by bacteria and accounts for a significant decrease in milk production and quality. One promising approach to reduce problems caused by mastitis, in addition to sanitary care, is the selection of animals resistant to disease and the incorporation of this trait into the herds. Therefore, studies to better understand the mechanisms involved in animal response to this disease are essential to the proposition of new advances in area. In this context, the aim of this study was to identify groups of genes involved in cow response to mastitis infection, through gene network building from microarray data. Gene expression data from the GeneChipâ Bovine Genome Array (Affymetrix) hybridization with milk somatic cells samples from Holstein-Zebu crossbreed dairy cows, obtained before (B) and 24 hrs after (A) artificial infection with Staphylococcus agalactiae, were analyzed using a network building methodology based on gene co-expression. We used WGCNA (Weighted Gene Co-expression Analysis), a systems biology method for describing the correlation patterns among genes across microarray samples, that can be used for finding clusters (modules) of highly correlated genes to identify modules of co-expressed genes, which may correspond to functionally related genes. By comparing two networks (between contrasting data sets), conserved and non-conse... Mostrar Tudo |
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Palavras-Chave: |
Evolution and phylogeny; Expressão gênica; Mastite. |
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Thesaurus NAL: |
Gene expression; genomics; Mastitis; sequence analysis. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/49479/1/mastitis.pdf
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Marc: |
LEADER 04550nam a2200277 a 4500
001 1908744
005 2020-01-24
008 2011 bl uuuu u00u1 u #d
100 1 $aGOZZO, V. C.
245 $aIdentification of mechanisms involved in mastitis response by means of gene network building.
260 $aIn: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 7.; INTERNATIONAL CONFERENCE OF THE IBEROAMERICAN SOCIETY FOR BIOINFORMATICS, 3., 2011, Florianópolis. Proceedings... Florianópolis: Associação Brasileira de Bioinformática e Biologia Computacional$c2011
300 $aNão paginado.
500 $aX-MEETING 2011.
520 $aMastitis, an inflammation of the mammary gland, is the most prevalent and costly production disease in dairy herds worldwide. It is caused generally by bacteria and accounts for a significant decrease in milk production and quality. One promising approach to reduce problems caused by mastitis, in addition to sanitary care, is the selection of animals resistant to disease and the incorporation of this trait into the herds. Therefore, studies to better understand the mechanisms involved in animal response to this disease are essential to the proposition of new advances in area. In this context, the aim of this study was to identify groups of genes involved in cow response to mastitis infection, through gene network building from microarray data. Gene expression data from the GeneChipâ Bovine Genome Array (Affymetrix) hybridization with milk somatic cells samples from Holstein-Zebu crossbreed dairy cows, obtained before (B) and 24 hrs after (A) artificial infection with Staphylococcus agalactiae, were analyzed using a network building methodology based on gene co-expression. We used WGCNA (Weighted Gene Co-expression Analysis), a systems biology method for describing the correlation patterns among genes across microarray samples, that can be used for finding clusters (modules) of highly correlated genes to identify modules of co-expressed genes, which may correspond to functionally related genes. By comparing two networks (between contrasting data sets), conserved and non-conserved modules can be identified. This strategy, named differential network analysis, aims to identify genes groups that are both differentially expressed and differentially connected, and changes in connectivity may correspond to large-scale rewiring, in response to environmental changes and/or physiologic perturbations. Two microarray data sets, B (n=5) and A (n=5), were preprocessed using affy and gcrma R/Bioconductor packages. A filter was applied, which resulted in the use of only those transcripts present in all samples. Gene co-expression networks were identified separately for each group (B and A), by the R/WGCNA package. Gene networks were compared between the two groups, and non-conserved modules were uncovered from a correlation test of the connectivity values. Our analysis identified a total of 17 modules of co-expressed genes, three of them, designed by the colors grey (n=35), blue (n=37) and turquoise (n=192), non-conserved between the groups. Using Blast2GO for enrichment analysis, we find the molecular function Protein Binding overrepresented in all three modules. However, in despite of the same molecular function, each one of the modules showed distinct characteristcs. Genes of grey module (BTG3, CD3E, MBD1, CHIC2, PLXNA3, MOCS3, NEIL1, VPS45, BCL2) were related to apoptosis and antigen recognition. Genes of turquoise module were enriched in inflammation mediators, including known mastitis marker genes (FGL1, GJA1, F2RL1, PTPRF, S100A2, TGFB2). The blue one uncovered genes involved in cell division and inflammatory response (CD97, MAD2L1, ZFP106, CDKN2C, LOC514364, NOP14, PCBD1, LOC100139798, AP1S1, EDN1, IL1B, ANXA11). Our study identified some mechanisms (represented by gene modules) that have changed in cows in early response to mastitis infection. Further analysis are being carried out, based on these results, to advance the understanding of animals response to the disease, which can lead to identify the candidate genes that could be used in breeding programs.
650 $aGene expression
650 $agenomics
650 $aMastitis
650 $asequence analysis
653 $aEvolution and phylogeny
653 $aExpressão gênica
653 $aMastite
700 1 $aOKURA, V. K.
700 1 $aFONSECA, I.
700 1 $aMARTINS, M. F.
700 1 $aCARDOSO, F. F.
700 1 $aGIACHETTO, P. F.
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Embrapa Agricultura Digital (CNPTIA) |
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