Data/hora: 08/06/2024 07:45:37

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 01/08/2016
Data da última atualização: | 01/08/2016
Autoria: | FONSECA, E. T. da; GALINDO, L. T.; PORCIONATTO, M. A.; MIGLINO, M. A.
Afiliação: | ERIKA TOLEDO DA FONSECA, UFTT/DC/FMVZ/USP; LAYLA TESTA GALINDO, UNIFESP; MARIMÉLIA A. PORCIONATTO, UNIFESP; MARIA ÁNGELICA MIGLINO, DC/FMVZ/USP.
Título: | Culture, characterization and differentiation of neural precursors from the central nervous system of guinea pigs (Cavia porcellus Linnaeus, 1758).
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 36 (supl. 1), p. 69-78, jun. 2016.
Idioma: | Inglês
Conteúdo: | Potentially neurogenic areas were initially identified by incorporation of bromodeoxyuridine (BrdU) in cells underlying the subventricular zone (SVZ) of the lateral ventricles wall, hippocampus and olfactory bulbs of newborn guinea pigs. Neural precursors from the SVZ were cultured in suspension, generating neurospheres (NSFs), which, upon dissociation were able to generate new NSFs. Upon culture in the absence of growth factors, cells dissociated from NSFs displayed evidence for neural differentiation, giving rise to cells from neural lineage. Flow cytometry analysis for of NSFs-derived cells after differentiation revealed approximately 13.3% nestin positive, 5.5% Beta-III-tubulin positive, 9% GFAP positive and 7.8% mGalC positive. Functional assays by measurement of calcium influx upon gamma butiric amino acid (GABA) and glutamate stimuli, revealed stimulation in differentiated cells, an indicator of neuronal differentiation. The ability of guinea pig SVZ cells to originate functional neurons in vitro is promising for research and towards a future use of neural stem cells in the therapy of neurological disorders.
Palavras-Chave: | Caracterização in vitro; Celula-tronco neural; Cobaio; In vitro characterization; Neural stem cells; Subventricular zone; Zona subventricular.
Thesagro: | Produção vegetal.
Thesaurus Nal: | Guinea pigs; In vitro culture.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/145947/1/Culture-characterization.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59811-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS