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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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Data corrente: |
26/06/2017 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, L. F. da; GUERRA, C. C.; CZERMAINSKI, A. B. C.; FERRARI, L.; BERGOLD, A. M. |
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Afiliação: |
LETICIA FLORES DA SILVA, CNPUV; CELITO CRIVELLARO GUERRA, CNPUV; ANA BEATRIZ COSTA CZERMAINSKI, CNPUV; Leonardo Ferrari, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Campus Bento Gonçalves, Avenida Osvaldo Aranha, n o 540, CEP 95700-000 Bento Gonçalves, RS, Brazil. E-mail: leonardo.enologo@gmail.com; Ana Maria Bergold, Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Laboratório de Química Farmacêutica, Avenida Ipiranga, n o 2.752, CEP 90610-000 Porto Alegre, RS, Brazil. E-mail: ana.bergold@ufrgs.b. |
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Título: |
Validation of a chromatographic method to routine analysis of transresveratrol and quercetin in red wines. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, v. 51, n. 5, p. 335-343, maio 2017. |
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DOI: |
10.1590/S0100-204X2017000500007 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit: specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines. Index terms: Vitis vinifera , high-pressure liquid chromatography, polyphenol, quantification. Validação de método cromatográfico para análises de rotina de trans-resveratrol e quercetina em vinhos tintos O objetivo deste trabalho foi validar um método rápido, com baixo consumo de solventes, para a análise de trans-resveratrol e quercetina em vinhos tintos. Os vinhos foram elaborados por método clássico, a partir das cultivares de uva (Vitis vinifera) Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat e Tempranillo. As amostras foram filtradas e analisadas por cromatografia líquida de alta eficiência (CLAE) com detector de arranjo de diodos (DAD), em 306 e 371 nm, quanto ao trans-resveratrol e à quercetina, respectivamente. Utilizou-se uma coluna octilsilano, e a fase móvel foi composta por um gradiente de metanol, água e ácido fórmico. O método foi validado de acordo com as seguintes figuras de mérito: especificidade, linearidade, limite de detecção, limite de quantificação, precisão, exatidão e robustez. O método CLAE-DAD proposto pode ser implantado para análises de transresveratrol e quercetina em vinhos tintos. Termos para indexação: Vitis vinifera, cromatografia líquida de alta eficiência, polifenol, quantificação. MenosThe objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit: specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines. Index terms: Vitis vinifera , high-pressure liquid chromatography, polyphenol, quantification. Validação de método cromatográfico para análises de rotina de trans-resveratrol e quercetina em vinhos tintos O objetivo deste trabalho foi validar um método rápido, com baixo consumo de solventes, para a análise de trans-resveratrol e quercetina em vinhos tintos. Os vinhos foram elaborados por método clássico, a partir das cultivares de uva (Vitis vinifera) Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabe... Mostrar Tudo |
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Palavras-Chave: |
Cabernet Sauvignon; Cromatografia líquida de alta eficiência; Cultivars Cabernet Franc; High-pressure liquid chromatography; Malbec; Merlot; Petit Verdot; Pinot Noir; Polifenol oxidase; Quantificação; Quantification; Ruby Cabernet; Syrah; Tannat; Tempranillo. |
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Thesagro: |
Vitis Vinifera. |
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Thesaurus Nal: |
Polyphenols. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161153/1/Published-PAB-2017.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161076/1/Validation-of-a-chromatographic.pdf
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Marc: |
LEADER 03415naa a2200385 a 4500
001 2071659
005 2019-05-06
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2017000500007$2DOI
100 1 $aSILVA, L. F. da
245 $aValidation of a chromatographic method to routine analysis of transresveratrol and quercetin in red wines.
