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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
10/08/2021 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROMERO TISOT, C. M.; ZANELLA, R.; SOUZA, A. P.; LÖF, L. M.; SOARES, J. C. M.; MARQUES, M. G.; ZANELLA, E. L. |
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Afiliação: |
CÂNDIDA MALDANER ROMERO TISOT, UPF; RICARDO ZANELLA, UPF; UDESC/Lages; LUCAS MALLMANN LÖF, UPF; UFSM; MARIANA GROKE MARQUES, CNPSA; ERALDO LOURENSO ZANELLA, UPF. |
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Título: |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020. |
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DOI: |
10.23880/oajvsr-16000202 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation. MenosAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addi... Mostrar Tudo |
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Palavras-Chave: |
Melatonina. |
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Thesagro: |
Criopreservação; Espermatozóide; Garanhão; Reprodução Animal. |
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Thesaurus Nal: |
Animal reproduction; Cryopreservation; Melatonin; Reactive oxygen species; Spermatozoa; Stallions. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225036/1/final9560.pdf
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Marc: |
LEADER 02592naa a2200337 a 4500
001 2133434
005 2021-08-10
008 2020 bl uuuu u00u1 u #d
024 7 $a10.23880/oajvsr-16000202$2DOI
100 1 $aROMERO TISOT, C. M.
245 $aMelatonin effect on cryopreserved sperm cells of crioulo stallions.$h[electronic resource]
260 $c2020
520 $aAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation.
650 $aAnimal reproduction
650 $aCryopreservation
650 $aMelatonin
650 $aReactive oxygen species
650 $aSpermatozoa
650 $aStallions
650 $aCriopreservação
650 $aEspermatozóide
650 $aGaranhão
650 $aReprodução Animal
653 $aMelatonina
700 1 $aZANELLA, R.
700 1 $aSOUZA, A. P.
700 1 $aLÖF, L. M.
700 1 $aSOARES, J. C. M.
700 1 $aMARQUES, M. G.
700 1 $aZANELLA, E. L
773 $tOpen Access Journal of Veterinary Science & Research$gv. 5, n. 2, 2020.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/02/2003 |
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Data da última atualização: |
09/02/2007 |
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Autoria: |
OLIVEIRA, M. C. N. de; TORRES, E.; GAZZIERO, D. L. P. GALERANI, P. R.; VASCONCELLOS, M. E. da C. |
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Título: |
Análise de dados com medidas repetidas no tempo. Experimento 1. Avaliação de sistemas de preparo do solo e semeadura da soja. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja - 1990/91.Londrina: EMBRAPA-CNPSo, 1996. |
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Volume: |
v. 2. |
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Páginas: |
p. 623-628. |
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Série: |
(EMBRAPA-CNPSo. Documentos, 99). |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00689naa a2200169 a 4500
001 1463509
005 2007-02-09
008 1996 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, M. C. N. de
245 $aAnálise de dados com medidas repetidas no tempo. Experimento 1. Avaliação de sistemas de preparo do solo e semeadura da soja.
260 $c1996
300 $ap. 623-628. v. 2.
490 $a(EMBRAPA-CNPSo. Documentos, 99).$vv. 2.
700 1 $aTORRES, E.
700 1 $aGAZZIERO, D. L. P. GALERANI, P. R.
700 1 $aVASCONCELLOS, M. E. da C.
773 $tIn: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja - 1990/91.Londrina: EMBRAPA-CNPSo, 1996.
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