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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
09/12/2019 |
Data da última atualização: |
09/12/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SOARES, M. P.; SHIROMA, L. S.; CARDOSO, I. L.; SAMPAIO, F. G.; JONSSON, C. M.; RANTIN, F. T.; QUEIROZ, S. C. do N. de. |
Afiliação: |
M. P. SOARES, UFSCar; L. S. SHIROMA, Unicamp; I. L. CARDOSO, UFSCar; FERNANDA GARCIA SAMPAIO, CNPMA; CLAUDIO MARTIN JONSSON, CNPMA; F. T. RANTIN, UFSCar; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA. |
Título: |
A rapid ultrasound-assisted dispersive liquid-liquid microextraction followed by liquid chromatography coupled to a mass spectrometry for determination of artemisinin in tilapia muscle. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 48., 2019, Milan. [Abstracts...] Milan: University of Milano-Bicocca, 2019. Ref. P 136. |
Idioma: |
Português |
Conteúdo: |
Intensive aquaculture represents stressful conditions for fish and can trigger diseases that negatively affect their performance and survival. Fish production requires greater control, with improved health conditions and food quality, concerned with consuming products free of therapeutic products and synthetic chemicals. The use of active ingredients extracted from plants is an alternative to improve the immune system of these animals, reducing problems with stress and the emergence of diseases. Artemisinin (ART), isolated from the Artemisia annua plant, is a promising and potent antimalarial drug that meets the challenge of resistant parasites. ART, an endoperoxide containing sesquiterpene lactone, is the main component responsible for the therapeutic effect. In aquaculture, herbal medicines have recently appeared in the framework of complementary and alternative medicines, because they have relatively lower cost and safety. However, careful may be taken with the application method considering environmental issues, since the administration directly in water causes an accelerated decomposition of compounds, restricting the additional use of the herbal medicine. Thinking of oral administration, many of these compounds are not completely metabolized by the animal?s organism, being eliminated in urine and feces. These residues can accumulate in the soil, undergo leaching, transported to the water sources and contaminate the aquatic compartment. In addition, these compounds may accumulate in medicated fish tissues for the purpose of preventing or treating infections, leading to problems in the consumption of these foods. Therefore, to evaluate the accumulation of residues in fish, an analytical method based on high performance liquid chromatography coupled to ion trap mass spectrometry (LC-MS/MS) was developed to quantify ART in tilapia muscles (Oreochromis niloticus). For sample preparation, 1 g of fish and 5 mL of disperser extractor acetonitrile were placed in tubes and vortexed for 1 minute. Then, 2 g of magnesium sulfate and 50 mg of C18 were added for lipid removal and again stirred in the vortex for 1 minute. The tubes were centrifuged at 3000 x g for 5 minutes. For ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME), 2 mL of the supernatant were transferred to centrifuge tubes, 2 mL of Milli-Q water was added and 250 ?L of toluene, for three times, was added, with a micro syringe. The tubes were left on ultrasound for 1 minute and centrifuged at 3,000 × g for 5 minutes. The supernatant was removed and dried with nitrogen, resuspended in 250 ?L of mobile phase, filtered with 0.22 ?m nylon filters and injected for LC-MS/MS analysis. The mobile phase, composed of ammonium acetate 10 mmol L-1:acetonitrile, 50:50 (v/v) was used. The proposed method was validated and successfully applied in pharmacokinetics studies. MenosIntensive aquaculture represents stressful conditions for fish and can trigger diseases that negatively affect their performance and survival. Fish production requires greater control, with improved health conditions and food quality, concerned with consuming products free of therapeutic products and synthetic chemicals. The use of active ingredients extracted from plants is an alternative to improve the immune system of these animals, reducing problems with stress and the emergence of diseases. Artemisinin (ART), isolated from the Artemisia annua plant, is a promising and potent antimalarial drug that meets the challenge of resistant parasites. ART, an endoperoxide containing sesquiterpene lactone, is the main component responsible for the therapeutic effect. In aquaculture, herbal medicines have recently appeared in the framework of complementary and alternative medicines, because they have relatively lower cost and safety. However, careful may be taken with the application method considering environmental issues, since the administration directly in water causes an accelerated decomposition of compounds, restricting the additional use of the herbal medicine. Thinking of oral administration, many of these compounds are not completely metabolized by the animal?s organism, being eliminated in urine and feces. These residues can accumulate in the soil, undergo leaching, transported to the water sources and contaminate the aquatic compartment. In addition, these compounds may ... Mostrar Tudo |
Thesagro: |
Tilápia. |
Thesaurus Nal: |
Artemisinin; Liquid chromatography. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206510/1/RA-SampaioFG-et-al-HPLC2019-P136.pdf
|
Marc: |
