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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
01/08/2016 |
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Data da última atualização: |
01/08/2016 |
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Autoria: |
FONSECA, E. T. da; GALINDO, L. T.; PORCIONATTO, M. A.; MIGLINO, M. A. |
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Afiliação: |
ERIKA TOLEDO DA FONSECA, UFTT/DC/FMVZ/USP; LAYLA TESTA GALINDO, UNIFESP; MARIMÉLIA A. PORCIONATTO, UNIFESP; MARIA ÁNGELICA MIGLINO, DC/FMVZ/USP. |
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Título: |
Culture, characterization and differentiation of neural precursors from the central nervous system of guinea pigs (Cavia porcellus Linnaeus, 1758). |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 36 (supl. 1), p. 69-78, jun. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Potentially neurogenic areas were initially identified by incorporation of bromodeoxyuridine (BrdU) in cells underlying the subventricular zone (SVZ) of the lateral ventricles wall, hippocampus and olfactory bulbs of newborn guinea pigs. Neural precursors from the SVZ were cultured in suspension, generating neurospheres (NSFs), which, upon dissociation were able to generate new NSFs. Upon culture in the absence of growth factors, cells dissociated from NSFs displayed evidence for neural differentiation, giving rise to cells from neural lineage. Flow cytometry analysis for of NSFs-derived cells after differentiation revealed approximately 13.3% nestin positive, 5.5% Beta-III-tubulin positive, 9% GFAP positive and 7.8% mGalC positive. Functional assays by measurement of calcium influx upon gamma butiric amino acid (GABA) and glutamate stimuli, revealed stimulation in differentiated cells, an indicator of neuronal differentiation. The ability of guinea pig SVZ cells to originate functional neurons in vitro is promising for research and towards a future use of neural stem cells in the therapy of neurological disorders. |
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Palavras-Chave: |
Caracterização in vitro; Celula-tronco neural; Cobaio; In vitro characterization; Neural stem cells; Subventricular zone; Zona subventricular. |
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Thesagro: |
Produção vegetal. |
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Thesaurus NAL: |
Guinea pigs; In vitro culture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145947/1/Culture-characterization.pdf
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Marc: |
LEADER 02036naa a2200277 a 4500
001 2049957
005 2016-08-01
008 2016 bl uuuu u00u1 u #d
100 1 $aFONSECA, E. T. da
245 $aCulture, characterization and differentiation of neural precursors from the central nervous system of guinea pigs (Cavia porcellus Linnaeus, 1758).
260 $c2016
520 $aPotentially neurogenic areas were initially identified by incorporation of bromodeoxyuridine (BrdU) in cells underlying the subventricular zone (SVZ) of the lateral ventricles wall, hippocampus and olfactory bulbs of newborn guinea pigs. Neural precursors from the SVZ were cultured in suspension, generating neurospheres (NSFs), which, upon dissociation were able to generate new NSFs. Upon culture in the absence of growth factors, cells dissociated from NSFs displayed evidence for neural differentiation, giving rise to cells from neural lineage. Flow cytometry analysis for of NSFs-derived cells after differentiation revealed approximately 13.3% nestin positive, 5.5% Beta-III-tubulin positive, 9% GFAP positive and 7.8% mGalC positive. Functional assays by measurement of calcium influx upon gamma butiric amino acid (GABA) and glutamate stimuli, revealed stimulation in differentiated cells, an indicator of neuronal differentiation. The ability of guinea pig SVZ cells to originate functional neurons in vitro is promising for research and towards a future use of neural stem cells in the therapy of neurological disorders.
650 $aGuinea pigs
650 $aIn vitro culture
650 $aProdução vegetal
653 $aCaracterização in vitro
653 $aCelula-tronco neural
653 $aCobaio
653 $aIn vitro characterization
653 $aNeural stem cells
653 $aSubventricular zone
653 $aZona subventricular
700 1 $aGALINDO, L. T.
700 1 $aPORCIONATTO, M. A.
700 1 $aMIGLINO, M. A.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 36 (supl. 1), p. 69-78, jun. 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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