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41. | | COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C. Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62. Abstract 109 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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42. | | PEREIRA, J. A.; ANTONIOLO-LUIZON, R.; LOCALI-FABRIS, E. C.; KUBO, K. S.; MACHADO, M. A.; FREITAS-ÁSTUA, J. Estudo da variabilidade genética da possível capa protéica do Citrus leprosis virus virus C (CiLV-C) em espécies de citros de 14 estados brasileiros. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 928. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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43. | | CUNHA, B. A.; NUNES, M. A.; FREITAS-ASTUA, J. de; BERGAMINI, M. P.; BASTIANEL, M.; NOVELLI, V. M. Different symptomatic tissues as sources of inoculum to citrus leprosis virus c (CILV-C). In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 128-PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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44. | | GUIDETTI-GONZALEZ, S.; FREITAS-ASTÚA, J.; AMARAL. A. M. do; MARTINS, N. F.; MEHTA, A.; SILVA, M. S.; CARRER, H. Genes associated with hypersensitive response (HR) in the citrus EST database (CitEST). Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 943-956, 2007. Biblioteca(s): Embrapa Cerrados. |
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48. | | NOVELLI, V. M.; FREITAS-ASTÚA, J.; SEGATTI, N.; HILF, M. E.; GOTTWALD, T. R.; MACHADO, M. A. Aquisição e inoculação do vírus da leprose dos citros (CiLV-C) por machos e fêmeas de Brevipalpus phoenicis. Fitopatologia Brasileira, Brasília, DF, v. 32, p. 293, ago. 2007. Suplemento. Edição dos Resumos do XL Congresso Brasiliero de Fitopatologia, Maringá, ago. 2007. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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49. | | FREITAS-ÁSTUA, J.; FADEL, A. L.; BASTIANEL, M.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; MACHADO, M. A. Resposta diferencial de espécies e de híbridos de citros à leprose. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 7, p. 809-814, jul. 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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50. | | BASTIANEL, M.; FREITAS-ÁSTUA, J.; NICOLINI, F.; SEGATTI, N.; NOVELLI, V. M.; RODRIGUES, V.; MEDINA, C. L.; MACHADO, M. A. Response of mandarin cultivars and hybrids to citrus leprosis virus. Journal of Plant Pathology, Netherlands, v. 90, n. 2, p. 305-310, 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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51. | | KUBO, K. S.; STUART, R. M.; BASTIANEL, M.; COSTA, F. N.; FREITAS-ASTÚA, J.; MACHADO, M. A. Response of Murcott tangor and Pera sweet orange to Citrus leprosis virus C (CiLV-C) and Brevipalpus phoenicis mites analyzed by 2DE. In:JOINT ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS AND THE CANADIAN SOCIETY OF PLANT PHYSIOLOGISTS, 2009, Honolulu, Hawaii. Plant Biology Meeting: final program. [S.n.], 2009. P48071 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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52. | | LUCHETA, A. R.; SILVA-PINHATI, A. C. O.; BASÍLIO-PALMIERI, A. C.; BERGER, I. J.; FREITAS-ÁSTUA, J.; CRISTOFANI, M. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 819-831, 2007. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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53. | | BERGER, I. J.; FREITAS-ÁSTUA, J.; REIS, M. S.; TARGON, M. L. P. N.; MACHADO, M. A. In silico prediction of gene expression patterns in Citrus flavedo. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 752-760, 2007. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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54. | | KITAJIMA, E. W.; CALEGARIO, R. F.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; BASTIANEL, M.; FREITAS-ASTÚA, J.; FRANCISCHINI, F. In situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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55. | | KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD ROM Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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57. | | BASTIANEL, M.; PEREIRA, J. A.; NOVELLI, V. M.; ANTONIOLI-LUIZON, R.; AZEVEDO, F. A.; FREITAS-ASTUA, J. Resposta de tangerinas e híbridos ao virus da leprose dos citros. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1458 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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58. | | NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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59. | | BERGER, I. J.; FREITAS-ÁSTUA, J.; SILVA-PINHATI, A. C. O.; BASILIO-PALMIERI, A. C.; LUCHETA, A. R.; NOVELLI, V. M.; MACHADO, M. A. Chalcone isomerase is likely encoded by a multigene family in citrus sinensis. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina, 2008. P21 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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60. | | PEREIRA, F. M. V.; MILORI, D. M. B. P.; PEREIRA-FILHO, E. R.; VENÂNCIO, A. L.; RUSSO, M. de S. T.; MARTINS, P. K.; FREITAS-ASTÚA, J. Fluorescence images combined to chemometrics for the investigation of plants inoculated with candidatus liberibacter asiaticus In: CHEMOMETRICS IN ANALYTICAL CHEMISTRY - CAC, 12., 2010, Antwerp, Belgium. Book of abstracts... Antwerp: Universiteit Antwerpen, 2010. Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 114 | |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
11/02/2008 |
Data da última atualização: |
12/02/2008 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - A |
Autoria: |
KITAJIMA, E. W.; GROOT, T. V. M.; NOVELLI, V. M.; FREITAS-ÁSTUA, J.; ALBERTI, G.; MORAES, G. J. de. |
Afiliação: |
Elliot W. Kitajima, ESALQ; Thomas V. M. Groot, UvA; Valdenice M. Novelli, APTA; Juliana Freitas-Astúa, CNPMF; Gerd Alberti; Gilberto J. de Moraes, ESALQ. |
Título: |
