Data/hora: 08/06/2024 02:39:23

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	03/05/2010	
Data da última atualização: 	02/08/2023	
Autoria: 	OCHOA-CORONA, F. M.; LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J.	
Afiliação: 	Oklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	An RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	Phytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement	
Idioma: 	Inglês	
Notas: 	Abstracts of presentations, Annual Meeting of the APS 2009. p. S28.	
Conteúdo: 	Citrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine.	
Thesagro: 	Brevipalpus Phoenicis; Fruta Cítrica.	
Thesaurus Nal: 	Citrus.	
Categoria do assunto: 	G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748515/1/An-RT-PCR-procedure-for-detection-and-surveillance-of-Citrus-leprosis-virus-C-CiLV-C-in-post-entry-quarantine-stocks-of-citrus.pdf	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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