LEADER 03772nam a2200217 a 4500 001 2116384 005 2019-12-09 008 2019 bl uuuu u00u1 u #d 100 1 $aSOARES, M. P. 245 $aA rapid ultrasound-assisted dispersive liquid-liquid microextraction followed by liquid chromatography coupled to a mass spectrometry for determination of artemisinin in tilapia muscle.$h[electronic resource] 260 $aIn: INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 48., 2019, Milan. [Abstracts...] Milan: University of Milano-Bicocca, 2019. Ref. P 136.$c2019 520 $aIntensive aquaculture represents stressful conditions for fish and can trigger diseases that negatively affect their performance and survival. Fish production requires greater control, with improved health conditions and food quality, concerned with consuming products free of therapeutic products and synthetic chemicals. The use of active ingredients extracted from plants is an alternative to improve the immune system of these animals, reducing problems with stress and the emergence of diseases. Artemisinin (ART), isolated from the Artemisia annua plant, is a promising and potent antimalarial drug that meets the challenge of resistant parasites. ART, an endoperoxide containing sesquiterpene lactone, is the main component responsible for the therapeutic effect. In aquaculture, herbal medicines have recently appeared in the framework of complementary and alternative medicines, because they have relatively lower cost and safety. However, careful may be taken with the application method considering environmental issues, since the administration directly in water causes an accelerated decomposition of compounds, restricting the additional use of the herbal medicine. Thinking of oral administration, many of these compounds are not completely metabolized by the animal?s organism, being eliminated in urine and feces. These residues can accumulate in the soil, undergo leaching, transported to the water sources and contaminate the aquatic compartment. In addition, these compounds may accumulate in medicated fish tissues for the purpose of preventing or treating infections, leading to problems in the consumption of these foods. Therefore, to evaluate the accumulation of residues in fish, an analytical method based on high performance liquid chromatography coupled to ion trap mass spectrometry (LC-MS/MS) was developed to quantify ART in tilapia muscles (Oreochromis niloticus). For sample preparation, 1 g of fish and 5 mL of disperser extractor acetonitrile were placed in tubes and vortexed for 1 minute. Then, 2 g of magnesium sulfate and 50 mg of C18 were added for lipid removal and again stirred in the vortex for 1 minute. The tubes were centrifuged at 3000 x g for 5 minutes. For ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME), 2 mL of the supernatant were transferred to centrifuge tubes, 2 mL of Milli-Q water was added and 250 ?L of toluene, for three times, was added, with a micro syringe. The tubes were left on ultrasound for 1 minute and centrifuged at 3,000 × g for 5 minutes. The supernatant was removed and dried with nitrogen, resuspended in 250 ?L of mobile phase, filtered with 0.22 ?m nylon filters and injected for LC-MS/MS analysis. The mobile phase, composed of ammonium acetate 10 mmol L-1:acetonitrile, 50:50 (v/v) was used. The proposed method was validated and successfully applied in pharmacokinetics studies. 650 $aArtemisinin 650 $aLiquid chromatography 650 $aTilápia 700 1 $aSHIROMA, L. S. 700 1 $aCARDOSO, I. L. 700 1 $aSAMPAIO, F. G. 700 1 $aJONSSON, C. M. 700 1 $aRANTIN, F. T. 700 1 $aQUEIROZ, S. C. do N. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registros recuperados : 71 | |
5. | | ROSA, P.; RAGAGNIN, M.; GARCIA, J. M. J.; OLIVEIRA, C. S. Cytoplasmic granules in oocytes do not influence embryonic and early fetal development in bovine. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 33., 2019, Ilha da Comandatuba, BA. Proceedings... Ilha da Comandatuba, BA: SBTE, 2019. p. 609.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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6. | | GARCIA, J. M.; LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S. Evaluation of L-carnitine supplementation on the production and vitrification of bovine embryos produced in vitro. Animal Reproduction, v. 14, n. 3, p. 882, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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7. | | LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. The use of linoleic acid in in vitro culture of bovine embryos and its effects on production and survival to vitrification. Animal Reproduction, v. 14, n. 3, p. 894, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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9. | | MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. Zygote, v. 15, p. 295-306, nov. 2007.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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10. | | OLIVEIRA, C. S.; JEDRUSIK, A.; SARAIVA, N. Z.; CAMARGO, L. S. de A.; GARCIA, J. M.; ZERNICKA-GOETZ, M. PAR6C polarity protein expression pattern in male and female bovine embryos. Animal Reproduction, v. 11, n. 3, p. 455, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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11. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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12. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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13. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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19. | | SARAIVA, N. Z.; FIGUEIRO, M. R.; MARQUES, J. R. F.; LIMA, M. R. de; GARCIA, J. M. Effect of in vitro co-culture of buffalo embryos with bovine cumulus cells on the potential for early embryonic development. Animal Reproduction, v. 14, n. 3, p. 858, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Amazônia Oriental. |
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20. | | ROSA, P. M. S.; MONTEIRO, C. A. S.; LEAL, G. R.; CAMARGO, A. J. R.; SERAPIÃO, R. V.; LIMA, M. R.; GARCIA, J. M.; OLIVEIRA, C. S. Association of two epigenetic modulators on the initial development of female bovine embryos. Animal Reproduction, v. 15, n. 3, p. 484, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 71 | |
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