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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
21/01/2010 |
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Data da última atualização: |
04/02/2010 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
GARCIA, S. A. L. |
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Afiliação: |
Suzana Antunes Lourenço Garcia, UFLA. |
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Título: |
Identificação, validação e uso de marcadores moleculares a partir de sequências de DNA dos genomas de Mycosphaerella fijiensis e de Musa spp. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
2009. |
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Páginas: |
74 f. |
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Idioma: |
Inglês
Português |
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Notas: |
Dissertação (Mestre em Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras. Manoel Teixeira Souza Júnior, Orientador. Cláudia Fortes Ferreira, CNPMF, Co-orientador. |
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Conteúdo: |
Mycosphaerella fijiensis is the causal agent of black leaf streak of banana, the most important threat to banana production in many countries and particularly in Costa Rica where the climate is very conducive for the disease. Currently, the main control measure is the frequent application of fungicides. However, apart from environmental concerns, this approach is not sustainable due to the abrupt or gradual development of fungicide resistance. To analyze the population dynamics of fungicide resistance, we developed a molecular diagnostics for strobilurin resistance, using the cytochrome b gene (cytb), in M. fijiensis. We also developed molecular markers for the mating type idiomorphs (mat1-1 and mat1-2) and primers for five VNTR loci to estimate population genetic parameters. Monospore isolates were collected at three plantations that are 20-30 km apart (Cartagena, San Pablo and Zent) in the Limón province that represents the heart of the Costa Rican banana production area. Ninety-five isolates were obtained from a distant wild-type population that was never sprayed with fungicides in the Herédia province. In total, 665 isolates were assayed for mat1-1, mat1-2, VNTR and cytb. The mating type genes segregated in a 1:1 ratio indicating that the sampled populations most likely are randomly mating. The VNTR primers identified 32 alleles, among them, 21 were identified in wild type population, 20 in San Pablo population, 23 in Zent population and 24 in Cartegena population. The strobilurin diagnostic indicated that the wildtype population is entirely sensitive and that two of the three commercial populations, San Pablo and Cartagena are entirely resistant. The Zent population contained 8% of sensitive strains even though strobilurins were still used in that plantation. The next steps will be analysis of gene diversity within each population and the levels of gene flow. These analyses will provide an excellent basis for future research into fungicide resistance in Costa Rica populations of M. fijiensis as well as comparative analyses with other banana-producing areas. MenosMycosphaerella fijiensis is the causal agent of black leaf streak of banana, the most important threat to banana production in many countries and particularly in Costa Rica where the climate is very conducive for the disease. Currently, the main control measure is the frequent application of fungicides. However, apart from environmental concerns, this approach is not sustainable due to the abrupt or gradual development of fungicide resistance. To analyze the population dynamics of fungicide resistance, we developed a molecular diagnostics for strobilurin resistance, using the cytochrome b gene (cytb), in M. fijiensis. We also developed molecular markers for the mating type idiomorphs (mat1-1 and mat1-2) and primers for five VNTR loci to estimate population genetic parameters. Monospore isolates were collected at three plantations that are 20-30 km apart (Cartagena, San Pablo and Zent) in the Limón province that represents the heart of the Costa Rican banana production area. Ninety-five isolates were obtained from a distant wild-type population that was never sprayed with fungicides in the Herédia province. In total, 665 isolates were assayed for mat1-1, mat1-2, VNTR and cytb. The mating type genes segregated in a 1:1 ratio indicating that the sampled populations most likely are randomly mating. The VNTR primers identified 32 alleles, among them, 21 were identified in wild type population, 20 in San Pablo population, 23 in Zent population and 24 in Cartegena population. The s... Mostrar Tudo |
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Palavras-Chave: |
Fungos; Transcriptoma; Trap; VNTR. |
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Thesagro: |
Banana; DNA; Genoma; Marcador Molecular; Sigatoka Negra. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02909nam a2200241 a 4500
001 1656314
005 2010-02-04
008 2009 bl uuuu m 00u1 u #d
100 1 $aGARCIA, S. A. L.
245 $aIdentificação, validação e uso de marcadores moleculares a partir de sequências de DNA dos genomas de Mycosphaerella fijiensis e de Musa spp.
260 $a2009.$c2009
300 $a74 f.
500 $aDissertação (Mestre em Biotecnologia Vegetal) - Universidade Federal de Lavras, Lavras. Manoel Teixeira Souza Júnior, Orientador. Cláudia Fortes Ferreira, CNPMF, Co-orientador.
520 $aMycosphaerella fijiensis is the causal agent of black leaf streak of banana, the most important threat to banana production in many countries and particularly in Costa Rica where the climate is very conducive for the disease. Currently, the main control measure is the frequent application of fungicides. However, apart from environmental concerns, this approach is not sustainable due to the abrupt or gradual development of fungicide resistance. To analyze the population dynamics of fungicide resistance, we developed a molecular diagnostics for strobilurin resistance, using the cytochrome b gene (cytb), in M. fijiensis. We also developed molecular markers for the mating type idiomorphs (mat1-1 and mat1-2) and primers for five VNTR loci to estimate population genetic parameters. Monospore isolates were collected at three plantations that are 20-30 km apart (Cartagena, San Pablo and Zent) in the Limón province that represents the heart of the Costa Rican banana production area. Ninety-five isolates were obtained from a distant wild-type population that was never sprayed with fungicides in the Herédia province. In total, 665 isolates were assayed for mat1-1, mat1-2, VNTR and cytb. The mating type genes segregated in a 1:1 ratio indicating that the sampled populations most likely are randomly mating. The VNTR primers identified 32 alleles, among them, 21 were identified in wild type population, 20 in San Pablo population, 23 in Zent population and 24 in Cartegena population. The strobilurin diagnostic indicated that the wildtype population is entirely sensitive and that two of the three commercial populations, San Pablo and Cartagena are entirely resistant. The Zent population contained 8% of sensitive strains even though strobilurins were still used in that plantation. The next steps will be analysis of gene diversity within each population and the levels of gene flow. These analyses will provide an excellent basis for future research into fungicide resistance in Costa Rica populations of M. fijiensis as well as comparative analyses with other banana-producing areas.
650 $aBanana
650 $aDNA
650 $aGenoma
650 $aMarcador Molecular
650 $aSigatoka Negra
653 $aFungos
653 $aTranscriptoma
653 $aTrap
653 $aVNTR
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