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| Registros recuperados : 114 | |
| 41. |  | COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C. Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62. Abstract 109| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 42. | | PEREIRA, J. A.; ANTONIOLO-LUIZON, R.; LOCALI-FABRIS, E. C.; KUBO, K. S.; MACHADO, M. A.; FREITAS-ÁSTUA, J. Estudo da variabilidade genética da possível capa protéica do Citrus leprosis virus virus C (CiLV-C) em espécies de citros de 14 estados brasileiros. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 928.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 43. | | CUNHA, B. A.; NUNES, M. A.; FREITAS-ASTUA, J. de; BERGAMINI, M. P.; BASTIANEL, M.; NOVELLI, V. M. Different symptomatic tissues as sources of inoculum to citrus leprosis virus c (CILV-C). In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 128-PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 44. | | GUIDETTI-GONZALEZ, S.; FREITAS-ASTÚA, J.; AMARAL. A. M. do; MARTINS, N. F.; MEHTA, A.; SILVA, M. S.; CARRER, H. Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST). Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 943-956, 2007.| Biblioteca(s): Embrapa Cerrados. |
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| 48. | | NOVELLI, V. M.; FREITAS-ASTÚA, J.; SEGATTI, N.; HILF, M. E.; GOTTWALD, T. R.; MACHADO, M. A. Aquisição e inoculação do vírus da leprose dos citros (CiLV-C) por machos e fêmeas de Brevipalpus phoenicis. Fitopatologia Brasileira, Brasília, DF, v. 32, p. 293, ago. 2007. Suplemento. Edição dos Resumos do XL Congresso Brasiliero de Fitopatologia, Maringá, ago. 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 49. | | FREITAS-ÁSTUA, J.; FADEL, A. L.; BASTIANEL, M.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; MACHADO, M. A. Resposta diferencial de espécies e de híbridos de citros à leprose. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 809-814, jul. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 50. | | BASTIANEL, M.; FREITAS-ÁSTUA, J.; NICOLINI, F.; SEGATTI, N.; NOVELLI, V. M.; RODRIGUES, V.; MEDINA, C. L.; MACHADO, M. A. Response of mandarin cultivars and hybrids to citrus leprosis virus. Journal of Plant Pathology, Netherlands, v. 90, n. 2, p. 305-310, 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 51. | | KUBO, K. S.; STUART, R. M.; BASTIANEL, M.; COSTA, F. N.; FREITAS-ASTÚA, J.; MACHADO, M. A. Response of Murcott tangor and Pera sweet orange to Citrus leprosis virus C (CiLV-C) and Brevipalpus phoenicis mites analyzed by 2DE. In:JOINT ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS AND THE CANADIAN SOCIETY OF PLANT PHYSIOLOGISTS, 2009, Honolulu, Hawaii. Plant Biology Meeting: final program. [S.n.], 2009. P48071| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 52. | | LUCHETA, A. R.; SILVA-PINHATI, A. C. O.; BASÍLIO-PALMIERI, A. C.; BERGER, I. J.; FREITAS-ÁSTUA, J.; CRISTOFANI, M. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 819-831, 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 53. | | BERGER, I. J.; FREITAS-ÁSTUA, J.; REIS, M. S.; TARGON, M. L. P. N.; MACHADO, M. A. In silico prediction of gene expression patterns in Citrus flavedo. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 752-760, 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 54. | | KITAJIMA, E. W.; CALEGARIO, R. F.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; BASTIANEL, M.; FREITAS-ASTÚA, J.; FRANCISCHINI, F. In situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 55. | | KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD ROM| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 57. | | BASTIANEL, M.; PEREIRA, J. A.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; AZEVEDO, F. A.; FREITAS-ASTUA, J. Resposta de tangerinas e híbridos ao virus da leprose dos citros. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1458| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 58. | | NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 59. | | BERGER, I. J.; FREITAS-ÁSTUA, J.; SILVA-PINHATI, A. C. O.; BASILIO-PALMIERI, A. C.; LUCHETA, A. R.; NOVELLI, V. M.; MACHADO, M. A. Chalcone isomerase is likely encoded by a multigene family in citrus sinensis. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina, 2008. P21| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 60. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENÂNCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTÚA, J. Fluorescence images combined to chemometrics for the investigation of plants inoculated with candidatus liberibacter asiaticus In: CHEMOMETRICS IN ANALYTICAL CHEMISTRY - CAC, 12., 2010, Antwerp, Belgium. Book of abstracts... Antwerp: Universiteit Antwerpen, 2010.| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 114 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. |
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Afiliação: |
Marina França e Silva, CNPDIA; Débora Marcondes Bastos Pereira Miroli, CNPDIA; Marcelo C. B. Cardinali, CNPDIA; Yvonne P. Mascarenhas, USP; Polyanna kelly Martins, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Marcos Antonio Machado, APTA. |
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Título: |
Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Several diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection. MenosSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early ci... Mostrar Tudo |
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Palavras-Chave: |
Candidatus Liberibater asiaticus; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02492nam a2200265 a 4500
001 1872725
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aSILVA, M. F. e
245 $aApplication of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a120_PS2 Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aSeveral diseases have caused great damage to citrus production worldwide. Among them, Greening is the most feared for its aggressiveness, speed of spread, and a lack of resistant variety. Infected plants goes through a long asymptomatic period (around 6 to 36 months), allowing the permanence of inocullum in the field. This study purpose to evaluate the capability of early diagnosis of spectroscopy at infrared with transformed Fourier (FTIR) for the Greening disease. For both, 60 citrus seedlings were inoculated with Candidatus Liberibater asiaticus (CLas) and 60 healthy seedlings (control) were kept in a greenhouse, which grown under controlled conditions of soil, irrigation and nutrients. Their leaves were measured monthly using FTIR spectroscopy during eight months. The evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. The spectra were analyzed using Principal Component Analysis (PCA). For all the months, it was shown separation between the asymptomatic and healthy leaves, emphasizing that significant chemical changes are occurring before symptomatic phase. Preliminary analyses showed that infected plants suffered a decreasing in concentrations of carbohydrate and chlorophyll. The results also showed that is possible to recognize a typical signature of sick leaf using FTIR spectroscopy, even in symptomatic phase, which must be studied as a feasible tool to develop a methodology for early citrus greening detection.
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aCandidatus Liberibater asiaticus
653 $aPlant disease
700 1 $aMIROLI, D. M. B. P.
700 1 $aCARDINALI, M. C. B.
700 1 $aMASCARENHAS, Y. P.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS ASTUA, J. de
700 1 $aMACHADO, M. A.
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