In situ observation of the Cardinium symbionts of Brevipalpus (Acari: Tenuipalpidae) by electron microscopy. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Experimental & Applied Acarology, v. 42, p. 263-271, 2007. |
Idioma: |
Português |
Conteúdo: |
Brevipalpus (Acari: Tenuipalpidae) mites are important pests on a variety of host plant species. The mites damage their hosts directly by feeding and some species also serve as vectors of plant viruses. Among more than 200 described Brevipalpus species, three are recognized as vectors of plant viruses: B. phoenicis, B. californicus and B. obovatus. These species occur worldwide in subtropical and tropical regions. Brevipalpus mites reproduce mostly by thelytokous parthenogenesis and this condition was attributed to a bacterial endosymbiont, recently characterized as a member of the genus Cardinium. The same symbiont infects many other arthropods and is capable of manipulating their host reproduction in various ways. Generally the presence of Cardinium is determined by molecular, PCR based, techniques. In the current work we present visual evidence for the presence of these bacteria by transmission electron microscopy as a complement of previous detection by PCR. Cardinium is easily identified by the presence of a unique array of microtubule-like structures (ML) in the cell. Symbionts have been observed in several organs and eggs from different populations of all three Brevipalpus species known as vector of plant viruses. Cardinium cells were always immersed directly within the cytoplasm of infected cells. Bacteria were observed in all females of all instars, but were absent from all males examined. Females from some Brevipalpus populations were observed to be uninfected by Cardinium. This observation confirmed previous PCR-based results that these populations were aposymbiotic. The observed distribution of the bacteria suggests that these bacteria could have other functions in the mite biology beside feminization. MenosBrevipalpus (Acari: Tenuipalpidae) mites are important pests on a variety of host plant species. The mites damage their hosts directly by feeding and some species also serve as vectors of plant viruses. Among more than 200 described Brevipalpus species, three are recognized as vectors of plant viruses: B. phoenicis, B. californicus and B. obovatus. These species occur worldwide in subtropical and tropical regions. Brevipalpus mites reproduce mostly by thelytokous parthenogenesis and this condition was attributed to a bacterial endosymbiont, recently characterized as a member of the genus Cardinium. The same symbiont infects many other arthropods and is capable of manipulating their host reproduction in various ways. Generally the presence of Cardinium is determined by molecular, PCR based, techniques. In the current work we present visual evidence for the presence of these bacteria by transmission electron microscopy as a complement of previous detection by PCR. Cardinium is easily identified by the presence of a unique array of microtubule-like structures (ML) in the cell. Symbionts have been observed in several organs and eggs from different populations of all three Brevipalpus species known as vector of plant viruses. Cardinium cells were always immersed directly within the cytoplasm of infected cells. Bacteria were observed in all females of all instars, but were absent from all males examined. Females from some Brevipalpus populations were observed to be uninfected by C... Mostrar Tudo |
Palavras-Chave: |
Aposymbiont; B californicus; B obovatus; B phoenicis; californicus; False spider mites; obovatus; phoenicis. |
Categoria do assunto: |
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Marc: |
LEADER 02523naa a2200277 a 4500 001 1654618 005 2008-02-12 008 2007 bl --- 0-- u #d 100 1 $aKITAJIMA, E. W. 245 $aIn situ observation of the Cardinium symbionts of Brevipalpus (Acari$bTenuipalpidae) by electron microscopy. 260 $c2007 520 $aBrevipalpus (Acari: Tenuipalpidae) mites are important pests on a variety of host plant species. The mites damage their hosts directly by feeding and some species also serve as vectors of plant viruses. Among more than 200 described Brevipalpus species, three are recognized as vectors of plant viruses: B. phoenicis, B. californicus and B. obovatus. These species occur worldwide in subtropical and tropical regions. Brevipalpus mites reproduce mostly by thelytokous parthenogenesis and this condition was attributed to a bacterial endosymbiont, recently characterized as a member of the genus Cardinium. The same symbiont infects many other arthropods and is capable of manipulating their host reproduction in various ways. Generally the presence of Cardinium is determined by molecular, PCR based, techniques. In the current work we present visual evidence for the presence of these bacteria by transmission electron microscopy as a complement of previous detection by PCR. Cardinium is easily identified by the presence of a unique array of microtubule-like structures (ML) in the cell. Symbionts have been observed in several organs and eggs from different populations of all three Brevipalpus species known as vector of plant viruses. Cardinium cells were always immersed directly within the cytoplasm of infected cells. Bacteria were observed in all females of all instars, but were absent from all males examined. Females from some Brevipalpus populations were observed to be uninfected by Cardinium. This observation confirmed previous PCR-based results that these populations were aposymbiotic. The observed distribution of the bacteria suggests that these bacteria could have other functions in the mite biology beside feminization. 653 $aAposymbiont 653 $aB californicus 653 $aB obovatus 653 $aB phoenicis 653 $acalifornicus 653 $aFalse spider mites 653 $aobovatus 653 $aphoenicis 700 1 $aGROOT, T. V. M. 700 1 $aNOVELLI, V. M. 700 1 $aFREITAS-ÁSTUA, J. 700 1 $aALBERTI, G. 700 1 $aMORAES, G. J. de 773 $tExperimental & Applied Acarology$gv. 42, p. 263-271, 2007.
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