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| Registros recuperados : 134 | |
| 121. |  | PRESTES, A. A.; SILVEIRA, M. F.; CANELLA, M. H. M.; HELM, C. V.; ANDRADE, D. R. M.; FERREIRA, A. L. A.; AMBONI, R. D. de M. C.; FEDRIGO, I. M. T.; HERNÁNDEZ, E.; PRUDENCIO, E. S. Whey block freeze concentration aiming a functional fermented lactic beverage with the addition of probiotic and guabiroba pulp (Campomanesia xanthocarpa O. Berg), a native Brazilian fruit. Food Science and Technology, v. 43, e26923, p. 1-9, 2023.| Biblioteca(s): Embrapa Florestas. |
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| 122. | | LEÃO, J. M.; COELHO, S. G.; LAGE, C. F. de A.; AZEVEDO, R. A. de; LIMA, J. A. M.; CARNEIRO, J. C.; FERREIRA, A. L.; MACHADO, F. S.; PEREIRA, L. G. R.; TOMICH, T. R.; DINIZ NETO, H. do C.; CAMPOS, M. M. How divergence for feed efficiency traits affects body measurements and metabolites in blood and ruminal parameters on pre-weaning dairy heifers. Animals, v. 11, 3436, 2021.| Biblioteca(s): Embrapa Gado de Leite. |
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| 123. | | SILVA, B. A. de O.; FERREIRA, A. L.; ACUNHA, R. M. G.; ALMEIDA, R. G. dos S.; SANTOS, J. G. dos; FERNANDES, C. E.; CHAVES, F. C. M.; CHAGAS, E. C.; CARDOSO, C. A. L.; CAMPOS, C. M. de; COSTA, D. C. Anesthetic efficiency of essential oil of Zingiber officinale for Astyanax lacustris: induction time, recovery time, ventilatory frequency, and gill histopathology. Aquaculture International, 2023. Online first.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 124. | | LIMA, L. M. C. A. de; GOES, M. H. de B.; SILVA, J. X. da; RAMOS, V. A.; COSTA, J. de A.; CALHEIROS, S. Q. C.; FERREIRA, A. L.; SILVA, A. P. L. da; SILVA, J. P.; BARROS, M. de S.; CAVALCANTE, M. S. G. Aplicacao da carta geoprocessada de proximidade no planejamento ambiental de Maceio-AL. In: SEMANA ESTADUAL DE GEOPROCESSAMENTO, 1., 1996, Rio de Janeiro. Geoprocessamento: mitos e realidade. Rio de Janeiro: FGeo-RJ, 1996. p.141-146| Biblioteca(s): Embrapa Agricultura Digital. |
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| 125. | | LIMA, L. M. C. A.; SILVA, J. X. da; GOES, M. H. B.; CALHEIROS, S. Q.; COSTA, J. de A.; RAMOS, V. A.; GOES FILHO, J. P.; FERREIRA, A. L.; BERGAMO, R.; GUIMARAES JUNIOR, S. A.; SANTOS, L. R. dos; SANTOS, J. V. dos; LIMA, V. P. de. Maceio: potencial turistico com base na tecnologia de sistema geografico de informacao. In: SEMANA ESTADUAL DE GEOPROCESSAMENTO, 1., 1996, Rio de Janeiro. Geoprocessamento: mitos e realidade. Rio de Janeiro: FGeo-RJ, 1996. p.246-253| Biblioteca(s): Embrapa Agricultura Digital. |
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| 126. | | SACRAMENTO, J. P.; OLIVEIRA, A. S.; LEÃO, A. E.; FONSECA, A. P.; COELHO, S. G.; TOMICH, T. R.; MACHADO, F. S.; FERREIRA, A. L.; CAMPOS, M. M.; SILVI, R. R.; ROWNTREE, J. E.; RODRIGUES, J. R. P.; PEREIRA, L. G. R. Energy requirement for primiparous Holstein × Gyr crossbred dairy cows. Journal of Dairy Science, 2024.| Biblioteca(s): Embrapa Gado de Leite. |
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| 127. | | RESENDE, A. S. de; FERREIRA, A. L.; PEDREIRA, B. da C. C. G.; JANTALIA, C. P.; CAMPELLO, E. F. C.; FIDALGO, E. C. C.; BALIEIRO, F. de C.; DONAGEMMA, G. K.; CURCIO, G. R.; SOUSA, L. P. de; CORREIA, M. E. F.; PRADO, R. B.; DEDECEK, R. A.; CARVALHO JÚNIOR, W. de (coord.). Corredor ecológico do COMPERJ: um caminho verde para o COMPERJ. Rio de Janeiro: Embrapa Agrobiologia: Colombo: Embrapa Florestas; Rio de Janeiro: Embrapa Solos, 2007. 39 p.| Biblioteca(s): Embrapa Florestas. |
