Data/hora: 08/06/2024 04:08:55

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	09/07/2009	
Data da última atualização: 	14/06/2023	
Autoria: 	KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W.	
Afiliação: 	Karen Sumire Kubo, APTA; R. M. Stuart, APTA; Juliana Freitas-Ástua, CNPMF; Renata Antonioli-Luizon, APTA; Eliane Cristina Locali-Fabris, APTA; Helvecio Della Coletta-Filho, APTA; Marcos Antonio Machado, APTA; Elliot Watanabe Kitajima, ESALQ.	
Título: 	Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009.	
ISSN: 	0304-8608	
DOI: 	DOI 10.1007/s00705-009-0395-8	
Idioma: 	Inglês	
Conteúdo: 	The variability of a fragment of the nucleocapsid gene of orchid fleck virus (OFV) was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of 18 genera of orchids were collected from Brazil, Costa Rica and Australia. The SSCP analysis yielded six different band patterns, and phylogenetic analysis based on the nucleotide fragment sequence obtained in this work and six available in GenBank showed two different groups, one with isolates 023Germany and So-Japan, and other with the rest of the isolates. None of the analyses showed geographic correlation among the Brazilian strains. The data obtained in this study showed a low genetic variation in this region of the genome; the dN/dS ratio of 0.251-0.405 demonstrated a negative selective pressure that maintains the stability of the analyzed fragments.	
Palavras-Chave: 	Nuclear type.	
Thesaurus Nal: 	Orchid fleck virus.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (25700-1) - UPC - AP - DD - Publicação digital -  -  - DIS