260 $c2017
520 $aThe objective of this work was to validate a fast method with low-solvent use, for the analysis of trans-resveratrol and quercetin in red wines. The wines were prepared, using a classical method, from the grape (Vitis vinifera) cultivars Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat, and Tempranillo. Samples were filtered and analyzed by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD), at 306 and 371 nm, for trans-resveratrol and quercetin, respectively. An octylsilane column was used, and the mobile phase was composed by a gradient of methanol, water, and formic acid. The method was validated according to the following figures of merit: specificity, linearity, limit of detection, limit of quantification, precision, accuracy, and robustness. The proposed HPLC-DAD method may be established for the analysis of trans-resveratrol and quercetin in red wines. Index terms: Vitis vinifera , high-pressure liquid chromatography, polyphenol, quantification. Validação de método cromatográfico para análises de rotina de trans-resveratrol e quercetina em vinhos tintos O objetivo deste trabalho foi validar um método rápido, com baixo consumo de solventes, para a análise de trans-resveratrol e quercetina em vinhos tintos. Os vinhos foram elaborados por método clássico, a partir das cultivares de uva (Vitis vinifera) Cabernet Franc, Cabernet Sauvignon, Malbec, Merlot, Petit Verdot, Pinot Noir, Ruby Cabernet, Syrah, Tannat e Tempranillo. As amostras foram filtradas e analisadas por cromatografia líquida de alta eficiência (CLAE) com detector de arranjo de diodos (DAD), em 306 e 371 nm, quanto ao trans-resveratrol e à quercetina, respectivamente. Utilizou-se uma coluna octilsilano, e a fase móvel foi composta por um gradiente de metanol, água e ácido fórmico. O método foi validado de acordo com as seguintes figuras de mérito: especificidade, linearidade, limite de detecção, limite de quantificação, precisão, exatidão e robustez. O método CLAE-DAD proposto pode ser implantado para análises de transresveratrol e quercetina em vinhos tintos. Termos para indexação: Vitis vinifera, cromatografia líquida de alta eficiência, polifenol, quantificação.
650 $aPolyphenols
650 $aVitis Vinifera
653 $aCabernet Sauvignon
653 $aCromatografia líquida de alta eficiência
653 $aCultivars Cabernet Franc
653 $aHigh-pressure liquid chromatography
653 $aMalbec
653 $aMerlot
653 $aPetit Verdot
653 $aPinot Noir
653 $aPolifenol oxidase
653 $aQuantificação
653 $aQuantification
653 $aRuby Cabernet
653 $aSyrah
653 $aTannat
653 $aTempranillo
700 1 $aGUERRA, C. C.
700 1 $aCZERMAINSKI, A. B. C.
700 1 $aFERRARI, L.
700 1 $aBERGOLD, A. M.
773 $tPesquisa Agropecuária Brasileira$gv. 51, n. 5, p. 335-343, maio 2017.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/10/2014 |
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Data da última atualização: |
27/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
HUFNAGEL, B.; SOUSA, S. M. de; ASSIS, L.; GUIMARAES, C. T.; LEISER, W.; AZEVEDO, G. C.; NEGRI, B.; LARSON, B. G.; SHAFF, J. E.; PASTINA, M. M.; BARROS, B. A.; WELTZIEN, E.; RATTUNDE, H. F. W.; VIANA, J. H.; CLARK, R. T.; FALCÃO, A.; GAZAFFI, R.; GARCIA, A. A. F.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. |
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Afiliação: |
SYLVIA MORAIS DE SOUSA TINOCO, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; MARIA MARTA PASTINA, CNPMS; BEATRIZ DE ALMEIDA BARROS, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
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Título: |
Duplicate and conquer: multiple homologs of PHOSPHORUS-STARVATION TOLERANCE1 enhance phosphorus acquisition and sorghum performance on low-phosphorus soils. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Physiology, Bethesda, v. 166, p. 659-677, Oct. 2014. |
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DOI: |
10.1104/pp.114.243949 |
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Idioma: |
Português |
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Conteúdo: |
Low soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice (Oryza sativa) proteinkinase,PHOSPHORUS-STARVATION TOLERANCE1 (OsPSTOL1), was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum (Sorghum bicolor) performance under low P. Association mapping was undertaken in two sorghum association panels phenotyped for P uptake, root system morphology and architecture in hydroponics and grain yield and biomass accumulation under low-P conditions, in Brazil and/or in Mali. Root length and root surface area were positively correlated with grain yield under low P in the soil, emphasizing the importance of P acquisition efficiency in sorghum adaptation to low-P availability. SbPSTOL1 alleles reducing root diameter were associated with enhanced P uptake under low P in hydroponics, whereas Sb03g006765 and Sb03g0031680 alleles increasing root surface area also increased grain yield in a low-P