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| 128. | | RESENDE, A. S. de; FERREIRA, A. L.; PEDREIRA, B. da C. C. G.; JANTALIA, C. P.; CAMPELLO, E. F. C.; FIDALGO, E. C. C.; BALIEIRO, F. de C.; DONAGEMMA, G. K.; CURCIO, G. R.; SOUSA, L. P. de; CORREIA, M. E. F.; PRADO, R. B.; DEDECEK, R. A.; CARVALHO JÚNIOR, W. de (coord.). Corredor ecológico do COMPERJ: um caminho verde para o COMPERJ. Rio de Janeiro: Embrapa Agrobiologia: Colombo, PR: Embrapa Floresta; Rio de Janeiro: Embrapa Solos, 2007. 39 p.| Biblioteca(s): Embrapa Agrobiologia. |
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| 129. | | KOLLING, G. J.; STIVANIN, S. C. B.; GABBI, A. M.; MACHADO, F. S.; FERREIRA, A. L.; CAMPOS, M. M.; TOMICH, T. R.; CUNHA, C. S.; KLEIN, C. P.; AUGUST, P. M.; MATTÉ, C.; DILL, S. W.; PEREIRA, L. G. R.; FISCHER, V. Milk production and hematological and antioxidant profiles of dairy cows supplemented with oregano and green tea extracts as feed additives. Revista Brasileira de Zootecnia, v. 51, e20210150, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 130. | | RIBEIRO, R. S.; RODRIGUES, J. P. P.; MAURÍCIO, R. M.; BORGES, A. L. C. C.; REIS E SILVA, R.; BERCHIELLI, T. T.; VALADARES FILHO, S. C.; MACHADO, F. S.; CAMPOS, M. M.; FERREIRA, A. L.; GUIMARAES JUNIOR, R.; AZEVÊDO, J. A. G.; SANTOS, R. D. dos; TOMICH, T. R.; PEREIRA, L. G. R. Predicting enteric methane production from cattle in the tropics. Animal, 2020. 15 p.| Biblioteca(s): Embrapa Cerrados; Embrapa Gado de Leite; Embrapa Semiárido. |
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| 131. | | SOUSA, L. B.; PEREIRA, M. L. A.; SILVA, H. G. de O.; SOUSA, L. B.; SILVA, L. S. e; MACHADO, F. S.; TOMICH, T. R.; OSS, D. B.; FERREIRA, A. L.; CAMPOS, M. M.; COSTA, I. C.; PEREIRA, L. G. R. Prosopis juliflora piperidine alkaloid extract levels in diet for sheep change energy and nitrogen metabolism and affect enteric methane yield. Journal of the Science of Food and Agriculture, v. 102, p. 5132-5140, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 132. | | SILVEIRA, S. R.; TERRY, S. A.; BIFFIN, T. E.; MAURÍCIO, R. M.; PEREIRA, L. G. R.; FERREIRA, A. L.; RIBEIRO, R. S.; SACRAMENTO, J. P.; TOMICH, T. R.; MACHADO, F. S.; CAMPOS, M. M.; GAMA, M. A. S. da; CHAVES, A. V. Replacement of soybean meal with soybean cake reduces methane emissions in dairy cows and an assessment of a face-mask technique for methane measurement. Frontiers in Veterinary Science, v. 6, article 295, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 133. | | FERREIRA, A. L.; FARIA, J. C. de; MOURA, M. da C.; ZAIDEM, A. L. de M.; PIZETTA, C. S. R.; FREITAS, E. de O.; COELHO, G. R. C.; SILVA, J. F. A. e; BARRIGOSSI, J. A. F.; HOFFMANN, L. V.; SOUZA, T. L. P. O. de; ARAGÃO, F. J. L.; PINHEIRO, P. V. Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. Frontiers in Plant Science, v. 13, 984804, Aug. 2022.| Biblioteca(s): Embrapa Algodão; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 134. | | FONTANA, A.; PIRES, A. M. M.; VENTURIERI, A.; JESUS, A. H. de; PAULA, A. J. de; MEDEIROS, A. de S.; VARGAS, A. de G.; BERNDT, A.; FERREIRA, A. L.; LUIZ, A. J. B.; ALVES, A. E.; PEREIRA, A. C.; FREITAS, A. F. de; PACKER, A. P.; CAVALCANTE, A. P. C. M.; FRASCA, A. V. da L.; FERNANDES, A.; ZIMBRES, B.; RUDORFF, B. F. T.; DENARDI, B. M.; BORDRON, B.; ALVES, B. J. R.; KRAHEMBUHL, C. B. B. P.; ARAÚJO, C. E. R.; DUBEUX, C. B. S; MAEQUES, C. L.; SILVA, C. R.; BALDI, C.; JANTALIA, C. P.; GESTEIRA NETO, C. J. M.; BACK, C. D.; ANDRADE, C. A. de; AGUIAR, D. A. de; OBERLING, D. F.; OLIVEIRA, D. M. de; CALÇADO, D. M.; GONÇALVES, D. N. S.; MOLLETA, D. G. S.; GAROFALO, D. F. T.; FERREIRA, D.; GOULART, D.; OLIVEIRA, E. C. de; ROSA, E. R.; ALMEIDA, E. G. de; ALBUQUERQUE, E. R. G. M. de; LA ROVERE, E. L.; RIEGELHAUPT, E. M.; HENRIQUE, F. L.; FARIA, F. F. de; PATERNOST, F. F.; CESÁRIO, F. V.; PEREIRA, F. R. de S.; PAREYN, F. G. C.; RODRIGUES, G. A. H.; BERTANI, G.; LEMOS, G. da