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| Registros recuperados : 81 | |
| 8. |  | GARCIA, J. M. L.; LEITE, D. L.; KUHN, G. (org.). Zelo no suo de instrumentos de trabalho "cuide bem dos equipamentos". Pelotas: Embrapa Clima Temperado, 2006. 26 p. (Embrapa Clima Temperado. Documentos, 159). Autores: Alberto Carlos Signorini, Carlos Eloi Braga Ribeiro, Carmem Lourdes Pauletto Chemello, Claiton Amaral Kuhn, Claudinei Bonemann Rosso, Claudio Ruas Schimulfening, Daniela Lopes Leite, Denise Durte dos Santos, Fernando de Souza...| Biblioteca(s): Embrapa Unidades Centrais. |
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| 10. | | DAYAN, A.; FIRMINO, F. A.; AVELINO, K. B.; VANTINI, R.; GARCIA, J. M. Aspiração folicular em novilhas nelore pré púberes e produção "in vitro" de embriões (resultados preliminares). Ciência Animal, v. 7, n. 2, p. 107, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. The use of linoleic acid in in vitro culture of bovine embryos and its effects on production and survival to vitrification. Animal Reproduction, v. 14, n. 3, p. 894, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | ROSA, P.; RAGAGNIN, M.; GARCIA, J. M. J.; OLIVEIRA, C. S. Cytoplasmic granules in oocytes do not influence embryonic and early fetal development in bovine. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY, 33., 2019, Ilha da Comandatuba, BA. Proceedings... Ilha da Comandatuba, BA: SBTE, 2019. p. 609.| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | GARCIA, J. M.; LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S. Evaluation of L-carnitine supplementation on the production and vitrification of bovine embryos produced in vitro. Animal Reproduction, v. 14, n. 3, p. 882, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | GARCIA, J. M.; RODRIGUES, C. F. M.; DAYAN, A.; AVELINO, K. B.; VANTINI, R. Produção in vitro de embriões bovinos em vacas com problemas reprodutivos adquiridos (Resultados Preliminares). Ciência Animal, v. 7, n. 2, p. 108, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; LIMA, M. R. DE; GARCIA, J. M. Global H3K9AC and H3K27ME3 expression in blastomeres from 8-to 16-cell stage bovine embryos. Reproduction, Fertility and Development , v. 26, n. 1, p. 148-149, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 81 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
14/02/2011 |
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Data da última atualização: |
19/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CHIARATTI, M. R.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; SANGALLI, J. R.; MESQUITA, L. G.; BALIEIRO, J. C. de C.; SMITH, L. C.; GARCIA, J. M.; MEIRELLES, F. V. |
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Afiliação: |
MARCOS ROBERTO CHIARATTI, USP/PIRASSUNGA; CHRISTINA RAMIRES FERREIRA, USP/PIRASSUNGA; FELIPE PERECIN, USP/PIRASSUNUNGA; SIMONE CRISTINA MEO NICIURA, CPPSE; JULIANO RODRIGUES SANGALLI, USP/PIRASSUNUGA; LÍGIA GARCIA MESQUITA, USP/PIRASSUNGA; JÚLIO CÉSAR DE CARVALHO BALIEIRO, USP/PIRASSUNUNGA; LAWRENCE CHARLES SMITH, UNIVERSITE DE MONTREAL/SAINT-HYACINTHE; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL; FLÁVIO VIEIRA MEIRELLES, USP/PIRASSUNUNGA. |
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Título: |
Ooplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Reproductive BioMedicine Online v. 22, n. 22, p. 172-183, 2011. |
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DOI: |
https://doi.org/10.1016/j.rbmo.2010.10.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Ooplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes. MenosOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to ... Mostrar Tudo |
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Palavras-Chave: |
Embryo; Ethidium bromide; Oocyte; Ooplasm; Transfer. |
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Thesaurus NAL: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/876985/1/OoplastMediatedDevelopmental.pdf
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Marc: |
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100 1 $aCHIARATTI, M. R.
245 $aOoplast-mediated developmental rescue of bovine oocytes exposed to ethidium bromide.$h[electronic resource]
260 $c2011
520 $aOoplasm transfer has been used successfully to treat infertility in women with ooplasmic insufficiency and has culminated in the birth of healthy babies. To investigate whether mitochondrial dysfunction is a factor in ooplasmic insufficiency, bovine oocytes were exposed to ethidium bromide, an inhibitor of mitochondrial DNA replication and transcription, during in-vitro maturation (IVM). Exposure of immature oocytes to ethidium bromide for 24?h during IVM hampered meiotic resumption and the migration of cortical granules. However, a briefer treatment with ethidium bromide during the last 4?h of IVM led to partial arrest of preimplantation development without affecting oocyte maturation. Ooplasm transfer was then performed to rescue the oocytes with impaired development. In spite of this developmental hindrance, transfer of normal ooplasm into ethidium bromide-treated oocytes resulted in a complete rescue of embryonic development and the birth of heteroplasmic calves. Although this study unable to determine whether developmental rescue occurred exclusively through introduction of unaffected mitochondria into ethidium bromide-damaged oocytes, e.g. ethidium bromide may also affect other ooplasm components, these results clearly demonstrate that ooplasm transfer can completely rescue developmentally compromised oocytes, supporting the potential use of ooplasm transfer in therapeutic applications. Although the transfer of egg cytoplasm between women has been used successfully to rescue development in infertile patients culminating in the birth of healthy babies, the aetiology of egg-related infertility remains poorly understood. To verify the role played by a dysfunction in the mitochondrial activity, bovine eggs were exposed to ethidium bromide, a drug commonly used to impair mitochondrial function. We show that eggs exposed to ethidium bromide arrest embryonic development after IVF. Moreover, a complete rescue of development was observed after transfer of cytoplasm from untreated eggs into ethidium bromide-treated eggs leading to the birth of heteroplasmic calves which contained mitochondria from donor and recipient eggs (heteroplasmy). Although we were unable to confirm that mitochondria were the only ooplasm component affected by ethidium bromide, we provide evidence that cytoplasm transfer can completely rescue developmentally compromised oocytes.
650 $amitochondrial DNA
653 $aEmbryo
653 $aEthidium bromide
653 $aOocyte
653 $aOoplasm
653 $aTransfer
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aNICIURA, S. C. M.
700 1 $aSANGALLI, J. R.
700 1 $aMESQUITA, L. G.
700 1 $aBALIEIRO, J. C. de C.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
700 1 $aMEIRELLES, F. V.
773 $tReproductive BioMedicine Online$gv. 22, n. 22, p. 172-183, 2011.
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