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| 8. |  | GARCIA, J. M. L.; LEITE, D. L.; KUHN, G. (org.). Zelo no suo de instrumentos de trabalho "cuide bem dos equipamentos". Pelotas: Embrapa Clima Temperado, 2006. 26 p. (Embrapa Clima Temperado. Documentos, 159). Autores: Alberto Carlos Signorini, Carlos Eloi Braga Ribeiro, Carmem Lourdes Pauletto Chemello, Claiton Amaral Kuhn, Claudinei Bonemann Rosso, Claudio Ruas Schimulfening, Daniela Lopes Leite, Denise Durte dos Santos, Fernando de Souza...| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | GARCIA, J. M.; LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S. Evaluation of L-carnitine supplementation on the production and vitrification of bovine embryos produced in vitro. Animal Reproduction, v. 14, n. 3, p. 882, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | DAYAN, A.; FIRMINO, F. A.; AVELINO, K. B.; VANTINI, R.; GARCIA, J. M. Aspiração folicular em novilhas nelore pré púberes e produção "in vitro" de embriões (resultados preliminares). Ciência Animal, v. 7, n. 2, p. 107, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | ROSA, P.; RAGAGNIN, M.; GARCIA, J. M. J.; OLIVEIRA, C. S. Cytoplasmic granules in oocytes do not influence embryonic and early fetal development in bovine. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 33., 2019, Ilha da Comandatuba, BA. Proceedings... Ilha da Comandatuba, BA: SBTE, 2019. p. 609.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | GARCIA, J. M.; RODRIGUES, C. F. M.; DAYAN, A.; AVELINO, K. B.; VANTINI, R. Produção in vitro de embriões bovinos em vacas com problemas reprodutivos adquiridos (Resultados Preliminares). Ciência Animal, v. 7, n. 2, p. 108, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. The use of linoleic acid in in vitro culture of bovine embryos and its effects on production and survival to vitrification. Animal Reproduction, v. 14, n. 3, p. 894, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/06/2011 |
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Data da última atualização: |
27/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
MINGOTI, G. Z.; CASTRO, V. S. D. C.; NICIURA, S. C. M.; BARRETO, L. S. S.; GARCIA, J. M. |
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Afiliação: |
GISELE ZOCCAL MINGOTI, UNESP/ARAÇATUBA; VIVIANE SGGOBI DIAS CAIADO CASTRO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; LETÍCIA SIQUEIRA SÁ BARETTO, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In Vitro Cell Dev Biol Anim., v. 47, n. 5-6, p.361-367, jun. 2011. |
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DOI: |
10.1007/s11626-011-9400-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Aiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2. MenosAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addit... Mostrar Tudo |
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Palavras-Chave: |
Embryo; In vitro maturation; Macromolecule. |
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Thesaurus NAL: |
beef cattle; oxygen. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
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024 7 $a10.1007/s11626-011-9400-0$2DOI
100 1 $aMINGOTI, G. Z.
245 $aThe effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development.$h[electronic resource]
260 $c2011
520 $aAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2.
650 $abeef cattle
650 $aoxygen
653 $aEmbryo
653 $aIn vitro maturation
653 $aMacromolecule
700 1 $aCASTRO, V. S. D. C.
700 1 $aNICIURA, S. C. M.
700 1 $aBARRETO, L. S. S.
700 1 $aGARCIA, J. M.
773 $tIn Vitro Cell Dev Biol Anim.$gv. 47, n. 5-6, p.361-367, jun. 2011.
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