S.; RIBEIRO, G. H. P. de M.; CABRAL, G. H.; FROES, G. A. M.; GOES, G. V.; ANDRADE, G. S. de; CARVALHO, G. C. de; NASPOLINI, G. F.; CHRISTO, G. L. de; SANTOS, G. V. dos; BARROS, H. H. D. de; HASENACK, H.; TAVARES, H. C.; MARTINS, I. T.; FRANÇA, I. E. R.; ROITMAN, I.; SOARES, I. M. de M.; SIMIONATO, J.; FREITAS, J.; OMETTO, J. P. H. B.; LINARES, J. A. H.; RISSO, J.; CRUZ FILHO, J. L. V. da; QUINTÃO, J. M. B.; SILVA, J. S. O.; LANA, J. T. de O.; ZANATTA, J. A.; MARACAHIPES, L.; RIBEIRO, L. da S.; VICENTE, L. E.; SCHRAMM, L. F. P.; ROSSI, L. M. B.; SILVA, M. G. da; SARAIVA, M.; D'AGOSTO, M. de A.; VIRTUOSO, M. A.; LIGO, M. A. V.; RACHWAL, M. F. G.; ROSA, M.; PICHARILLO, M. E.; SILVA, M. G. L. da; MATSUURA, M. I. da S. F.; DALLA BETTA, M. M.; PEREIRA, M. J.; CARON, M. L.; ASSIS, M. L. R. de; SANTOS, M. M. de O.; ROCHA, M. B.; BUSTAMANTE, M. M. da C.; WALTER, M. C.; AQUINO, M. F. S. de; SALGADO, M. P. G.; SERRUYA, N. M.; MOREIRA, N. de P.; FERREIRA, N. C.; RAMOS, N. P.; CABRAL, O. M. R.; MOSER, P.; COLTURATO, P. D.; GRÜTZMACHER, P.; AUGUSTO, R. C.; GRISOLI, R. P. S.; SOLARI, R.; PAZIANOTTO, R. A. A.; DUPONT, R. A. B.; CANTINHO, R. Z.; PEIXOTO, R. de A.; PADILHA, R. A.; NICOLOSO, R. da S.; OLIVEIRA, R. R. S. de; HIGA, R. C. V.; GUIDOTTI, R. M. M.; NOGUEIRA, S. F.; MAIA, S. M. F.; ASSIS, T. F. de; IGAWA, T. K.; RODRIGUES, T. da S.; CARMO, T. R. L. do; MIRANDA, V. S.; KONDO, V. Y.; SCIVITTARO, W. B.; WILLS, W.; HOLLER, W. A. Inventário nacional de emissões e remoções antrópicas de gases de efeito estufa. In: BRASIL. Ministério da Ciência, Tecnologia e Inovações. Quarta Comunicação Nacional do Brasil à Convenção-Quadro das Nações Unidas sobre Mudança do Clima. Brasília, DF, 2021. cap. 2, p. 80-181.| Biblioteca(s): Embrapa Agrobiologia; Embrapa Amazônia Oriental; Embrapa Florestas; Embrapa Pecuária Sudeste; Embrapa Solos. |
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| Registros recuperados : 134 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/09/2022 |
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Data da última atualização: |
16/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, A. L.; FARIA, J. C. de; MOURA, M. da C.; ZAIDEM, A. L. de M.; PIZETTA, C. S. R.; FREITAS, E. de O.; COELHO, G. R. C.; SILVA, J. F. A. e; BARRIGOSSI, J. A. F.; HOFFMANN, L. V.; SOUZA, T. L. P. O. de; ARAGÃO, F. J. L.; PINHEIRO, P. V. |
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Afiliação: |
AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. |
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ISSN: |
1664-462X |
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DOI: |
https://doi.org/10.3389/fpls.2022.984804 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. MenosCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays we... Mostrar Tudo |
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Palavras-Chave: |
Dry bean; Insect pest management; VATPase. |
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Thesagro: |
Bemisia Tabaci; Feijão; Mosca Branca; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus NAL: |
Beans; RNA interference; Transgenic plants. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146488/1/fpls-984804.pdf
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Marc: |
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520 $aCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases.
650 $aBeans
650 $aRNA interference
650 $aTransgenic plants
650 $aBemisia Tabaci
650 $aFeijão
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773 $tFrontiers in Plant Science$gv. 13, 984804, Aug. 2022.
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Embrapa Arroz e Feijão (CNPAF) |
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