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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/04/2004 |
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Data da última atualização: |
06/04/2004 |
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Autoria: |
JORGE, V; FREGENE, M; BONIARBALE, M; TOHME, J; VERDIER, V. |
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Título: |
Molecular genetic mapping of resistance to xanthomosas axonopodis pv. manihotis in cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.41, nov., 1998., Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Bacterial Blight (CBB), caused by xanthomonm axonopodis pv. manihotis (Xwn) is one of the most important diseases of cassava in Africa and South America. Deployment of resistant varieties is one of the major approach to controll the disease. CBB resistance has been devoloped from M. esculenta and the wild relative M. glaziovii. Genetic mapping in an intraspecific Fl cross (TMS 30572 x CM 2177-2) has led to the first molecular genetic map of cassava. Segregation of molecular markers has been analyzed in 90 individuals of the Fl population. In order to understand the genetic determinism of resistance to CBB, the cassava map has been completed analyzing the segregation of 53 RFLP and RAPD markers in the reimander 60 El individuals. We observed that the linkage groups are well conserved. Resistance of the150 Fl individuals has been assessed under controlled conditions both by ,t semi-quantitative evaluation (Symptom scale: 0 to 5) and a quantitative evaluation of the vascular contamination. Different Xam strains were used (Clo-84, Clo-1. Clo-136, Clo-295 and ORST X-27).Statistical analysis on individual phenotype were conducted with the computer package Q-gene regarding each component of resistance as a potential quantitative trait (QTL). Regions of the cassava genome have been identified controlling from 10-22'1/0 of the phenotypic variance for resistance to different Xam straings. Different linkage groups are involved in resistance to difference Xam strains. The QTLs are associated with the C, D, N and X linkage groups. A backcross population, derived from crossing members of the mapping population has been developed and will provide opportunities for recombination between markers and resistance genes. MenosAbstract: Bacterial Blight (CBB), caused by xanthomonm axonopodis pv. manihotis (Xwn) is one of the most important diseases of cassava in Africa and South America. Deployment of resistant varieties is one of the major approach to controll the disease. CBB resistance has been devoloped from M. esculenta and the wild relative M. glaziovii. Genetic mapping in an intraspecific Fl cross (TMS 30572 x CM 2177-2) has led to the first molecular genetic map of cassava. Segregation of molecular markers has been analyzed in 90 individuals of the Fl population. In order to understand the genetic determinism of resistance to CBB, the cassava map has been completed analyzing the segregation of 53 RFLP and RAPD markers in the reimander 60 El individuals. We observed that the linkage groups are well conserved. Resistance of the150 Fl individuals has been assessed under controlled conditions both by ,t semi-quantitative evaluation (Symptom scale: 0 to 5) and a quantitative evaluation of the vascular contamination. Different Xam strains were used (Clo-84, Clo-1. Clo-136, Clo-295 and ORST X-27).Statistical analysis on individual phenotype were conducted with the computer package Q-gene regarding each component of resistance as a potential quantitative trait (QTL). Regions of the cassava genome have been identified controlling from 10-22'1/0 of the phenotypic variance for resistance to different Xam straings. Different linkage groups are involved in resistance to difference Xam strains. The QT... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02246naa a2200169 a 4500
001 1651840
005 2004-04-06
008 1998 bl uuuu u00u1 u #d
100 1 $aJORGE, V
245 $aMolecular genetic mapping of resistance to xanthomosas axonopodis pv. manihotis in cassava.
260 $c1998
520 $aAbstract: Bacterial Blight (CBB), caused by xanthomonm axonopodis pv. manihotis (Xwn) is one of the most important diseases of cassava in Africa and South America. Deployment of resistant varieties is one of the major approach to controll the disease. CBB resistance has been devoloped from M. esculenta and the wild relative M. glaziovii. Genetic mapping in an intraspecific Fl cross (TMS 30572 x CM 2177-2) has led to the first molecular genetic map of cassava. Segregation of molecular markers has been analyzed in 90 individuals of the Fl population. In order to understand the genetic determinism of resistance to CBB, the cassava map has been completed analyzing the segregation of 53 RFLP and RAPD markers in the reimander 60 El individuals. We observed that the linkage groups are well conserved. Resistance of the150 Fl individuals has been assessed under controlled conditions both by ,t semi-quantitative evaluation (Symptom scale: 0 to 5) and a quantitative evaluation of the vascular contamination. Different Xam strains were used (Clo-84, Clo-1. Clo-136, Clo-295 and ORST X-27).Statistical analysis on individual phenotype were conducted with the computer package Q-gene regarding each component of resistance as a potential quantitative trait (QTL). Regions of the cassava genome have been identified controlling from 10-22'1/0 of the phenotypic variance for resistance to different Xam straings. Different linkage groups are involved in resistance to difference Xam strains. The QTLs are associated with the C, D, N and X linkage groups. A backcross population, derived from crossing members of the mapping population has been developed and will provide opportunities for recombination between markers and resistance genes.
700 1 $aFREGENE, M
700 1 $aBONIARBALE, M
700 1 $aTOHME, J
700 1 $aVERDIER, V.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.41, nov., 1998., Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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