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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
16/06/2000 |
Data da última atualização: |
16/06/2000 |
Autoria: |
BRAZ, R. F.; GALVAO, J. C. C.; FINGER, F. L.; MIRANDA, G. V.; PUIATTI, M. |
Título: |
Efeito da temperatura e do acondicionamento na perda de peso pos-colheita de espigas de milho-verde. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 23., 2000, Uberlandia, MG. A inovacao tecnologica e a competitividade no contexto dos mercados globalizados: [anais]... Sete Lagoas: ABMS / Embrapa Milho e Sorgo / Universidade Federal de Uberlandia, 2000. |
Idioma: |
Português |
Notas: |
CD-ROM. |
Palavras-Chave: |
Hibrido AG 1051; Hidrido DINA 170; Milho-verde; Postharvest; Sweet corn; Weight losses. |
Thesagro: |
Acondicionamento; Armazenamento; Perda de Peso; Pós-Colheita; Temperatura; Zea Mays. |
Thesaurus Nal: |
hybrids; packaging; storage; temperature. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01153naa a2200361 a 4500 001 1767404 005 2000-06-16 008 2000 bl uuuu u00u1 u #d 100 1 $aBRAZ, R. F. 245 $aEfeito da temperatura e do acondicionamento na perda de peso pos-colheita de espigas de milho-verde. 260 $c2000 500 $aCD-ROM. 650 $ahybrids 650 $apackaging 650 $astorage 650 $atemperature 650 $aAcondicionamento 650 $aArmazenamento 650 $aPerda de Peso 650 $aPós-Colheita 650 $aTemperatura 650 $aZea Mays 653 $aHibrido AG 1051 653 $aHidrido DINA 170 653 $aMilho-verde 653 $aPostharvest 653 $aSweet corn 653 $aWeight losses 700 1 $aGALVAO, J. C. C. 700 1 $aFINGER, F. L. 700 1 $aMIRANDA, G. V. 700 1 $aPUIATTI, M. 773 $tIn: CONGRESSO NACIONAL DE MILHO E SORGO, 23., 2000, Uberlandia, MG. A inovacao tecnologica e a competitividade no contexto dos mercados globalizados: [anais]... Sete Lagoas: ABMS / Embrapa Milho e Sorgo / Universidade Federal de Uberlandia, 2000.
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
15/03/2019 |
Data da última atualização: |
18/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FERNANDES, M. S.; GARCIA, L. C.; NASCIMENTO, R. C.; SANTANA, H.; MORAIS, P. B.; CAPDEVILLE, G. de; BRASIL, B. dos S. A. F. |
Afiliação: |
Maiara S. Fernandes; LORENA COSTA GARCIA CALSING, CNPAE; Rodrigo C. Nascimento; Hugo Santana; Priscila B. Morais; GUY DE CAPDEVILLE, CNPAE; BRUNO DOS SANTOS A F BRASIL, SPD. |
Título: |
Customized cryopreservation protocols for chlorophytes based on cell morphology. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Algal Research, v. 38, 2019. |
Idioma: |
Português |
Conteúdo: |
The development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains present higher survival rates when cryopreserved at late-log phase. Such information is of valuable practical use since a simple visual inspection of algae strain's predominant morphotype can guide the choice of the most suitable cryopreservation protocol. MenosThe development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains prese... Mostrar Tudo |
Palavras-Chave: |
Central composite rotational design; Morphotype. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02383naa a2200217 a 4500 001 2107118 005 2019-11-18 008 2019 bl uuuu u00u1 u #d 100 1 $aFERNANDES, M. S. 245 $aCustomized cryopreservation protocols for chlorophytes based on cell morphology.$h[electronic resource] 260 $c2019 520 $aThe development of efficient algae cryopreservation methods is pivotal to the establishment of long-term culture collections as well as algae breeding and genetic modification programs. However, the unpredictable responses of distinct algal species to cryopreservation agents and protocols have hampered the standardization of universal methods so far. The results presented in this study indicate that intrinsic biological factors (i.e.: cell morphology and phylogenetic origin) play a role in the definition of which culture age and cryoprotectant agent type and concentration should be used to achieve successful cryopreservation. Through the use of Central Composite Rotatable Design (CCRD) it was possible to define optimized protocols for cryopreserving three major morphotypic groups found among chlorophytes (i.e.: coenobium, coccoid or palmella forming strains). These optimized protocols were then validated upon fifteen strains from Sphaeropleales, Chlamydomonadales and Chlorelalles clades. Analysis reveals that the use of DMSO as the sole cryoprotectant provided the highest postfreezing cell viability recovery rates for 80% of the coccoid strains tested, while the combination of glycerol and PEG400 allowed efficient cryopreservation of 100% of the coenobium and palmella forming strains evaluated. In addition, results suggest that coenobium-forming strains have higher resistance to freeze/thawing when frozen at mid-log phase of growth, while coccoid and plamelloid strains present higher survival rates when cryopreserved at late-log phase. Such information is of valuable practical use since a simple visual inspection of algae strain's predominant morphotype can guide the choice of the most suitable cryopreservation protocol. 653 $aCentral composite rotational design 653 $aMorphotype 700 1 $aGARCIA, L. C. 700 1 $aNASCIMENTO, R. C. 700 1 $aSANTANA, H. 700 1 $aMORAIS, P. B. 700 1 $aCAPDEVILLE, G. de 700 1 $aBRASIL, B. dos S. A. F. 773 $tAlgal Research$gv. 38, 2019.
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