Data/hora: 08/06/2024 07:33:24

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 24/09/2003
Data da última atualização: | 30/03/2023
Autoria: | FREITAS-ASTUA, J.; ASTUA-MONGE, G.; POLSTON, J. E.; HIEBERT, E.
Afiliação: | Juliana Freitas-Astua, CNPMS; Gustavo Astua-Monge, Instituto Agronômico/Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira; Jane Elisabeth Polston, Universidade Fluminense - UF/Gulf Coast Research & Education Center; Ernest Hiebert, Universidade Fluminense - UF/Plant Pathology.
Título: | A simple and reliable method for the screening of transgenic tobacco plants.
Ano de publicação: | 2003
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 7, p. 893-896, jul. 2003
Idioma: | Inglês

Notas:

Notas Científicas.
Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas.

Conteúdo: | Even though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still time-consuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the polymerase chain reaction (PCR) amplification of the transgene using specific primers.
Palavras-Chave: | canamicina; organismo geneticamente modificado; teste.
Thesagro: | Planta Transgênica.
Thesaurus Nal: | genetically modified organisms; kanamycin; testing; transgenic plants.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/24881/1/v38n7a15.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (24881-1) - UPE - AP - DD - 630.72081 - P474 -  - DIS