Data/hora: 08/06/2024 10:34:24

Biblioteca(s): | Embrapa Agroenergia.
Data corrente: | 15/03/2019
Data da última atualização: | 18/11/2019
Autoria: | MESQUITA, L. M. de S.; RODRIGUES, C. F. B.; ROCHA, C. Q. da; BIANCHIM, M. S.; RODRIGUES, C. M.; OLIVEIRA, V. de; GAETA, H. H.; BELCHOR, M. N.; TOYAMA. M. H.; VILEGAS, W.
Afiliação: | Leonardo Mendes de Souza Mesquita; Caroline Fabri Bittencourt Rodrigues; Claudia Quintino da Rocha; Mayara Silveira Bianchim; CLENILSON MARTINS RODRIGUES, CNPAE; Vanda Maria de Oliveira; Henrique Hessel Gaeta; Mariana Novo Belchor; Marcos Hikari Toyama; Wagner Vilegas.
Título: | LC-ESI-IT-MS/MS and MALDI-TOF approach: identification of natural polymers from rhizophora mangle barks and determination of their analgesic and anti-inflammatory properties.
Ano de publicação: | 2019
Fonte/Imprenta: | Natural Products and Bioprospecting, v. 9, p. 23-34, 2019.
Idioma: | Inglês
Conteúdo: | We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)?ESI?IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]?FIA?IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 ? 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins?m/z 873?3465 Da) and ? 16 Da (homologous series of gallocatechins?m/z 905?3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779?3371) and glucoafzelechins (m/z 747?3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 lg mL-1) have a morphine-like effect and intense anti-inflammatory activity.
Palavras-Chave: | Anti-inflammatory; Catechins; Mangroves; Morphine-like effect.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/194310/1/CLENILSON-041Natural-Products-and-Bioprospecting.pdf
Registro original: | Embrapa Agroenergia (CNPAE)
Exemplares: | CNPAE - (3444-1) - UPC - AP - DD -  -  -  - DIS