soil. SbPSTOL1 genes colocalized with quantitative trait loci for traits underlying root morphology and dry weight accumulation under low P via linkage mapping. Consistent allelic effects for enhanced sorghum performance under low P between association panels, including enhanced grain yield under low P in the soil in Brazil, point toward a relatively stable role for Sb03g006765 across genetic backgrounds and environmental conditions. This study indicates that multiple SbPSTOL1 genes have a more general role in the root system, not only enhancing root morphology traits but also changing root system architecture, which leads to grain yield gain under low-P availability in the soil. MenosLow soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice (Oryza sativa) proteinkinase,PHOSPHORUS-STARVATION TOLERANCE1 (OsPSTOL1), was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum (Sorghum bicolor) performance under low P. Association mapping was undertaken in two sorghum association panels phenotyped for P uptake, root system morphology and architecture in hydroponics and grain yield and biomass accumulation under low-P conditions, in Brazil and/or in Mali. Root length and root surface area were positively correlated with grain yield under low P in the soil, emphasizing the importance of P acquisition efficiency in sorghum adaptation to low-P availability. SbPSTOL1 alleles reducing root diameter were associated with enhanced P uptake under low P in hydroponics, whereas Sb03g006765 and Sb03g0031680 alleles increasing root surface area also increased grain yield in a low-P soil. SbPSTOL1 genes colocalized with quantitative trait loci for traits underlying root morphology and dry weight accumulation under low P via linkage mapping. Consistent allelic effects for enhanced sorghum performance under low P between association panels, including enhanced grain yield under low P in the soil in Brazil, point toward a relatively stable role for Sb03g006765 across genetic backgrounds and environmental conditions. This study indicates th... Mostrar Tudo |
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Thesagro: |
Fósforo; Solo; Sorgo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02900naa a2200409 a 4500
001 1997010
005 2017-09-27
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1104/pp.114.243949$2DOI
100 1 $aHUFNAGEL, B.
245 $aDuplicate and conquer$bmultiple homologs of PHOSPHORUS-STARVATION TOLERANCE1 enhance phosphorus acquisition and sorghum performance on low-phosphorus soils.$h[electronic resource]
260 $c2014
520 $aLow soil phosphorus (P) availability is a major constraint for crop production in tropical regions. The rice (Oryza sativa) proteinkinase,PHOSPHORUS-STARVATION TOLERANCE1 (OsPSTOL1), was previously shown to enhance P acquisition and grain yield in rice under P deficiency. We investigated the role of homologs of OsPSTOL1 in sorghum (Sorghum bicolor) performance under low P. Association mapping was undertaken in two sorghum association panels phenotyped for P uptake, root system morphology and architecture in hydroponics and grain yield and biomass accumulation under low-P conditions, in Brazil and/or in Mali. Root length and root surface area were positively correlated with grain yield under low P in the soil, emphasizing the importance of P acquisition efficiency in sorghum adaptation to low-P availability. SbPSTOL1 alleles reducing root diameter were associated with enhanced P uptake under low P in hydroponics, whereas Sb03g006765 and Sb03g0031680 alleles increasing root surface area also increased grain yield in a low-P soil. SbPSTOL1 genes colocalized with quantitative trait loci for traits underlying root morphology and dry weight accumulation under low P via linkage mapping. Consistent allelic effects for enhanced sorghum performance under low P between association panels, including enhanced grain yield under low P in the soil in Brazil, point toward a relatively stable role for Sb03g006765 across genetic backgrounds and environmental conditions. This study indicates that multiple SbPSTOL1 genes have a more general role in the root system, not only enhancing root morphology traits but also changing root system architecture, which leads to grain yield gain under low-P availability in the soil.
650 $aFósforo
650 $aSolo
650 $aSorgo
700 1 $aSOUSA, S. M. de
700 1 $aASSIS, L.
700 1 $aGUIMARAES, C. T.
700 1 $aLEISER, W.
700 1 $aAZEVEDO, G. C.
700 1 $aNEGRI, B.
700 1 $aLARSON, B. G.
700 1 $aSHAFF, J. E.
700 1 $aPASTINA, M. M.
700 1 $aBARROS, B. A.
700 1 $aWELTZIEN, E.
700 1 $aRATTUNDE, H. F. W.
700 1 $aVIANA, J. H.
700 1 $aCLARK, R. T.
700 1 $aFALCÃO, A.
700 1 $aGAZAFFI, R.
700 1 $aGARCIA, A. A. F.
700 1 $aSCHAFFERT, R. E.
700 1 $aKOCHIAN, L. V.
700 1 $aMAGALHAES, J. V.
773 $tPlant Physiology, Bethesda$gv. 166, p. 659-677, Oct. 2014.
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Embrapa Milho e Sorgo (CNPMS) |
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