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| Registros recuperados : 54 | |
| 4. |  | KAEL, D. C.; GERHARDT, I. R. Estabelecimento de uma coleção de plasmídeos para transformação genética de eucalipto. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 11., 2012, Colombo. Anais. Colombo: Embrapa Florestas, 2012. (Embrapa Florestas. Documentos, 240). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Biblioteca(s): Embrapa Florestas. |
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| 9. | | ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 10. | | ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 322. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Biblioteca(s): Embrapa Florestas. |
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| 12. | | GRUNENNVALDT, R. L.; KAEL, D. C.; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M.; GERHARDT, I. R. Identificação e clonagem de promotores raiz-específicos de eucalipto. Biochemistry and Biotechnology Reports, v. 2, n. 3, p. 50-53, 2013. Edição dos anais do 3º Simpósio de Bioquímica e Biotecnologia, 2013. Londrina.| Biblioteca(s): Embrapa Florestas. |
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| 13. | | GERHARDT, I. R.; OKURA, V. K.; DANTE, R. A.; ARRUDA, P. A gene expression atlas of Vellozia nivea, a desiccation-tolerant species from the Brazilian campos rupestres. In: INTERNATIONAL PLANT & ANIMAL GENOME, 28., 2020, San Diego. Abstracts... Livingston, NJ: Scherago International, 2020. PE1028.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 17. | | LAIA, M. L. de; GERHARDT, I. R.; ABAD, J. I. M.; DEGENHARDT-GOLDBACH, J.; GONÇALVES, J. F.; MISSIAGGIA, A. A. A biotecnologia e o eucalipto do futuro. In: VALE, A. B. do; MACHADO, C. C.; PIRES, J. M. M.; VILAR, M. B.; COSTA, C. B.; NACIF, A. de P. (Ed.). Eucaliptocultura no Brasil: silvicultura, manejo e ambiência. Viçosa, MG: SIF, 2014. p. 39-67.| Biblioteca(s): Embrapa Florestas. |
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| 18. | | GERHARDT, I. R.; GIACHETTO, P. F.; YAMAGISHI, M. E. B.; LOBO, F. P.; TEIXEIRA, J.; PENCHEL, R. M.; MISSIAGGIA, A. Comparative transcriptome analysis of eucalyptus genotypes that differ in carbon allocation. In: INTERNATIONAL PLANT & ANIMAL GENOME, 20., 2012, San Diego. Abstract... Jersey City: Scherago International, 2012. Não paginado. 1 pôster.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Florestas. |
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| 19. | | GERHARDT, I. R.; LEITE, A.; PAES, N.; ROMANO, E.; AZEVEDO, M.; BLOCH JUNIOR, C.; GROSSI de SA, M. F. Isolation and partial characterization of a genomic clone of arcelin-5 protein. In: ENCUENTRO LATINO AMERICANO DE BIOTECNOLOGIA VEGETAL, 2., 1995, Puerto Iguazu, Argentina. REDBIO95. [S.l.: s.n.], 1995. n.D-11.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | FERREIRA, P.; PAES, N. S.; SOUZA, C. R. B.; GERHARDT, I. R.; VENTURA, M.; SA, M. F. G. de. Arcelin protein: antigenic relations, developing seeds study and imunocytolocalization. In: ENCONTRO BRASILEIRO DE BIOTECNOLOGIA VEGETAL, 1., 1993, Brasilia. Programa e resumos. Brasilia: EMBRAPA-CENARGEN, 1993. Poster 031. Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. |
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Afiliação: |
LAUDIANE ZANELLA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JOÃO CARLOS BESPALHOK FILHO, UFPR. |
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Título: |
RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
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Conteúdo: |
From the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants. MenosFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histoch... Mostrar Tudo |
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Thesaurus NAL: |
Populus alba; Populus tremula. |
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Categoria do assunto: |
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Marc: |
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100 1 $aZANELLA, L.
245 $aRNA interference supression of DOF transcription factor from Populus tremula x Populus alba.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 322$c2019
500 $aNa publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants.
650 $aPopulus alba
650 $aPopulus tremula
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGERHARDT, I. R.
700 1 $aBESPALHOK FILHO, J. C.
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