| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Cerrados. |
|
Data corrente: |
17/12/2014 |
|
Data da última atualização: |
12/02/2015 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A. |
|
Afiliação: |
FURGS; FURGS; RODRIGO DA ROCHA FRAGOSO, CPAC; WAGNER ALEXANDRE LUCENA, CNPA; IAPAR; UNIVERSITY OF CALDAS, COLOMBIA; INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, FRANCE; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN; ERIKA VALERIA SALIBA ALBUQUERQUE FR, CENARGEN. |
|
Título: |
Molecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Gene, v. 553, n. 1, p. 7-16, Dec. 2014. |
|
DOI: |
10.1016/j.gene.2014.09.050 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies. MenosAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal... Mostrar Tudo |
|
Palavras-Chave: |
Expressão gênica. |
|
Thesagro: |
Amido; Curculionideo; Hidrolise; Praga de planta. |
|
Thesaurus Nal: |
Curculionidae; Enzymatic hydrolysis; Gene expression; Insect pests; Starch. |
|
Categoria do assunto: |
O Insetos e Entomologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114033/1/34279.pdf
|
|
Marc: |
LEADER 02768naa a2200385 a 4500
001 2003023
005 2015-02-12
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.gene.2014.09.050$2DOI
100 1 $aBEZERRA, C. A.
245 $aMolecular cloning and characterization of an a-amylase cDNA highly expressed in major feeding stages of the coffee berry borer, Hypothenemus hampei.
260 $c2014
520 $aAbstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect a-amylases, including Anthonomus grandis, Ips typographus and Sitophilus oryzae a-amylases. In depth analysis revealed that the highly conserved three amino acid residues (Asp184, Glu220, and Asp285), which compose the catalytic site are also presented in AmyHha amylase. The AmyHha gene seems to be a single copy in the haploid genome and AmyHha transcription levels were found higher in L2 larvae and adult insects, both corresponding to major feeding phases. Modeling of the AmyHha predicted protein uncovered striking structural similarities to the Tenebrio molitor a-amylase also displaying the same amino acid residues involved in enzyme catalysis (Asp184, Glu220 and Asp285). Since AmyHha gene was mostly transcribed in the intestinal tract of H. hampei larvae, the cognate a-amylase could be considered a high valuable target to coffee bean insect control by biotechnological strategies.
650 $aCurculionidae
650 $aEnzymatic hydrolysis
650 $aGene expression
650 $aInsect pests
650 $aStarch
650 $aAmido
650 $aCurculionideo
650 $aHidrolise
650 $aPraga de planta
653 $aExpressão gênica
700 1 $aMACEDO, L. L. P.
700 1 $aAMORIM, T. M. L.
700 1 $aSANTOS, V. O.
700 1 $aFRAGOSO, R. da R.
700 1 $aLUCENA, W. A.
700 1 $aMENEGUIM, A. M.
700 1 $aVALENCIA-JIMENEZ, A.
700 1 $aENGLER, G.
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
700 1 $aFREIRE, E. V. S. A.
773 $tGene$gv. 553, n. 1, p. 7-16, Dec. 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Cerrados (CPAC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Amazônia Ocidental. |
|
Data corrente: |
01/06/2005 |
|
Data da última atualização: |
07/11/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Nacional - A |
|
Autoria: |
MOREIRA, A.; GARCIA, A. G. y; HEINRICHS, R.; MALAVOLTA, E. |
|
Afiliação: |
ADONIS MOREIRA, CPAA. |
|
Título: |
Influência do magnésio, boro e manganês na absorção de zinco por raízes destacadas de duas cultivares de arroz. |
|
Ano de publicação: |
2003 |
|
Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 24, n. 2, p. 213-218, jul./dez. 2003. |
|
Idioma: |
Português |
|
Conteúdo: |
Os efeitos do magnésio, boro e manganês na absorção de zinco foram estudados usando-se o método de cinética de absorção com Zn65 por raízes destacadas das cultivares de arroz IAC 165 e IAC 201. |
|
Palavras-Chave: |
Cinética de absorção; Oriza sativa. |
|
Thesagro: |
Arroz. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191787/1/arroz-1.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/125280/1/2177-7118-1-PB.pdf
|
|
Marc: |
LEADER 00803naa a2200193 a 4500
001 2105202
005 2019-11-07
008 2003 bl uuuu u00u1 u #d
100 1 $aMOREIRA, A.
245 $aInfluência do magnésio, boro e manganês na absorção de zinco por raízes destacadas de duas cultivares de arroz.
260 $c2003
520 $aOs efeitos do magnésio, boro e manganês na absorção de zinco foram estudados usando-se o método de cinética de absorção com Zn65 por raízes destacadas das cultivares de arroz IAC 165 e IAC 201.
650 $aArroz
653 $aCinética de absorção
653 $aOriza sativa
700 1 $aGARCIA, A. G. y
700 1 $aHEINRICHS, R.
700 1 $aMALAVOLTA, E.
773 $tSemina: Ciências Agrárias, Londrina$gv. 24, n. 2, p. 213-218, jul./dez. 2003.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
