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| 5. |  | GASPARI-PEZZOPANE, C. de; FAVARIN, J. L.; MALUF, M. P.; PEZZOPANE, J. R. M.; MISTRO, J. C.; SALGADO, P. R. Metodologia para avaliação do comportamento fenológico de cultivares de Coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia. Anais... Brasília, DF: Embrapa Café, 2007.| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 5 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café; Embrapa Unidades Centrais. |
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Data corrente: |
28/06/2013 |
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Data da última atualização: |
28/04/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
GASPARI-PEZZOPANE, C. de; BONTURI, N.; GUERREIRO FILHO, O.; FAVARIN, J. L.; MALUF, M. P. |
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Afiliação: |
MIRIAN PEREZ MALUF, SAPC. |
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Título: |
Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 47, n. 7, p. 972-982, jul. 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to identify genes that could be used as suitable markers for molecular recognition of phenological stages during coffee (Coffea arabica) fruit development. Four cultivars were evaluated as to their differential Expression of genes associated to fruit development and maturation processes. GEne expression was characterized by both semi?quantitative and quantitative RT?PCR, in fruit harvested at seven different developmental stages, during three different seasons. No size polymorphisms or differential expression were observed among the cultivars for the evaluated genes; however, distinct expression profiles along fruit development were determined for each gene. Four out of the 28 evaluated genes exhibited a regular expression profile in all cultivars and harvest seasons, and, therefore, they were validated as candidate phenological markers of coffee fruit. The gene ??galactosidase can be used as a marker of green stage, caffeine synthase as a marker of transition to green and yellowish?green stages, and isocitrate lyase and ethylene receptor 3 as markers of late maturation. |
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Palavras-Chave: |
Cup quality; Differential expression; Fruit phenology; QRT?PCR; Qualidade de bebida. |
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Thesagro: |
Coffea Arábica; Maturação. |
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Thesaurus NAL: |
Ripening. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142639/1/Gene-expression-profile-during-coffee.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/85020/1/Gene-expression-profile-during.pdf
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Marc: |
LEADER 01912naa a2200265 a 4500
001 2044180
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008 2012 bl uuuu u00u1 u #d
100 1 $aGASPARI-PEZZOPANE, C. de
245 $aGene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
260 $c2012
520 $aThe objective of this work was to identify genes that could be used as suitable markers for molecular recognition of phenological stages during coffee (Coffea arabica) fruit development. Four cultivars were evaluated as to their differential Expression of genes associated to fruit development and maturation processes. GEne expression was characterized by both semi?quantitative and quantitative RT?PCR, in fruit harvested at seven different developmental stages, during three different seasons. No size polymorphisms or differential expression were observed among the cultivars for the evaluated genes; however, distinct expression profiles along fruit development were determined for each gene. Four out of the 28 evaluated genes exhibited a regular expression profile in all cultivars and harvest seasons, and, therefore, they were validated as candidate phenological markers of coffee fruit. The gene ??galactosidase can be used as a marker of green stage, caffeine synthase as a marker of transition to green and yellowish?green stages, and isocitrate lyase and ethylene receptor 3 as markers of late maturation.
650 $aRipening
650 $aCoffea Arábica
650 $aMaturação
653 $aCup quality
653 $aDifferential expression
653 $aFruit phenology
653 $aQRT?PCR
653 $aQualidade de bebida
700 1 $aBONTURI, N.
700 1 $aGUERREIRO FILHO, O.
700 1 $aFAVARIN, J. L.
700 1 $aMALUF, M. P.
773 $tPesquisa Agropecuária Brasileira$gv. 47, n. 7, p. 972-982, jul. 2012.
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Registro original: |
Embrapa Café (CNPCa) |
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