Data/hora: 09/06/2024 21:31:28

Biblioteca(s): | Embrapa Pecuária Sudeste.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 4
Data corrente: | 28/08/2023
Data da última atualização: | 28/08/2023
Autoria: | PORTO, B. L. S.; FARIA, I. D. L.; SOUZA, J. C. Q. DE; MOREIRA, O. B. DE O.; GAMA, M. A. S. da; OLIVEIRA, M. A. L. DE.
Afiliação: | BRENDA LEE SIMAS PORTO, Universidade Federal de Juiz de Fora; ISAURA DANIELE LEITE FARIA, Universidade Federal de Juiz de Fora; JÉSSICA CORDEIRO QUEIROZ DE SOUZA, Universidade Federal de Juiz de Fora; OLÍVIA BRITO DE OLIVEIRA MOREIRA, Universidade Federal de Juiz de Fora; MARCO ANTONIO SUNDFELD DA GAMA, CPPSE; MARCONE AUGUSTO LEAL DE OLIVEIRA, Universidade Estadual de Campinas.
Título: | Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID.
Ano de publicação: | 2023
Fonte/Imprenta: | Química Nova, v. 7, p. 743-746, 2023.
DOI: | 10.21577/0100-4042.20230026
Idioma: | Inglês
Conteúdo: | Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency.
Palavras-Chave: | CLA; Fast GC; SLB IL111.
Thesaurus Nal: | Fatty acids; Milk.
Categoria do assunto: | W Química e Física
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156165/1/RapidMethod.pdf
Registro original: | Embrapa Pecuária Sudeste (CPPSE)
Exemplares: | CPPSE - (26173-1) - UPC - AP - DD - PROCI-2023.00054 - POR - 2023.00214 - DIS